A peptide probe for the detection of neurokinin-1 receptor by disaggregation enhanced fluorescence and magnetic resonance signals

We report a novel peptide probe for the detection of neurokinin-1 receptor using disaggregation-caused signal enhancement. The probe was obtained via the aggregation of a modified substance P in a terpyridine-Fe (II) complex with Gd (III)-DOTA into well-defined nanostructures, which effectively weak...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Jingxian, Zou, Rongfeng, Wang, Qi, Xue, Yajing, Wei, Ping, Yang, Shiping, Wu, Junchen, Tian, He
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4180826/
https://www.ncbi.nlm.nih.gov/pubmed/25270511
http://dx.doi.org/10.1038/srep06487
_version_ 1782337266535890944
author Wu, Jingxian
Zou, Rongfeng
Wang, Qi
Xue, Yajing
Wei, Ping
Yang, Shiping
Wu, Junchen
Tian, He
author_facet Wu, Jingxian
Zou, Rongfeng
Wang, Qi
Xue, Yajing
Wei, Ping
Yang, Shiping
Wu, Junchen
Tian, He
author_sort Wu, Jingxian
collection PubMed
description We report a novel peptide probe for the detection of neurokinin-1 receptor using disaggregation-caused signal enhancement. The probe was obtained via the aggregation of a modified substance P in a terpyridine-Fe (II) complex with Gd (III)-DOTA into well-defined nanostructures, which effectively weaken ligand fluorescence and slow the exchange rate of inner-sphere water molecules. This probe disaggregates upon binding to the neurokinin-1 receptor and activates the contrast agents to generate a fluorescent signal that positively enhances magnetic resonance imaging contrast and allows for the detection of overexpressed receptors on tumor cells and the identification of lung cancer using serum samples.
format Online
Article
Text
id pubmed-4180826
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-41808262014-10-02 A peptide probe for the detection of neurokinin-1 receptor by disaggregation enhanced fluorescence and magnetic resonance signals Wu, Jingxian Zou, Rongfeng Wang, Qi Xue, Yajing Wei, Ping Yang, Shiping Wu, Junchen Tian, He Sci Rep Article We report a novel peptide probe for the detection of neurokinin-1 receptor using disaggregation-caused signal enhancement. The probe was obtained via the aggregation of a modified substance P in a terpyridine-Fe (II) complex with Gd (III)-DOTA into well-defined nanostructures, which effectively weaken ligand fluorescence and slow the exchange rate of inner-sphere water molecules. This probe disaggregates upon binding to the neurokinin-1 receptor and activates the contrast agents to generate a fluorescent signal that positively enhances magnetic resonance imaging contrast and allows for the detection of overexpressed receptors on tumor cells and the identification of lung cancer using serum samples. Nature Publishing Group 2014-10-01 /pmc/articles/PMC4180826/ /pubmed/25270511 http://dx.doi.org/10.1038/srep06487 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle Article
Wu, Jingxian
Zou, Rongfeng
Wang, Qi
Xue, Yajing
Wei, Ping
Yang, Shiping
Wu, Junchen
Tian, He
A peptide probe for the detection of neurokinin-1 receptor by disaggregation enhanced fluorescence and magnetic resonance signals
title A peptide probe for the detection of neurokinin-1 receptor by disaggregation enhanced fluorescence and magnetic resonance signals
title_full A peptide probe for the detection of neurokinin-1 receptor by disaggregation enhanced fluorescence and magnetic resonance signals
title_fullStr A peptide probe for the detection of neurokinin-1 receptor by disaggregation enhanced fluorescence and magnetic resonance signals
title_full_unstemmed A peptide probe for the detection of neurokinin-1 receptor by disaggregation enhanced fluorescence and magnetic resonance signals
title_short A peptide probe for the detection of neurokinin-1 receptor by disaggregation enhanced fluorescence and magnetic resonance signals
title_sort peptide probe for the detection of neurokinin-1 receptor by disaggregation enhanced fluorescence and magnetic resonance signals
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4180826/
https://www.ncbi.nlm.nih.gov/pubmed/25270511
http://dx.doi.org/10.1038/srep06487
work_keys_str_mv AT wujingxian apeptideprobeforthedetectionofneurokinin1receptorbydisaggregationenhancedfluorescenceandmagneticresonancesignals
AT zourongfeng apeptideprobeforthedetectionofneurokinin1receptorbydisaggregationenhancedfluorescenceandmagneticresonancesignals
AT wangqi apeptideprobeforthedetectionofneurokinin1receptorbydisaggregationenhancedfluorescenceandmagneticresonancesignals
AT xueyajing apeptideprobeforthedetectionofneurokinin1receptorbydisaggregationenhancedfluorescenceandmagneticresonancesignals
AT weiping apeptideprobeforthedetectionofneurokinin1receptorbydisaggregationenhancedfluorescenceandmagneticresonancesignals
AT yangshiping apeptideprobeforthedetectionofneurokinin1receptorbydisaggregationenhancedfluorescenceandmagneticresonancesignals
AT wujunchen apeptideprobeforthedetectionofneurokinin1receptorbydisaggregationenhancedfluorescenceandmagneticresonancesignals
AT tianhe apeptideprobeforthedetectionofneurokinin1receptorbydisaggregationenhancedfluorescenceandmagneticresonancesignals
AT wujingxian peptideprobeforthedetectionofneurokinin1receptorbydisaggregationenhancedfluorescenceandmagneticresonancesignals
AT zourongfeng peptideprobeforthedetectionofneurokinin1receptorbydisaggregationenhancedfluorescenceandmagneticresonancesignals
AT wangqi peptideprobeforthedetectionofneurokinin1receptorbydisaggregationenhancedfluorescenceandmagneticresonancesignals
AT xueyajing peptideprobeforthedetectionofneurokinin1receptorbydisaggregationenhancedfluorescenceandmagneticresonancesignals
AT weiping peptideprobeforthedetectionofneurokinin1receptorbydisaggregationenhancedfluorescenceandmagneticresonancesignals
AT yangshiping peptideprobeforthedetectionofneurokinin1receptorbydisaggregationenhancedfluorescenceandmagneticresonancesignals
AT wujunchen peptideprobeforthedetectionofneurokinin1receptorbydisaggregationenhancedfluorescenceandmagneticresonancesignals
AT tianhe peptideprobeforthedetectionofneurokinin1receptorbydisaggregationenhancedfluorescenceandmagneticresonancesignals

Ejemplares similares