Re-Evaluation of the Critical Concentration for Ethambutol Antimicrobial Sensitivity Testing on the MGIT 960

The critical concentration (CC) for ethambutol testing on the Bactec MGIT 960 M. tuberculosis susceptibility testing has been questioned in recent publications. In this study, we correlate susceptibility results from the Bactec 460, MGIT 960 and embB gene sequencing to determine if the Bactec MGIT 9...

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Autores principales: Christianson, Sara, Voth, Dylan, Wolfe, Joyce, Sharma, Meenu K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178229/
https://www.ncbi.nlm.nih.gov/pubmed/25259734
http://dx.doi.org/10.1371/journal.pone.0108911
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author Christianson, Sara
Voth, Dylan
Wolfe, Joyce
Sharma, Meenu K.
author_facet Christianson, Sara
Voth, Dylan
Wolfe, Joyce
Sharma, Meenu K.
author_sort Christianson, Sara
collection PubMed
description The critical concentration (CC) for ethambutol testing on the Bactec MGIT 960 M. tuberculosis susceptibility testing has been questioned in recent publications. In this study, we correlate susceptibility results from the Bactec 460, MGIT 960 and embB gene sequencing to determine if the Bactec MGIT 960 adequately detects ethambutol resistance. We discovered discrepancies between the methods that highlight a need to re-evaluate ethambutol susceptibility testing recommendations, namely by considering lowering currently recommended CC on the MGIT 960. Further studies on the clinical significance of low-level ethambutol resistance are also required.
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spelling pubmed-41782292014-10-02 Re-Evaluation of the Critical Concentration for Ethambutol Antimicrobial Sensitivity Testing on the MGIT 960 Christianson, Sara Voth, Dylan Wolfe, Joyce Sharma, Meenu K. PLoS One Research Article The critical concentration (CC) for ethambutol testing on the Bactec MGIT 960 M. tuberculosis susceptibility testing has been questioned in recent publications. In this study, we correlate susceptibility results from the Bactec 460, MGIT 960 and embB gene sequencing to determine if the Bactec MGIT 960 adequately detects ethambutol resistance. We discovered discrepancies between the methods that highlight a need to re-evaluate ethambutol susceptibility testing recommendations, namely by considering lowering currently recommended CC on the MGIT 960. Further studies on the clinical significance of low-level ethambutol resistance are also required. Public Library of Science 2014-09-26 /pmc/articles/PMC4178229/ /pubmed/25259734 http://dx.doi.org/10.1371/journal.pone.0108911 Text en © 2014 Christianson et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Christianson, Sara
Voth, Dylan
Wolfe, Joyce
Sharma, Meenu K.
Re-Evaluation of the Critical Concentration for Ethambutol Antimicrobial Sensitivity Testing on the MGIT 960
title Re-Evaluation of the Critical Concentration for Ethambutol Antimicrobial Sensitivity Testing on the MGIT 960
title_full Re-Evaluation of the Critical Concentration for Ethambutol Antimicrobial Sensitivity Testing on the MGIT 960
title_fullStr Re-Evaluation of the Critical Concentration for Ethambutol Antimicrobial Sensitivity Testing on the MGIT 960
title_full_unstemmed Re-Evaluation of the Critical Concentration for Ethambutol Antimicrobial Sensitivity Testing on the MGIT 960
title_short Re-Evaluation of the Critical Concentration for Ethambutol Antimicrobial Sensitivity Testing on the MGIT 960
title_sort re-evaluation of the critical concentration for ethambutol antimicrobial sensitivity testing on the mgit 960
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178229/
https://www.ncbi.nlm.nih.gov/pubmed/25259734
http://dx.doi.org/10.1371/journal.pone.0108911
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