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Systemic NK cell ablation attenuates intra-abdominal adipose tissue macrophage infiltration in murine obesity
OBJECTIVE: NK cells are understudied in the context of metabolic disease and obesity. The goal of this study was to define the effect of NK cell ablation on systemic inflammation and glucose homeostasis in murine obesity. METHODS: A transgenic murine model was used to study the effect of NK cell abl...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4180782/ https://www.ncbi.nlm.nih.gov/pubmed/24962029 http://dx.doi.org/10.1002/oby.20823 |
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author | O’Rourke, Robert W. Meyer, Kevin A. Neeley, Christopher K. Gaston, Garen D. Sekhri, Palak Szumowski, Marek Zamarron, Brian Lumeng, Carey N. Marks, Daniel L. |
author_facet | O’Rourke, Robert W. Meyer, Kevin A. Neeley, Christopher K. Gaston, Garen D. Sekhri, Palak Szumowski, Marek Zamarron, Brian Lumeng, Carey N. Marks, Daniel L. |
author_sort | O’Rourke, Robert W. |
collection | PubMed |
description | OBJECTIVE: NK cells are understudied in the context of metabolic disease and obesity. The goal of this study was to define the effect of NK cell ablation on systemic inflammation and glucose homeostasis in murine obesity. METHODS: A transgenic murine model was used to study the effect of NK cell ablation on systemic inflammation and glucose homeostasis in the context of diet-induced obesity using flow cytometry, QRTPCR, and glucose tolerance and insulin sensitivity testing. RESULTS: NK cell ablation achieved a 3-4 fold decrease in NK cells but had no effect on T-cell levels in adipose tissues and spleen. NK cell ablation was associated with decreased total macrophage infiltration in intra-abdominal adipose tissue, but macrophage infiltration in subcutaneous adipose tissue and spleen was unaffected. NK cell ablation was associated with modest improvement in insulin sensitivity but had no effect on tissue transcript levels of inflammatory cytokines. CONCLUSION: NK cells play a role in promoting intra-abdominal adipose tissue macrophage infiltration and systemic insulin resistance in obesity. |
format | Online Article Text |
id | pubmed-4180782 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
record_format | MEDLINE/PubMed |
spelling | pubmed-41807822015-10-01 Systemic NK cell ablation attenuates intra-abdominal adipose tissue macrophage infiltration in murine obesity O’Rourke, Robert W. Meyer, Kevin A. Neeley, Christopher K. Gaston, Garen D. Sekhri, Palak Szumowski, Marek Zamarron, Brian Lumeng, Carey N. Marks, Daniel L. Obesity (Silver Spring) Article OBJECTIVE: NK cells are understudied in the context of metabolic disease and obesity. The goal of this study was to define the effect of NK cell ablation on systemic inflammation and glucose homeostasis in murine obesity. METHODS: A transgenic murine model was used to study the effect of NK cell ablation on systemic inflammation and glucose homeostasis in the context of diet-induced obesity using flow cytometry, QRTPCR, and glucose tolerance and insulin sensitivity testing. RESULTS: NK cell ablation achieved a 3-4 fold decrease in NK cells but had no effect on T-cell levels in adipose tissues and spleen. NK cell ablation was associated with decreased total macrophage infiltration in intra-abdominal adipose tissue, but macrophage infiltration in subcutaneous adipose tissue and spleen was unaffected. NK cell ablation was associated with modest improvement in insulin sensitivity but had no effect on tissue transcript levels of inflammatory cytokines. CONCLUSION: NK cells play a role in promoting intra-abdominal adipose tissue macrophage infiltration and systemic insulin resistance in obesity. 2014-06-24 2014-10 /pmc/articles/PMC4180782/ /pubmed/24962029 http://dx.doi.org/10.1002/oby.20823 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article O’Rourke, Robert W. Meyer, Kevin A. Neeley, Christopher K. Gaston, Garen D. Sekhri, Palak Szumowski, Marek Zamarron, Brian Lumeng, Carey N. Marks, Daniel L. Systemic NK cell ablation attenuates intra-abdominal adipose tissue macrophage infiltration in murine obesity |
title | Systemic NK cell ablation attenuates intra-abdominal adipose tissue macrophage infiltration in murine obesity |
title_full | Systemic NK cell ablation attenuates intra-abdominal adipose tissue macrophage infiltration in murine obesity |
title_fullStr | Systemic NK cell ablation attenuates intra-abdominal adipose tissue macrophage infiltration in murine obesity |
title_full_unstemmed | Systemic NK cell ablation attenuates intra-abdominal adipose tissue macrophage infiltration in murine obesity |
title_short | Systemic NK cell ablation attenuates intra-abdominal adipose tissue macrophage infiltration in murine obesity |
title_sort | systemic nk cell ablation attenuates intra-abdominal adipose tissue macrophage infiltration in murine obesity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4180782/ https://www.ncbi.nlm.nih.gov/pubmed/24962029 http://dx.doi.org/10.1002/oby.20823 |
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