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Quantitative measurement of influenza virus replication using consecutive bronchoalveolar lavage in the lower respiratory tract of a ferret model

The ferret is an established animal model of influenza virus infection. Although viral replication in the upper respiratory tract is usually measured with consecutively collected nasal washes, daily evaluation of viral replication in the lung is limited because a large numbers of ferrets need to be...

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Autores principales: Lee, Dong-Hun, Kim, Jong-In, Lee, Jae-Won, Chung, Wook-Hun, Park, Jae-Keun, Lee, Yu-Na, Han, Jin Soo, Kim, Hwi-Yool, Lee, Sang-Won, Song, Chang-Seon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178147/
https://www.ncbi.nlm.nih.gov/pubmed/24690606
http://dx.doi.org/10.4142/jvs.2014.15.3.439
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author Lee, Dong-Hun
Kim, Jong-In
Lee, Jae-Won
Chung, Wook-Hun
Park, Jae-Keun
Lee, Yu-Na
Han, Jin Soo
Kim, Hwi-Yool
Lee, Sang-Won
Song, Chang-Seon
author_facet Lee, Dong-Hun
Kim, Jong-In
Lee, Jae-Won
Chung, Wook-Hun
Park, Jae-Keun
Lee, Yu-Na
Han, Jin Soo
Kim, Hwi-Yool
Lee, Sang-Won
Song, Chang-Seon
author_sort Lee, Dong-Hun
collection PubMed
description The ferret is an established animal model of influenza virus infection. Although viral replication in the upper respiratory tract is usually measured with consecutively collected nasal washes, daily evaluation of viral replication in the lung is limited because a large numbers of ferrets need to be sacrificed at consecutive time points. To overcome this limitation, we performed a virus quantification assay using bronchoalveolar lavage (BAL) fluid. This non-invasive BAL technique allows consecutive quantification of virus replication in the lungs of living ferrets. Our method can be used for the longitudinal evaluation of virus tropism in the lower respiratory tract.
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spelling pubmed-41781472014-09-30 Quantitative measurement of influenza virus replication using consecutive bronchoalveolar lavage in the lower respiratory tract of a ferret model Lee, Dong-Hun Kim, Jong-In Lee, Jae-Won Chung, Wook-Hun Park, Jae-Keun Lee, Yu-Na Han, Jin Soo Kim, Hwi-Yool Lee, Sang-Won Song, Chang-Seon J Vet Sci Short Communication The ferret is an established animal model of influenza virus infection. Although viral replication in the upper respiratory tract is usually measured with consecutively collected nasal washes, daily evaluation of viral replication in the lung is limited because a large numbers of ferrets need to be sacrificed at consecutive time points. To overcome this limitation, we performed a virus quantification assay using bronchoalveolar lavage (BAL) fluid. This non-invasive BAL technique allows consecutive quantification of virus replication in the lungs of living ferrets. Our method can be used for the longitudinal evaluation of virus tropism in the lower respiratory tract. The Korean Society of Veterinary Science 2014-09 2014-09-16 /pmc/articles/PMC4178147/ /pubmed/24690606 http://dx.doi.org/10.4142/jvs.2014.15.3.439 Text en © 2014 The Korean Society of Veterinary Science. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Lee, Dong-Hun
Kim, Jong-In
Lee, Jae-Won
Chung, Wook-Hun
Park, Jae-Keun
Lee, Yu-Na
Han, Jin Soo
Kim, Hwi-Yool
Lee, Sang-Won
Song, Chang-Seon
Quantitative measurement of influenza virus replication using consecutive bronchoalveolar lavage in the lower respiratory tract of a ferret model
title Quantitative measurement of influenza virus replication using consecutive bronchoalveolar lavage in the lower respiratory tract of a ferret model
title_full Quantitative measurement of influenza virus replication using consecutive bronchoalveolar lavage in the lower respiratory tract of a ferret model
title_fullStr Quantitative measurement of influenza virus replication using consecutive bronchoalveolar lavage in the lower respiratory tract of a ferret model
title_full_unstemmed Quantitative measurement of influenza virus replication using consecutive bronchoalveolar lavage in the lower respiratory tract of a ferret model
title_short Quantitative measurement of influenza virus replication using consecutive bronchoalveolar lavage in the lower respiratory tract of a ferret model
title_sort quantitative measurement of influenza virus replication using consecutive bronchoalveolar lavage in the lower respiratory tract of a ferret model
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178147/
https://www.ncbi.nlm.nih.gov/pubmed/24690606
http://dx.doi.org/10.4142/jvs.2014.15.3.439
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