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Majority of T. gondii seropositive chickens (Gallus domesticus) in Central Ethiopia carries the infective parasite

BACKGROUND: The prevalence of Toxoplasma gondii in free range chickens is a good indicator of the prevalence of T. gondii oocysts in the environment. The aim of this study was to isolate T. gondii parasites from heart and brain of seropositive free range (FR) chickens. FINDINGS: Isolation of T. gond...

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Autores principales: Gebremedhin, Endrias Zewdu, Tesfamaryam, Gebregergs, Duguma, Reta, Tilahun, Getachew, Di Marco, Vincenzo, Vitale, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4180848/
https://www.ncbi.nlm.nih.gov/pubmed/25248281
http://dx.doi.org/10.1186/s13028-014-0060-4
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author Gebremedhin, Endrias Zewdu
Tesfamaryam, Gebregergs
Duguma, Reta
Tilahun, Getachew
Di Marco, Vincenzo
Vitale, Maria
author_facet Gebremedhin, Endrias Zewdu
Tesfamaryam, Gebregergs
Duguma, Reta
Tilahun, Getachew
Di Marco, Vincenzo
Vitale, Maria
author_sort Gebremedhin, Endrias Zewdu
collection PubMed
description BACKGROUND: The prevalence of Toxoplasma gondii in free range chickens is a good indicator of the prevalence of T. gondii oocysts in the environment. The aim of this study was to isolate T. gondii parasites from heart and brain of seropositive free range (FR) chickens. FINDINGS: Isolation of T. gondii from pooled heart and brain of 41 direct agglutination test (DAT) positive (≥1:40) free range chickens (Gallus domesticus) was carried out by bioassay in mice. T. gondii specific antibodies in mice were assayed by DAT and microscopy was employed for detection and enumeration of brain tissue cysts. Overall, bioassay was positive in 29 (70.7%) chicken samples. T. gondii tissue cysts were isolated from 59% (24/41) of bioassayed chickens: from 2 of 7 chickens with a titer of 1: ≤ 60, 2 of 5 with titer 1: 180, 6 of 8 with titer 1: 540, 10 of 15 with titer 1: 1620, 1 of 2 with titer 1: 6000, 2 of 3 with titer 1:18000, 1 of 1 with titer 1:54000. None of the isolates was pathogenic for mice. Tissue cysts were detected from 61% of seropositive mice (DAT ≥ 1:40). Generally, tissue cyst counts per brain of mouse were low (mean: 132.7 ± 84.4; range: 47–352). CONCLUSIONS: Majority of T. gondii seropositive chickens (Gallus domesticus) in Central. Ethiopia carries the infective parasite. Tissues from the free range chicken might be a source infection for animals and humans.
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spelling pubmed-41808482014-10-03 Majority of T. gondii seropositive chickens (Gallus domesticus) in Central Ethiopia carries the infective parasite Gebremedhin, Endrias Zewdu Tesfamaryam, Gebregergs Duguma, Reta Tilahun, Getachew Di Marco, Vincenzo Vitale, Maria Acta Vet Scand Brief Communication BACKGROUND: The prevalence of Toxoplasma gondii in free range chickens is a good indicator of the prevalence of T. gondii oocysts in the environment. The aim of this study was to isolate T. gondii parasites from heart and brain of seropositive free range (FR) chickens. FINDINGS: Isolation of T. gondii from pooled heart and brain of 41 direct agglutination test (DAT) positive (≥1:40) free range chickens (Gallus domesticus) was carried out by bioassay in mice. T. gondii specific antibodies in mice were assayed by DAT and microscopy was employed for detection and enumeration of brain tissue cysts. Overall, bioassay was positive in 29 (70.7%) chicken samples. T. gondii tissue cysts were isolated from 59% (24/41) of bioassayed chickens: from 2 of 7 chickens with a titer of 1: ≤ 60, 2 of 5 with titer 1: 180, 6 of 8 with titer 1: 540, 10 of 15 with titer 1: 1620, 1 of 2 with titer 1: 6000, 2 of 3 with titer 1:18000, 1 of 1 with titer 1:54000. None of the isolates was pathogenic for mice. Tissue cysts were detected from 61% of seropositive mice (DAT ≥ 1:40). Generally, tissue cyst counts per brain of mouse were low (mean: 132.7 ± 84.4; range: 47–352). CONCLUSIONS: Majority of T. gondii seropositive chickens (Gallus domesticus) in Central. Ethiopia carries the infective parasite. Tissues from the free range chicken might be a source infection for animals and humans. BioMed Central 2014-09-24 /pmc/articles/PMC4180848/ /pubmed/25248281 http://dx.doi.org/10.1186/s13028-014-0060-4 Text en © Gebremedhin et al.; licensee BioMed Central Ltd. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Brief Communication
Gebremedhin, Endrias Zewdu
Tesfamaryam, Gebregergs
Duguma, Reta
Tilahun, Getachew
Di Marco, Vincenzo
Vitale, Maria
Majority of T. gondii seropositive chickens (Gallus domesticus) in Central Ethiopia carries the infective parasite
title Majority of T. gondii seropositive chickens (Gallus domesticus) in Central Ethiopia carries the infective parasite
title_full Majority of T. gondii seropositive chickens (Gallus domesticus) in Central Ethiopia carries the infective parasite
title_fullStr Majority of T. gondii seropositive chickens (Gallus domesticus) in Central Ethiopia carries the infective parasite
title_full_unstemmed Majority of T. gondii seropositive chickens (Gallus domesticus) in Central Ethiopia carries the infective parasite
title_short Majority of T. gondii seropositive chickens (Gallus domesticus) in Central Ethiopia carries the infective parasite
title_sort majority of t. gondii seropositive chickens (gallus domesticus) in central ethiopia carries the infective parasite
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4180848/
https://www.ncbi.nlm.nih.gov/pubmed/25248281
http://dx.doi.org/10.1186/s13028-014-0060-4
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