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Lab-on-Chip Cytometry Based on Magnetoresistive Sensors for Bacteria Detection in Milk

Flow cytometers have been optimized for use in portable platforms, where cell separation, identification and counting can be achieved in a compact and modular format. This feature can be combined with magnetic detection, where magnetoresistive sensors can be integrated within microfluidic channels t...

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Detalles Bibliográficos
Autores principales: Fernandes, Ana C., Duarte, Carla M., Cardoso, Filipe A., Bexiga, Ricardo., Cardoso, Susana., Freitas, Paulo P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4179045/
https://www.ncbi.nlm.nih.gov/pubmed/25196163
http://dx.doi.org/10.3390/s140815496
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author Fernandes, Ana C.
Duarte, Carla M.
Cardoso, Filipe A.
Bexiga, Ricardo.
Cardoso, Susana.
Freitas, Paulo P.
author_facet Fernandes, Ana C.
Duarte, Carla M.
Cardoso, Filipe A.
Bexiga, Ricardo.
Cardoso, Susana.
Freitas, Paulo P.
author_sort Fernandes, Ana C.
collection PubMed
description Flow cytometers have been optimized for use in portable platforms, where cell separation, identification and counting can be achieved in a compact and modular format. This feature can be combined with magnetic detection, where magnetoresistive sensors can be integrated within microfluidic channels to detect magnetically labelled cells. This work describes a platform for in-flow detection of magnetically labelled cells with a magneto-resistive based cell cytometer. In particular, we present an example for the validation of the platform as a magnetic counter that identifies and quantifies Streptococcus agalactiae in milk.
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spelling pubmed-41790452014-10-02 Lab-on-Chip Cytometry Based on Magnetoresistive Sensors for Bacteria Detection in Milk Fernandes, Ana C. Duarte, Carla M. Cardoso, Filipe A. Bexiga, Ricardo. Cardoso, Susana. Freitas, Paulo P. Sensors (Basel) Article Flow cytometers have been optimized for use in portable platforms, where cell separation, identification and counting can be achieved in a compact and modular format. This feature can be combined with magnetic detection, where magnetoresistive sensors can be integrated within microfluidic channels to detect magnetically labelled cells. This work describes a platform for in-flow detection of magnetically labelled cells with a magneto-resistive based cell cytometer. In particular, we present an example for the validation of the platform as a magnetic counter that identifies and quantifies Streptococcus agalactiae in milk. MDPI 2014-08-21 /pmc/articles/PMC4179045/ /pubmed/25196163 http://dx.doi.org/10.3390/s140815496 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Fernandes, Ana C.
Duarte, Carla M.
Cardoso, Filipe A.
Bexiga, Ricardo.
Cardoso, Susana.
Freitas, Paulo P.
Lab-on-Chip Cytometry Based on Magnetoresistive Sensors for Bacteria Detection in Milk
title Lab-on-Chip Cytometry Based on Magnetoresistive Sensors for Bacteria Detection in Milk
title_full Lab-on-Chip Cytometry Based on Magnetoresistive Sensors for Bacteria Detection in Milk
title_fullStr Lab-on-Chip Cytometry Based on Magnetoresistive Sensors for Bacteria Detection in Milk
title_full_unstemmed Lab-on-Chip Cytometry Based on Magnetoresistive Sensors for Bacteria Detection in Milk
title_short Lab-on-Chip Cytometry Based on Magnetoresistive Sensors for Bacteria Detection in Milk
title_sort lab-on-chip cytometry based on magnetoresistive sensors for bacteria detection in milk
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4179045/
https://www.ncbi.nlm.nih.gov/pubmed/25196163
http://dx.doi.org/10.3390/s140815496
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