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Identification of Rabbit Annulus Fibrosus-Derived Stem Cells

Annulus fibrosus (AF) injuries can lead to substantial deterioration of intervertebral disc (IVD) which characterizes degenerative disc disease (DDD). However, treatments for AF repair/regeneration remain challenging due to the intrinsic heterogeneity of AF tissue at cellular, biochemical, and biome...

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Detalles Bibliográficos
Autores principales: Liu, Chen, Guo, Qianping, Li, Jun, Wang, Shenghao, Wang, Yibin, Li, Bin, Yang, Huilin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178129/
https://www.ncbi.nlm.nih.gov/pubmed/25259600
http://dx.doi.org/10.1371/journal.pone.0108239
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author Liu, Chen
Guo, Qianping
Li, Jun
Wang, Shenghao
Wang, Yibin
Li, Bin
Yang, Huilin
author_facet Liu, Chen
Guo, Qianping
Li, Jun
Wang, Shenghao
Wang, Yibin
Li, Bin
Yang, Huilin
author_sort Liu, Chen
collection PubMed
description Annulus fibrosus (AF) injuries can lead to substantial deterioration of intervertebral disc (IVD) which characterizes degenerative disc disease (DDD). However, treatments for AF repair/regeneration remain challenging due to the intrinsic heterogeneity of AF tissue at cellular, biochemical, and biomechanical levels. In this study, we isolated and characterized a sub-population of cells from rabbit AF tissue which formed colonies in vitro and could self-renew. These cells showed gene expression of typical surface antigen molecules characterizing mesenchymal stem cells (MSCs), including CD29, CD44, and CD166. Meanwhile, they did not express negative markers of MSCs such as CD4, CD8, and CD14. They also expressed Oct-4, nucleostemin, and SSEA-4 proteins. Upon induced differentiation they showed typical osteogenesis, chondrogenesis, and adipogenesis potential. Together, these AF-derived colony-forming cells possessed clonogenicity, self-renewal, and multi-potential differentiation capability, the three criteria characterizing MSCs. Such AF-derived stem cells may potentially be an ideal candidate for DDD treatments using cell therapies or tissue engineering approaches.
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spelling pubmed-41781292014-10-02 Identification of Rabbit Annulus Fibrosus-Derived Stem Cells Liu, Chen Guo, Qianping Li, Jun Wang, Shenghao Wang, Yibin Li, Bin Yang, Huilin PLoS One Research Article Annulus fibrosus (AF) injuries can lead to substantial deterioration of intervertebral disc (IVD) which characterizes degenerative disc disease (DDD). However, treatments for AF repair/regeneration remain challenging due to the intrinsic heterogeneity of AF tissue at cellular, biochemical, and biomechanical levels. In this study, we isolated and characterized a sub-population of cells from rabbit AF tissue which formed colonies in vitro and could self-renew. These cells showed gene expression of typical surface antigen molecules characterizing mesenchymal stem cells (MSCs), including CD29, CD44, and CD166. Meanwhile, they did not express negative markers of MSCs such as CD4, CD8, and CD14. They also expressed Oct-4, nucleostemin, and SSEA-4 proteins. Upon induced differentiation they showed typical osteogenesis, chondrogenesis, and adipogenesis potential. Together, these AF-derived colony-forming cells possessed clonogenicity, self-renewal, and multi-potential differentiation capability, the three criteria characterizing MSCs. Such AF-derived stem cells may potentially be an ideal candidate for DDD treatments using cell therapies or tissue engineering approaches. Public Library of Science 2014-09-26 /pmc/articles/PMC4178129/ /pubmed/25259600 http://dx.doi.org/10.1371/journal.pone.0108239 Text en © 2014 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Liu, Chen
Guo, Qianping
Li, Jun
Wang, Shenghao
Wang, Yibin
Li, Bin
Yang, Huilin
Identification of Rabbit Annulus Fibrosus-Derived Stem Cells
title Identification of Rabbit Annulus Fibrosus-Derived Stem Cells
title_full Identification of Rabbit Annulus Fibrosus-Derived Stem Cells
title_fullStr Identification of Rabbit Annulus Fibrosus-Derived Stem Cells
title_full_unstemmed Identification of Rabbit Annulus Fibrosus-Derived Stem Cells
title_short Identification of Rabbit Annulus Fibrosus-Derived Stem Cells
title_sort identification of rabbit annulus fibrosus-derived stem cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178129/
https://www.ncbi.nlm.nih.gov/pubmed/25259600
http://dx.doi.org/10.1371/journal.pone.0108239
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