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Identification of Rabbit Annulus Fibrosus-Derived Stem Cells
Annulus fibrosus (AF) injuries can lead to substantial deterioration of intervertebral disc (IVD) which characterizes degenerative disc disease (DDD). However, treatments for AF repair/regeneration remain challenging due to the intrinsic heterogeneity of AF tissue at cellular, biochemical, and biome...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178129/ https://www.ncbi.nlm.nih.gov/pubmed/25259600 http://dx.doi.org/10.1371/journal.pone.0108239 |
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author | Liu, Chen Guo, Qianping Li, Jun Wang, Shenghao Wang, Yibin Li, Bin Yang, Huilin |
author_facet | Liu, Chen Guo, Qianping Li, Jun Wang, Shenghao Wang, Yibin Li, Bin Yang, Huilin |
author_sort | Liu, Chen |
collection | PubMed |
description | Annulus fibrosus (AF) injuries can lead to substantial deterioration of intervertebral disc (IVD) which characterizes degenerative disc disease (DDD). However, treatments for AF repair/regeneration remain challenging due to the intrinsic heterogeneity of AF tissue at cellular, biochemical, and biomechanical levels. In this study, we isolated and characterized a sub-population of cells from rabbit AF tissue which formed colonies in vitro and could self-renew. These cells showed gene expression of typical surface antigen molecules characterizing mesenchymal stem cells (MSCs), including CD29, CD44, and CD166. Meanwhile, they did not express negative markers of MSCs such as CD4, CD8, and CD14. They also expressed Oct-4, nucleostemin, and SSEA-4 proteins. Upon induced differentiation they showed typical osteogenesis, chondrogenesis, and adipogenesis potential. Together, these AF-derived colony-forming cells possessed clonogenicity, self-renewal, and multi-potential differentiation capability, the three criteria characterizing MSCs. Such AF-derived stem cells may potentially be an ideal candidate for DDD treatments using cell therapies or tissue engineering approaches. |
format | Online Article Text |
id | pubmed-4178129 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41781292014-10-02 Identification of Rabbit Annulus Fibrosus-Derived Stem Cells Liu, Chen Guo, Qianping Li, Jun Wang, Shenghao Wang, Yibin Li, Bin Yang, Huilin PLoS One Research Article Annulus fibrosus (AF) injuries can lead to substantial deterioration of intervertebral disc (IVD) which characterizes degenerative disc disease (DDD). However, treatments for AF repair/regeneration remain challenging due to the intrinsic heterogeneity of AF tissue at cellular, biochemical, and biomechanical levels. In this study, we isolated and characterized a sub-population of cells from rabbit AF tissue which formed colonies in vitro and could self-renew. These cells showed gene expression of typical surface antigen molecules characterizing mesenchymal stem cells (MSCs), including CD29, CD44, and CD166. Meanwhile, they did not express negative markers of MSCs such as CD4, CD8, and CD14. They also expressed Oct-4, nucleostemin, and SSEA-4 proteins. Upon induced differentiation they showed typical osteogenesis, chondrogenesis, and adipogenesis potential. Together, these AF-derived colony-forming cells possessed clonogenicity, self-renewal, and multi-potential differentiation capability, the three criteria characterizing MSCs. Such AF-derived stem cells may potentially be an ideal candidate for DDD treatments using cell therapies or tissue engineering approaches. Public Library of Science 2014-09-26 /pmc/articles/PMC4178129/ /pubmed/25259600 http://dx.doi.org/10.1371/journal.pone.0108239 Text en © 2014 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Liu, Chen Guo, Qianping Li, Jun Wang, Shenghao Wang, Yibin Li, Bin Yang, Huilin Identification of Rabbit Annulus Fibrosus-Derived Stem Cells |
title | Identification of Rabbit Annulus Fibrosus-Derived Stem Cells |
title_full | Identification of Rabbit Annulus Fibrosus-Derived Stem Cells |
title_fullStr | Identification of Rabbit Annulus Fibrosus-Derived Stem Cells |
title_full_unstemmed | Identification of Rabbit Annulus Fibrosus-Derived Stem Cells |
title_short | Identification of Rabbit Annulus Fibrosus-Derived Stem Cells |
title_sort | identification of rabbit annulus fibrosus-derived stem cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178129/ https://www.ncbi.nlm.nih.gov/pubmed/25259600 http://dx.doi.org/10.1371/journal.pone.0108239 |
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