Multiplexed aberration measurement for deep tissue imaging in vivo

We describe a multiplexed aberration measurement method that modulates the intensity or phase of light rays at multiple pupil segments in parallel to determine their phase gradients. Applicable to fluorescent-protein-labeled structures of arbitrary complexity, it allows us to obtain diffraction-limi...

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Detalles Bibliográficos
Autores principales: Wang, Chen, Liu, Rui, Milkie, Daniel E., Sun, Wenzhi, Tan, Zhongchao, Kerlin, Aaron, Chen, Tsai-Wen, Kim, Douglas S., Ji, Na
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4180771/
https://www.ncbi.nlm.nih.gov/pubmed/25128976
http://dx.doi.org/10.1038/nmeth.3068
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author Wang, Chen
Liu, Rui
Milkie, Daniel E.
Sun, Wenzhi
Tan, Zhongchao
Kerlin, Aaron
Chen, Tsai-Wen
Kim, Douglas S.
Ji, Na
author_facet Wang, Chen
Liu, Rui
Milkie, Daniel E.
Sun, Wenzhi
Tan, Zhongchao
Kerlin, Aaron
Chen, Tsai-Wen
Kim, Douglas S.
Ji, Na
author_sort Wang, Chen
collection PubMed
description We describe a multiplexed aberration measurement method that modulates the intensity or phase of light rays at multiple pupil segments in parallel to determine their phase gradients. Applicable to fluorescent-protein-labeled structures of arbitrary complexity, it allows us to obtain diffraction-limited resolution in various samples in vivo. For the strongly scattering mouse brain, a single aberration correction improves structural and functional imaging of fine neuronal processes over a large imaging volume.
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spelling pubmed-41807712015-04-01 Multiplexed aberration measurement for deep tissue imaging in vivo Wang, Chen Liu, Rui Milkie, Daniel E. Sun, Wenzhi Tan, Zhongchao Kerlin, Aaron Chen, Tsai-Wen Kim, Douglas S. Ji, Na Nat Methods Article We describe a multiplexed aberration measurement method that modulates the intensity or phase of light rays at multiple pupil segments in parallel to determine their phase gradients. Applicable to fluorescent-protein-labeled structures of arbitrary complexity, it allows us to obtain diffraction-limited resolution in various samples in vivo. For the strongly scattering mouse brain, a single aberration correction improves structural and functional imaging of fine neuronal processes over a large imaging volume. 2014-08-17 2014-10 /pmc/articles/PMC4180771/ /pubmed/25128976 http://dx.doi.org/10.1038/nmeth.3068 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Wang, Chen
Liu, Rui
Milkie, Daniel E.
Sun, Wenzhi
Tan, Zhongchao
Kerlin, Aaron
Chen, Tsai-Wen
Kim, Douglas S.
Ji, Na
Multiplexed aberration measurement for deep tissue imaging in vivo
title Multiplexed aberration measurement for deep tissue imaging in vivo
title_full Multiplexed aberration measurement for deep tissue imaging in vivo
title_fullStr Multiplexed aberration measurement for deep tissue imaging in vivo
title_full_unstemmed Multiplexed aberration measurement for deep tissue imaging in vivo
title_short Multiplexed aberration measurement for deep tissue imaging in vivo
title_sort multiplexed aberration measurement for deep tissue imaging in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4180771/
https://www.ncbi.nlm.nih.gov/pubmed/25128976
http://dx.doi.org/10.1038/nmeth.3068
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