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Antipruritic effect of cold stimulation at the Quchi Acupoint (LI11) in Mice
BACKGROUND: Acupuncture and moxibustion are used to treat pruritus and atopic dermatitis. However, whether cold stimulation (defined as that the temperature conducted under skin temperature) of acupoints affects itching in experimental murine models remains unclear. METHODS: The present study was de...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4179855/ https://www.ncbi.nlm.nih.gov/pubmed/25239797 http://dx.doi.org/10.1186/1472-6882-14-341 |
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author | Tsai, Kao-Sung Chen, Yung-Hsiang Chen, Huey-Yi Shen, Ein-Yiao Lee, Yu-Chen Shen, Jui-Lung Wu, San-Yuan Lin, Jaung-Geng Chen, Yi-Hung Chen, Wen-Chi |
author_facet | Tsai, Kao-Sung Chen, Yung-Hsiang Chen, Huey-Yi Shen, Ein-Yiao Lee, Yu-Chen Shen, Jui-Lung Wu, San-Yuan Lin, Jaung-Geng Chen, Yi-Hung Chen, Wen-Chi |
author_sort | Tsai, Kao-Sung |
collection | PubMed |
description | BACKGROUND: Acupuncture and moxibustion are used to treat pruritus and atopic dermatitis. However, whether cold stimulation (defined as that the temperature conducted under skin temperature) of acupoints affects itching in experimental murine models remains unclear. METHODS: The present study was designed to determine the therapeutic effects of different thermal stimulations at the Quchi acupoint (LI11) in a murine model in which scratching behaviour was elicited by subcutaneous injection with a pruritogenic agent (compound 48/80). Male ICR mice were divided into several groups as follows: control (saline), those receiving compound 48/80 and compound 48/80 with various thermal stimulations (5°C–45°C) at LI11 (n = 6 per group). The scratch response of each animal to these stimulations was recorded for 30 min. The antipruritic effect of the acupoint was further evaluated in LI11 and sham (non-acupoint) groups (n = 6 per group). RESULTS: Treatment with lower temperature (20°C) at the LI11 acupoint significantly attenuated compound 48/80-induced scratching; however, this antipruritic effect was not observed with stimulation at the sham point. The expression of c-fos in the neuron of the cervical spine induced by compound 48/80 was suppressed by cold stimulation at LI11. The antipruritic effect of cold stimulation was blocked by ruthium red (RR), a non-selective transient receptor potential (TRP) channel blocker, suggesting that TRP channels may play an important role in the antipruritic effect of cold stimulation at LI11 in mice. CONCLUSIONS: This study demonstrated that cold stimulation at LI11 attenuated compound 48/80-induced scratching behaviour in mice, possibly by a TRP-related pathway. |
format | Online Article Text |
id | pubmed-4179855 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-41798552014-10-01 Antipruritic effect of cold stimulation at the Quchi Acupoint (LI11) in Mice Tsai, Kao-Sung Chen, Yung-Hsiang Chen, Huey-Yi Shen, Ein-Yiao Lee, Yu-Chen Shen, Jui-Lung Wu, San-Yuan Lin, Jaung-Geng Chen, Yi-Hung Chen, Wen-Chi BMC Complement Altern Med Research Article BACKGROUND: Acupuncture and moxibustion are used to treat pruritus and atopic dermatitis. However, whether cold stimulation (defined as that the temperature conducted under skin temperature) of acupoints affects itching in experimental murine models remains unclear. METHODS: The present study was designed to determine the therapeutic effects of different thermal stimulations at the Quchi acupoint (LI11) in a murine model in which scratching behaviour was elicited by subcutaneous injection with a pruritogenic agent (compound 48/80). Male ICR mice were divided into several groups as follows: control (saline), those receiving compound 48/80 and compound 48/80 with various thermal stimulations (5°C–45°C) at LI11 (n = 6 per group). The scratch response of each animal to these stimulations was recorded for 30 min. The antipruritic effect of the acupoint was further evaluated in LI11 and sham (non-acupoint) groups (n = 6 per group). RESULTS: Treatment with lower temperature (20°C) at the LI11 acupoint significantly attenuated compound 48/80-induced scratching; however, this antipruritic effect was not observed with stimulation at the sham point. The expression of c-fos in the neuron of the cervical spine induced by compound 48/80 was suppressed by cold stimulation at LI11. The antipruritic effect of cold stimulation was blocked by ruthium red (RR), a non-selective transient receptor potential (TRP) channel blocker, suggesting that TRP channels may play an important role in the antipruritic effect of cold stimulation at LI11 in mice. CONCLUSIONS: This study demonstrated that cold stimulation at LI11 attenuated compound 48/80-induced scratching behaviour in mice, possibly by a TRP-related pathway. BioMed Central 2014-09-19 /pmc/articles/PMC4179855/ /pubmed/25239797 http://dx.doi.org/10.1186/1472-6882-14-341 Text en © Tsai et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Tsai, Kao-Sung Chen, Yung-Hsiang Chen, Huey-Yi Shen, Ein-Yiao Lee, Yu-Chen Shen, Jui-Lung Wu, San-Yuan Lin, Jaung-Geng Chen, Yi-Hung Chen, Wen-Chi Antipruritic effect of cold stimulation at the Quchi Acupoint (LI11) in Mice |
title | Antipruritic effect of cold stimulation at the Quchi Acupoint (LI11) in Mice |
title_full | Antipruritic effect of cold stimulation at the Quchi Acupoint (LI11) in Mice |
title_fullStr | Antipruritic effect of cold stimulation at the Quchi Acupoint (LI11) in Mice |
title_full_unstemmed | Antipruritic effect of cold stimulation at the Quchi Acupoint (LI11) in Mice |
title_short | Antipruritic effect of cold stimulation at the Quchi Acupoint (LI11) in Mice |
title_sort | antipruritic effect of cold stimulation at the quchi acupoint (li11) in mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4179855/ https://www.ncbi.nlm.nih.gov/pubmed/25239797 http://dx.doi.org/10.1186/1472-6882-14-341 |
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