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Gradated assembly of multiple proteins into supramolecular nanomaterials

Biomaterials displaying precise ratios of different bioactive protein components are critical for applications ranging from vaccines to regenerative medicine, but their design is often hindered by limited choices and cross-reactivity of protein conjugation chemistries. Here, we describe a strategy f...

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Detalles Bibliográficos
Autores principales: Hudalla, Gregory A., Sun, Tao, Gasiorowski, Joshua Z., Han, Huifang, Tian, Ye F., Chong, Anita. S., Collier, Joel H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4180598/
https://www.ncbi.nlm.nih.gov/pubmed/24930032
http://dx.doi.org/10.1038/nmat3998
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author Hudalla, Gregory A.
Sun, Tao
Gasiorowski, Joshua Z.
Han, Huifang
Tian, Ye F.
Chong, Anita. S.
Collier, Joel H.
author_facet Hudalla, Gregory A.
Sun, Tao
Gasiorowski, Joshua Z.
Han, Huifang
Tian, Ye F.
Chong, Anita. S.
Collier, Joel H.
author_sort Hudalla, Gregory A.
collection PubMed
description Biomaterials displaying precise ratios of different bioactive protein components are critical for applications ranging from vaccines to regenerative medicine, but their design is often hindered by limited choices and cross-reactivity of protein conjugation chemistries. Here, we describe a strategy for inducing multiple different expressed proteins of choice to assemble into nanofibers and gels with exceptional compositional control. The strategy employs novel “βTail” tags, which allow for good protein expression in bacteriological cultures, yet can be induced to co-assemble into nanomaterials when mixed with additional β-sheet fibrillizing peptides. Multiple different βTail fusion proteins could be inserted into peptide nanofibers alone or in combination at predictable, smoothly gradated concentrations, providing a simple yet versatile route to install precise combinations of proteins into nanomaterials. The technology is illustrated by achieving precisely targeted hues using mixtures of fluorescent proteins, by creating nanofibers bearing enzymatic activity, and by adjusting antigenic dominance in vaccines.
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spelling pubmed-41805982015-02-01 Gradated assembly of multiple proteins into supramolecular nanomaterials Hudalla, Gregory A. Sun, Tao Gasiorowski, Joshua Z. Han, Huifang Tian, Ye F. Chong, Anita. S. Collier, Joel H. Nat Mater Article Biomaterials displaying precise ratios of different bioactive protein components are critical for applications ranging from vaccines to regenerative medicine, but their design is often hindered by limited choices and cross-reactivity of protein conjugation chemistries. Here, we describe a strategy for inducing multiple different expressed proteins of choice to assemble into nanofibers and gels with exceptional compositional control. The strategy employs novel “βTail” tags, which allow for good protein expression in bacteriological cultures, yet can be induced to co-assemble into nanomaterials when mixed with additional β-sheet fibrillizing peptides. Multiple different βTail fusion proteins could be inserted into peptide nanofibers alone or in combination at predictable, smoothly gradated concentrations, providing a simple yet versatile route to install precise combinations of proteins into nanomaterials. The technology is illustrated by achieving precisely targeted hues using mixtures of fluorescent proteins, by creating nanofibers bearing enzymatic activity, and by adjusting antigenic dominance in vaccines. 2014-06-15 2014-08 /pmc/articles/PMC4180598/ /pubmed/24930032 http://dx.doi.org/10.1038/nmat3998 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Hudalla, Gregory A.
Sun, Tao
Gasiorowski, Joshua Z.
Han, Huifang
Tian, Ye F.
Chong, Anita. S.
Collier, Joel H.
Gradated assembly of multiple proteins into supramolecular nanomaterials
title Gradated assembly of multiple proteins into supramolecular nanomaterials
title_full Gradated assembly of multiple proteins into supramolecular nanomaterials
title_fullStr Gradated assembly of multiple proteins into supramolecular nanomaterials
title_full_unstemmed Gradated assembly of multiple proteins into supramolecular nanomaterials
title_short Gradated assembly of multiple proteins into supramolecular nanomaterials
title_sort gradated assembly of multiple proteins into supramolecular nanomaterials
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4180598/
https://www.ncbi.nlm.nih.gov/pubmed/24930032
http://dx.doi.org/10.1038/nmat3998
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