A reverse transcription loop-mediated isothermal amplification assay to rapidly diagnose foot-and-mouth disease virus C

A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to rapidly detect foot-and-mouth disease virus serotype C (FMDV C). By testing 10-fold serial dilutions of FMDV C samples, sensitivity of the FMDV C RT-LAMP was found to be 10 times higher than that of conve...

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Detalles Bibliográficos
Autores principales: Ding, Yao-zhong, Zhou, Jian-hua, Ma, Li-na, Qi, Yan-ni, Wei, Gang, Zhang, Jie, Zhang, Yong-guang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2014
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178144/
https://www.ncbi.nlm.nih.gov/pubmed/24690607
http://dx.doi.org/10.4142/jvs.2014.15.3.423
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author Ding, Yao-zhong
Zhou, Jian-hua
Ma, Li-na
Qi, Yan-ni
Wei, Gang
Zhang, Jie
Zhang, Yong-guang
author_facet Ding, Yao-zhong
Zhou, Jian-hua
Ma, Li-na
Qi, Yan-ni
Wei, Gang
Zhang, Jie
Zhang, Yong-guang
author_sort Ding, Yao-zhong
collection PubMed
description A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to rapidly detect foot-and-mouth disease virus serotype C (FMDV C). By testing 10-fold serial dilutions of FMDV C samples, sensitivity of the FMDV C RT-LAMP was found to be 10 times higher than that of conventional reverse transcription-PCR (RT-PCR). No cross-reactivity with A, Asia 1, or O FMDV or swine vesicular disease virus (SVDV) indicated that FMDV C RT-LAMP may be an exciting novel method for detecting FMDV C.
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spelling pubmed-41781442014-09-30 A reverse transcription loop-mediated isothermal amplification assay to rapidly diagnose foot-and-mouth disease virus C Ding, Yao-zhong Zhou, Jian-hua Ma, Li-na Qi, Yan-ni Wei, Gang Zhang, Jie Zhang, Yong-guang J Vet Sci Original Article A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to rapidly detect foot-and-mouth disease virus serotype C (FMDV C). By testing 10-fold serial dilutions of FMDV C samples, sensitivity of the FMDV C RT-LAMP was found to be 10 times higher than that of conventional reverse transcription-PCR (RT-PCR). No cross-reactivity with A, Asia 1, or O FMDV or swine vesicular disease virus (SVDV) indicated that FMDV C RT-LAMP may be an exciting novel method for detecting FMDV C. The Korean Society of Veterinary Science 2014-09 2014-09-16 /pmc/articles/PMC4178144/ /pubmed/24690607 http://dx.doi.org/10.4142/jvs.2014.15.3.423 Text en © 2014 The Korean Society of Veterinary Science. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Ding, Yao-zhong
Zhou, Jian-hua
Ma, Li-na
Qi, Yan-ni
Wei, Gang
Zhang, Jie
Zhang, Yong-guang
A reverse transcription loop-mediated isothermal amplification assay to rapidly diagnose foot-and-mouth disease virus C
title A reverse transcription loop-mediated isothermal amplification assay to rapidly diagnose foot-and-mouth disease virus C
title_full A reverse transcription loop-mediated isothermal amplification assay to rapidly diagnose foot-and-mouth disease virus C
title_fullStr A reverse transcription loop-mediated isothermal amplification assay to rapidly diagnose foot-and-mouth disease virus C
title_full_unstemmed A reverse transcription loop-mediated isothermal amplification assay to rapidly diagnose foot-and-mouth disease virus C
title_short A reverse transcription loop-mediated isothermal amplification assay to rapidly diagnose foot-and-mouth disease virus C
title_sort reverse transcription loop-mediated isothermal amplification assay to rapidly diagnose foot-and-mouth disease virus c
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178144/
https://www.ncbi.nlm.nih.gov/pubmed/24690607
http://dx.doi.org/10.4142/jvs.2014.15.3.423
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