Cargando…
Flavonoid Fraction of Bergamot Juice Reduces LPS-Induced Inflammatory Response through SIRT1-Mediated NF-κB Inhibition in THP-1 Monocytes
Plant polyphenols exert anti-inflammatory activity through both anti-oxidant effects and modulation of pivotal pro-inflammatory genes. Recently, Citrus bergamia has been studied as a natural source of bioactive molecules with antioxidant activity, but few studies have focused on molecular mechanisms...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178028/ https://www.ncbi.nlm.nih.gov/pubmed/25260046 http://dx.doi.org/10.1371/journal.pone.0107431 |
_version_ | 1782336881538629632 |
---|---|
author | Risitano, Roberto Currò, Monica Cirmi, Santa Ferlazzo, Nadia Campiglia, Pietro Caccamo, Daniela Ientile, Riccardo Navarra, Michele |
author_facet | Risitano, Roberto Currò, Monica Cirmi, Santa Ferlazzo, Nadia Campiglia, Pietro Caccamo, Daniela Ientile, Riccardo Navarra, Michele |
author_sort | Risitano, Roberto |
collection | PubMed |
description | Plant polyphenols exert anti-inflammatory activity through both anti-oxidant effects and modulation of pivotal pro-inflammatory genes. Recently, Citrus bergamia has been studied as a natural source of bioactive molecules with antioxidant activity, but few studies have focused on molecular mechanisms underlying their potential beneficial effects. Several findings have suggested that polyphenols could influence cellular function by acting as activators of SIRT1, a nuclear histone deacetylase, involved in the inhibition of NF-κB signaling. On the basis of these observations we studied the anti-inflammatory effects produced by the flavonoid fraction of the bergamot juice (BJe) in a model of LPS-stimulated THP-1 cell line, focusing on SIRT1-mediated NF-κB inhibition. We demonstrated that BJe inhibited both gene expression and secretion of LPS-induced pro-inflammatory cytokines (IL-6, IL-1β, TNF-α) by a mechanism involving the inhibition of NF-κB activation. In addition, we showed that BJe treatment reversed the LPS-enhanced acetylation of p65 in THP-1 cells. Interestingly, increasing concentrations of Sirtinol were able to suppress the inhibitory effect of BJe via p65 acetylation, underscoring that NF-κB–mediated inflammatory cytokine production may be directly linked to SIRT1 activity. These results suggest that BJe may be useful for the development of alternative pharmacological strategies aimed at reducing the inflammatory process. |
format | Online Article Text |
id | pubmed-4178028 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41780282014-10-02 Flavonoid Fraction of Bergamot Juice Reduces LPS-Induced Inflammatory Response through SIRT1-Mediated NF-κB Inhibition in THP-1 Monocytes Risitano, Roberto Currò, Monica Cirmi, Santa Ferlazzo, Nadia Campiglia, Pietro Caccamo, Daniela Ientile, Riccardo Navarra, Michele PLoS One Research Article Plant polyphenols exert anti-inflammatory activity through both anti-oxidant effects and modulation of pivotal pro-inflammatory genes. Recently, Citrus bergamia has been studied as a natural source of bioactive molecules with antioxidant activity, but few studies have focused on molecular mechanisms underlying their potential beneficial effects. Several findings have suggested that polyphenols could influence cellular function by acting as activators of SIRT1, a nuclear histone deacetylase, involved in the inhibition of NF-κB signaling. On the basis of these observations we studied the anti-inflammatory effects produced by the flavonoid fraction of the bergamot juice (BJe) in a model of LPS-stimulated THP-1 cell line, focusing on SIRT1-mediated NF-κB inhibition. We demonstrated that BJe inhibited both gene expression and secretion of LPS-induced pro-inflammatory cytokines (IL-6, IL-1β, TNF-α) by a mechanism involving the inhibition of NF-κB activation. In addition, we showed that BJe treatment reversed the LPS-enhanced acetylation of p65 in THP-1 cells. Interestingly, increasing concentrations of Sirtinol were able to suppress the inhibitory effect of BJe via p65 acetylation, underscoring that NF-κB–mediated inflammatory cytokine production may be directly linked to SIRT1 activity. These results suggest that BJe may be useful for the development of alternative pharmacological strategies aimed at reducing the inflammatory process. Public Library of Science 2014-09-26 /pmc/articles/PMC4178028/ /pubmed/25260046 http://dx.doi.org/10.1371/journal.pone.0107431 Text en © 2014 Risitano et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Risitano, Roberto Currò, Monica Cirmi, Santa Ferlazzo, Nadia Campiglia, Pietro Caccamo, Daniela Ientile, Riccardo Navarra, Michele Flavonoid Fraction of Bergamot Juice Reduces LPS-Induced Inflammatory Response through SIRT1-Mediated NF-κB Inhibition in THP-1 Monocytes |
title | Flavonoid Fraction of Bergamot Juice Reduces LPS-Induced Inflammatory Response through SIRT1-Mediated NF-κB Inhibition in THP-1 Monocytes |
title_full | Flavonoid Fraction of Bergamot Juice Reduces LPS-Induced Inflammatory Response through SIRT1-Mediated NF-κB Inhibition in THP-1 Monocytes |
title_fullStr | Flavonoid Fraction of Bergamot Juice Reduces LPS-Induced Inflammatory Response through SIRT1-Mediated NF-κB Inhibition in THP-1 Monocytes |
title_full_unstemmed | Flavonoid Fraction of Bergamot Juice Reduces LPS-Induced Inflammatory Response through SIRT1-Mediated NF-κB Inhibition in THP-1 Monocytes |
title_short | Flavonoid Fraction of Bergamot Juice Reduces LPS-Induced Inflammatory Response through SIRT1-Mediated NF-κB Inhibition in THP-1 Monocytes |
title_sort | flavonoid fraction of bergamot juice reduces lps-induced inflammatory response through sirt1-mediated nf-κb inhibition in thp-1 monocytes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178028/ https://www.ncbi.nlm.nih.gov/pubmed/25260046 http://dx.doi.org/10.1371/journal.pone.0107431 |
work_keys_str_mv | AT risitanoroberto flavonoidfractionofbergamotjuicereduceslpsinducedinflammatoryresponsethroughsirt1mediatednfkbinhibitioninthp1monocytes AT curromonica flavonoidfractionofbergamotjuicereduceslpsinducedinflammatoryresponsethroughsirt1mediatednfkbinhibitioninthp1monocytes AT cirmisanta flavonoidfractionofbergamotjuicereduceslpsinducedinflammatoryresponsethroughsirt1mediatednfkbinhibitioninthp1monocytes AT ferlazzonadia flavonoidfractionofbergamotjuicereduceslpsinducedinflammatoryresponsethroughsirt1mediatednfkbinhibitioninthp1monocytes AT campigliapietro flavonoidfractionofbergamotjuicereduceslpsinducedinflammatoryresponsethroughsirt1mediatednfkbinhibitioninthp1monocytes AT caccamodaniela flavonoidfractionofbergamotjuicereduceslpsinducedinflammatoryresponsethroughsirt1mediatednfkbinhibitioninthp1monocytes AT ientilericcardo flavonoidfractionofbergamotjuicereduceslpsinducedinflammatoryresponsethroughsirt1mediatednfkbinhibitioninthp1monocytes AT navarramichele flavonoidfractionofbergamotjuicereduceslpsinducedinflammatoryresponsethroughsirt1mediatednfkbinhibitioninthp1monocytes |