Dynamics of CRISPR Loci in Microevolutionary Process of Yersinia pestis Strains

The potential use of CRISPR loci genotyping to elucidate population dynamics and microevolution of 146 Yersinia pestis strains from different biovars and locations was investigated in this work. The majority of strains from the Orientalis biovar presented specific spacer arrays, allowing for the est...

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Detalles Bibliográficos
Autores principales: Barros, Maria Paloma S., França, Camila T., Lins, Rosanny Holanda F. B., Santos, Milena Danda V., Silva, Ednaldo J., Oliveira, Maria Betânia M., Silveira-Filho, Vladimir M., Rezende, Antônio M., Balbino, Valdir Q., Leal-Balbino, Tereza Cristina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4180756/
https://www.ncbi.nlm.nih.gov/pubmed/25265542
http://dx.doi.org/10.1371/journal.pone.0108353
Descripción
Sumario:The potential use of CRISPR loci genotyping to elucidate population dynamics and microevolution of 146 Yersinia pestis strains from different biovars and locations was investigated in this work. The majority of strains from the Orientalis biovar presented specific spacer arrays, allowing for the establishment of a CRISPR signature for their respective isolates. Twenty-one new spacers were found in the Y. pestis strains from plague foci in Brazil. Ninety-three (64%) strains were grouped in the G1 genotype, whereas the others were distributed in 35 genotypes. This study allowed observing a microevolutionary process in a group of Y. pestis isolated from Brazil. We also identified specific genotypes of Y. pestis that were important for the establishment of the bacteria in plague foci in Brazil. The data have provided supporting evidence for the diversity and dynamics of CRISPR loci present in the genome of Y. pestis strains from plague foci in Brazil.

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