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Anti-angiogenic potential of an ethanol extract of Annona atemoya seeds in vitro and in vivo
BACKGROUND: Angiogenesis, which is initiated by certain tumor micro-environmental conditions and diverse protein factors, plays a pivotal role during tumor development and metastasis. Therefore, many efforts have been made to develop effective anti-angiogenic agents as anticancer therapeutics. In th...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4180132/ https://www.ncbi.nlm.nih.gov/pubmed/25249371 http://dx.doi.org/10.1186/1472-6882-14-353 |
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author | Yi, Jin-Mu Park, Jong-Shik Lee, Jun Hong, Jin Tae Bang, Ok-Sun Kim, No Soo |
author_facet | Yi, Jin-Mu Park, Jong-Shik Lee, Jun Hong, Jin Tae Bang, Ok-Sun Kim, No Soo |
author_sort | Yi, Jin-Mu |
collection | PubMed |
description | BACKGROUND: Angiogenesis, which is initiated by certain tumor micro-environmental conditions and diverse protein factors, plays a pivotal role during tumor development and metastasis. Therefore, many efforts have been made to develop effective anti-angiogenic agents as anticancer therapeutics. In the current study, we investigated the anti-angiogenic potential of an ethanol extract of Annona atemoya seeds (EEAA) in vitro and in vivo. METHODS: The anti-angiogenic potential of EEAA was evaluated using various in vitro/in vivo models, including cell proliferation, migration, and tube formation by human umbilical vascular endothelial cells (HUVECs); a Matrigel plug assay; and tumor-induced angiogenesis. The expression of hypoxia-inducible factors (HIFs) and vascular endothelial growth factor (VEGF) was investigated using reverse transcription-polymerase chain reaction, immunoassays, and western blotting. RESULTS: EEAA was able to significantly inhibit the angiogenic properties of HUVECs in vitro as well as angiogenic factor-induced blood vessel formation in vivo. EEAA down-regulated the expression of VEGF and HIF-1alpha/2alpha at the mRNA and protein levels, respectively, in cancer cells under hypoxic conditions. CONCLUSIONS: EEAA shows a strong anti-angiogenic potential in both in vitro and in vivo systems, and we suggest that EEAA may be a valuable herbal source for anticancer drug development. |
format | Online Article Text |
id | pubmed-4180132 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-41801322014-10-01 Anti-angiogenic potential of an ethanol extract of Annona atemoya seeds in vitro and in vivo Yi, Jin-Mu Park, Jong-Shik Lee, Jun Hong, Jin Tae Bang, Ok-Sun Kim, No Soo BMC Complement Altern Med Research Article BACKGROUND: Angiogenesis, which is initiated by certain tumor micro-environmental conditions and diverse protein factors, plays a pivotal role during tumor development and metastasis. Therefore, many efforts have been made to develop effective anti-angiogenic agents as anticancer therapeutics. In the current study, we investigated the anti-angiogenic potential of an ethanol extract of Annona atemoya seeds (EEAA) in vitro and in vivo. METHODS: The anti-angiogenic potential of EEAA was evaluated using various in vitro/in vivo models, including cell proliferation, migration, and tube formation by human umbilical vascular endothelial cells (HUVECs); a Matrigel plug assay; and tumor-induced angiogenesis. The expression of hypoxia-inducible factors (HIFs) and vascular endothelial growth factor (VEGF) was investigated using reverse transcription-polymerase chain reaction, immunoassays, and western blotting. RESULTS: EEAA was able to significantly inhibit the angiogenic properties of HUVECs in vitro as well as angiogenic factor-induced blood vessel formation in vivo. EEAA down-regulated the expression of VEGF and HIF-1alpha/2alpha at the mRNA and protein levels, respectively, in cancer cells under hypoxic conditions. CONCLUSIONS: EEAA shows a strong anti-angiogenic potential in both in vitro and in vivo systems, and we suggest that EEAA may be a valuable herbal source for anticancer drug development. BioMed Central 2014-09-23 /pmc/articles/PMC4180132/ /pubmed/25249371 http://dx.doi.org/10.1186/1472-6882-14-353 Text en © Yi et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Yi, Jin-Mu Park, Jong-Shik Lee, Jun Hong, Jin Tae Bang, Ok-Sun Kim, No Soo Anti-angiogenic potential of an ethanol extract of Annona atemoya seeds in vitro and in vivo |
title | Anti-angiogenic potential of an ethanol extract of Annona atemoya seeds in vitro and in vivo |
title_full | Anti-angiogenic potential of an ethanol extract of Annona atemoya seeds in vitro and in vivo |
title_fullStr | Anti-angiogenic potential of an ethanol extract of Annona atemoya seeds in vitro and in vivo |
title_full_unstemmed | Anti-angiogenic potential of an ethanol extract of Annona atemoya seeds in vitro and in vivo |
title_short | Anti-angiogenic potential of an ethanol extract of Annona atemoya seeds in vitro and in vivo |
title_sort | anti-angiogenic potential of an ethanol extract of annona atemoya seeds in vitro and in vivo |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4180132/ https://www.ncbi.nlm.nih.gov/pubmed/25249371 http://dx.doi.org/10.1186/1472-6882-14-353 |
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