Cargando…
Trans-Golgi network localized small GTPase RabA1d is involved in cell plate formation and oscillatory root hair growth
BACKGROUND: Small Rab GTPases are important regulators of vesicular trafficking in plants. AtRabA1d, a member of the RabA1 subfamily of small GTPases, was previously found in the vesicle-rich apical dome of growing root hairs suggesting a role during tip growth; however, its specific intracellular l...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4180857/ https://www.ncbi.nlm.nih.gov/pubmed/25260869 http://dx.doi.org/10.1186/s12870-014-0252-0 |
_version_ | 1782337273128288256 |
---|---|
author | Berson, Tobias von Wangenheim, Daniel Takáč, Tomáš Šamajová, Olga Rosero, Amparo Ovečka, Miroslav Komis, George Stelzer, Ernst HK Šamaj, Jozef |
author_facet | Berson, Tobias von Wangenheim, Daniel Takáč, Tomáš Šamajová, Olga Rosero, Amparo Ovečka, Miroslav Komis, George Stelzer, Ernst HK Šamaj, Jozef |
author_sort | Berson, Tobias |
collection | PubMed |
description | BACKGROUND: Small Rab GTPases are important regulators of vesicular trafficking in plants. AtRabA1d, a member of the RabA1 subfamily of small GTPases, was previously found in the vesicle-rich apical dome of growing root hairs suggesting a role during tip growth; however, its specific intracellular localization and role in plants has not been well described. RESULTS: The transient expression of 35S::GFP:RabA1d construct in Allium porrum and Nicotiana benthamiana revealed vesicular structures, which were further corroborated in stable transformed Arabidopsis thaliana plants. GFP-RabA1d colocalized with the trans-Golgi network marker mCherry-VTI12 and with early FM4-64-labeled endosomal compartments. Late endosomes and endoplasmic reticulum labeled with FYVE-DsRed and ER-DsRed, respectively, were devoid of GFP-RabA1d. The accumulation of GFP-RabA1d in the core of brefeldin A (BFA)-induced-compartments and the quantitative upregulation of RabA1d protein levels after BFA treatment confirmed the association of RabA1d with early endosomes/TGN and its role in vesicle trafficking. Light-sheet microscopy revealed involvement of RabA1d in root development. In root cells, GFP-RabA1d followed cell plate expansion consistently with cytokinesis-related vesicular trafficking and membrane recycling. GFP-RabA1d accumulated in disc-like structures of nascent cell plates, which progressively evolved to marginal ring-like structures of the growing cell plates. During root hair growth and development, GFP-RabA1d was enriched at root hair bulges and at the apical dome of vigorously elongating root hairs. Importantly, GFP-RabA1d signal intensity exhibited an oscillatory behavior in-phase with tip growth. Progressively, this tip localization dissapeared in mature root hairs suggesting a link between tip localization of RabA1d and root hair elongation. Our results support a RabA1d role in events that require vigorous membrane trafficking. CONCLUSIONS: RabA1d is located in early endosomes/TGN and is involved in vesicle trafficking. RabA1d participates in both cell plate formation and root hair oscillatory tip growth. The specific GFP-RabA1d subcellular localization confirms a correlation between its specific spatio-temporal accumulation and local vesicle trafficking requirements during cell plate and root hair formation. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-014-0252-0) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4180857 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-41808572014-10-03 Trans-Golgi network localized small GTPase RabA1d is involved in cell plate formation and oscillatory root hair growth Berson, Tobias von Wangenheim, Daniel Takáč, Tomáš Šamajová, Olga Rosero, Amparo Ovečka, Miroslav Komis, George Stelzer, Ernst HK Šamaj, Jozef BMC Plant Biol Research Article BACKGROUND: Small Rab GTPases are important regulators of vesicular trafficking in plants. AtRabA1d, a member of the RabA1 subfamily of small GTPases, was previously found in the vesicle-rich apical dome of growing root hairs suggesting a role during tip growth; however, its specific intracellular localization and role in plants has not been well described. RESULTS: The transient expression of 35S::GFP:RabA1d construct in Allium porrum and Nicotiana benthamiana revealed vesicular structures, which were further corroborated in stable transformed Arabidopsis thaliana plants. GFP-RabA1d colocalized with the trans-Golgi network marker mCherry-VTI12 and with early FM4-64-labeled endosomal compartments. Late endosomes and endoplasmic reticulum labeled with FYVE-DsRed and ER-DsRed, respectively, were devoid of GFP-RabA1d. The accumulation of GFP-RabA1d in the core of brefeldin A (BFA)-induced-compartments and the quantitative upregulation of RabA1d protein levels after BFA treatment confirmed the association of RabA1d with early endosomes/TGN and its role in vesicle trafficking. Light-sheet microscopy revealed involvement of RabA1d in root development. In root cells, GFP-RabA1d followed cell plate expansion consistently with cytokinesis-related vesicular trafficking and membrane recycling. GFP-RabA1d accumulated in disc-like structures of nascent cell plates, which progressively evolved to marginal ring-like structures of the growing cell plates. During root hair growth and development, GFP-RabA1d was enriched at root hair bulges and at the apical dome of vigorously elongating root hairs. Importantly, GFP-RabA1d signal intensity exhibited an oscillatory behavior in-phase with tip growth. Progressively, this tip localization dissapeared in mature root hairs suggesting a link between tip localization of RabA1d and root hair elongation. Our results support a RabA1d role in events that require vigorous membrane trafficking. CONCLUSIONS: RabA1d is located in early endosomes/TGN and is involved in vesicle trafficking. RabA1d participates in both cell plate formation and root hair oscillatory tip growth. The specific GFP-RabA1d subcellular localization confirms a correlation between its specific spatio-temporal accumulation and local vesicle trafficking requirements during cell plate and root hair formation. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-014-0252-0) contains supplementary material, which is available to authorized users. BioMed Central 2014-09-27 /pmc/articles/PMC4180857/ /pubmed/25260869 http://dx.doi.org/10.1186/s12870-014-0252-0 Text en © Berson et al.; licensee BioMed Central Ltd. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Berson, Tobias von Wangenheim, Daniel Takáč, Tomáš Šamajová, Olga Rosero, Amparo Ovečka, Miroslav Komis, George Stelzer, Ernst HK Šamaj, Jozef Trans-Golgi network localized small GTPase RabA1d is involved in cell plate formation and oscillatory root hair growth |
title | Trans-Golgi network localized small GTPase RabA1d is involved in cell plate formation and oscillatory root hair growth |
title_full | Trans-Golgi network localized small GTPase RabA1d is involved in cell plate formation and oscillatory root hair growth |
title_fullStr | Trans-Golgi network localized small GTPase RabA1d is involved in cell plate formation and oscillatory root hair growth |
title_full_unstemmed | Trans-Golgi network localized small GTPase RabA1d is involved in cell plate formation and oscillatory root hair growth |
title_short | Trans-Golgi network localized small GTPase RabA1d is involved in cell plate formation and oscillatory root hair growth |
title_sort | trans-golgi network localized small gtpase raba1d is involved in cell plate formation and oscillatory root hair growth |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4180857/ https://www.ncbi.nlm.nih.gov/pubmed/25260869 http://dx.doi.org/10.1186/s12870-014-0252-0 |
work_keys_str_mv | AT bersontobias transgolginetworklocalizedsmallgtpaseraba1disinvolvedincellplateformationandoscillatoryroothairgrowth AT vonwangenheimdaniel transgolginetworklocalizedsmallgtpaseraba1disinvolvedincellplateformationandoscillatoryroothairgrowth AT takactomas transgolginetworklocalizedsmallgtpaseraba1disinvolvedincellplateformationandoscillatoryroothairgrowth AT samajovaolga transgolginetworklocalizedsmallgtpaseraba1disinvolvedincellplateformationandoscillatoryroothairgrowth AT roseroamparo transgolginetworklocalizedsmallgtpaseraba1disinvolvedincellplateformationandoscillatoryroothairgrowth AT oveckamiroslav transgolginetworklocalizedsmallgtpaseraba1disinvolvedincellplateformationandoscillatoryroothairgrowth AT komisgeorge transgolginetworklocalizedsmallgtpaseraba1disinvolvedincellplateformationandoscillatoryroothairgrowth AT stelzerernsthk transgolginetworklocalizedsmallgtpaseraba1disinvolvedincellplateformationandoscillatoryroothairgrowth AT samajjozef transgolginetworklocalizedsmallgtpaseraba1disinvolvedincellplateformationandoscillatoryroothairgrowth |