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Anopheles gambiae densovirus (AgDNV) has negligible effects on adult survival and transcriptome of its mosquito host
Mosquito densoviruses (DNVs) are candidate agents for paratransgenic control of malaria and other vector-borne diseases. Unlike other mosquito DNVs, the Anopheles gambiae DNV (AgDNV) is non-pathogenic to larval mosquitoes. However, the cost of infection upon adults and the molecular mechanisms under...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4179393/ https://www.ncbi.nlm.nih.gov/pubmed/25279264 http://dx.doi.org/10.7717/peerj.584 |
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author | Ren, Xiaoxia Hughes, Grant L. Niu, Guodong Suzuki, Yasutsugu Rasgon, Jason L. |
author_facet | Ren, Xiaoxia Hughes, Grant L. Niu, Guodong Suzuki, Yasutsugu Rasgon, Jason L. |
author_sort | Ren, Xiaoxia |
collection | PubMed |
description | Mosquito densoviruses (DNVs) are candidate agents for paratransgenic control of malaria and other vector-borne diseases. Unlike other mosquito DNVs, the Anopheles gambiae DNV (AgDNV) is non-pathogenic to larval mosquitoes. However, the cost of infection upon adults and the molecular mechanisms underpinning infection in the mosquito host are unknown. Using life table analysis, we show that AgDNV infection has minimal effects on An. gambiae survival (no significant effect in 2 replicates and a slight 2 day survival decrease in the third replicate). Using microarrays, we show that AgDNV has very minimal effect on the adult mosquito transcriptome, with only 4–15 genes differentially regulated depending on the statistical criteria imposed. The minimal impact upon global transcription provides some mechanistic understanding of lack of virus pathogenicity, suggesting a long co-evolutionary history that has shifted towards avirulence. From an applied standpoint, lack of strong induced fitness costs makes AgDNV an attractive agent for paratransgenic malaria control. |
format | Online Article Text |
id | pubmed-4179393 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-41793932014-10-02 Anopheles gambiae densovirus (AgDNV) has negligible effects on adult survival and transcriptome of its mosquito host Ren, Xiaoxia Hughes, Grant L. Niu, Guodong Suzuki, Yasutsugu Rasgon, Jason L. PeerJ Entomology Mosquito densoviruses (DNVs) are candidate agents for paratransgenic control of malaria and other vector-borne diseases. Unlike other mosquito DNVs, the Anopheles gambiae DNV (AgDNV) is non-pathogenic to larval mosquitoes. However, the cost of infection upon adults and the molecular mechanisms underpinning infection in the mosquito host are unknown. Using life table analysis, we show that AgDNV infection has minimal effects on An. gambiae survival (no significant effect in 2 replicates and a slight 2 day survival decrease in the third replicate). Using microarrays, we show that AgDNV has very minimal effect on the adult mosquito transcriptome, with only 4–15 genes differentially regulated depending on the statistical criteria imposed. The minimal impact upon global transcription provides some mechanistic understanding of lack of virus pathogenicity, suggesting a long co-evolutionary history that has shifted towards avirulence. From an applied standpoint, lack of strong induced fitness costs makes AgDNV an attractive agent for paratransgenic malaria control. PeerJ Inc. 2014-09-18 /pmc/articles/PMC4179393/ /pubmed/25279264 http://dx.doi.org/10.7717/peerj.584 Text en © 2014 Ren et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Entomology Ren, Xiaoxia Hughes, Grant L. Niu, Guodong Suzuki, Yasutsugu Rasgon, Jason L. Anopheles gambiae densovirus (AgDNV) has negligible effects on adult survival and transcriptome of its mosquito host |
title | Anopheles gambiae densovirus (AgDNV) has negligible effects on adult survival and transcriptome of its mosquito host |
title_full | Anopheles gambiae densovirus (AgDNV) has negligible effects on adult survival and transcriptome of its mosquito host |
title_fullStr | Anopheles gambiae densovirus (AgDNV) has negligible effects on adult survival and transcriptome of its mosquito host |
title_full_unstemmed | Anopheles gambiae densovirus (AgDNV) has negligible effects on adult survival and transcriptome of its mosquito host |
title_short | Anopheles gambiae densovirus (AgDNV) has negligible effects on adult survival and transcriptome of its mosquito host |
title_sort | anopheles gambiae densovirus (agdnv) has negligible effects on adult survival and transcriptome of its mosquito host |
topic | Entomology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4179393/ https://www.ncbi.nlm.nih.gov/pubmed/25279264 http://dx.doi.org/10.7717/peerj.584 |
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