Semi-Automated Hydrophobic Interaction Chromatography Column Scouting Used in the Two-Step Purification of Recombinant Green Fluorescent Protein

BACKGROUND: Hydrophobic interaction chromatography (HIC) most commonly requires experimental determination (i.e., scouting) in order to select an optimal chromatographic medium for purifying a given target protein. Neither a two-step purification of untagged green fluorescent protein (GFP) from crud...

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Detalles Bibliográficos
Autores principales: Stone, Orrin J., Biette, Kelly M., Murphy, Patrick J. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4177899/
https://www.ncbi.nlm.nih.gov/pubmed/25254496
http://dx.doi.org/10.1371/journal.pone.0108611

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