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Trans-Golgi network localized small GTPase RabA1d is involved in cell plate formation and oscillatory root hair growth

BACKGROUND: Small Rab GTPases are important regulators of vesicular trafficking in plants. AtRabA1d, a member of the RabA1 subfamily of small GTPases, was previously found in the vesicle-rich apical dome of growing root hairs suggesting a role during tip growth; however, its specific intracellular l...

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Autores principales: Berson, Tobias, von Wangenheim, Daniel, Takáč, Tomáš, Šamajová, Olga, Rosero, Amparo, Ovečka, Miroslav, Komis, George, Stelzer, Ernst HK, Šamaj, Jozef
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4180857/
https://www.ncbi.nlm.nih.gov/pubmed/25260869
http://dx.doi.org/10.1186/s12870-014-0252-0
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author Berson, Tobias
von Wangenheim, Daniel
Takáč, Tomáš
Šamajová, Olga
Rosero, Amparo
Ovečka, Miroslav
Komis, George
Stelzer, Ernst HK
Šamaj, Jozef
author_facet Berson, Tobias
von Wangenheim, Daniel
Takáč, Tomáš
Šamajová, Olga
Rosero, Amparo
Ovečka, Miroslav
Komis, George
Stelzer, Ernst HK
Šamaj, Jozef
author_sort Berson, Tobias
collection PubMed
description BACKGROUND: Small Rab GTPases are important regulators of vesicular trafficking in plants. AtRabA1d, a member of the RabA1 subfamily of small GTPases, was previously found in the vesicle-rich apical dome of growing root hairs suggesting a role during tip growth; however, its specific intracellular localization and role in plants has not been well described. RESULTS: The transient expression of 35S::GFP:RabA1d construct in Allium porrum and Nicotiana benthamiana revealed vesicular structures, which were further corroborated in stable transformed Arabidopsis thaliana plants. GFP-RabA1d colocalized with the trans-Golgi network marker mCherry-VTI12 and with early FM4-64-labeled endosomal compartments. Late endosomes and endoplasmic reticulum labeled with FYVE-DsRed and ER-DsRed, respectively, were devoid of GFP-RabA1d. The accumulation of GFP-RabA1d in the core of brefeldin A (BFA)-induced-compartments and the quantitative upregulation of RabA1d protein levels after BFA treatment confirmed the association of RabA1d with early endosomes/TGN and its role in vesicle trafficking. Light-sheet microscopy revealed involvement of RabA1d in root development. In root cells, GFP-RabA1d followed cell plate expansion consistently with cytokinesis-related vesicular trafficking and membrane recycling. GFP-RabA1d accumulated in disc-like structures of nascent cell plates, which progressively evolved to marginal ring-like structures of the growing cell plates. During root hair growth and development, GFP-RabA1d was enriched at root hair bulges and at the apical dome of vigorously elongating root hairs. Importantly, GFP-RabA1d signal intensity exhibited an oscillatory behavior in-phase with tip growth. Progressively, this tip localization dissapeared in mature root hairs suggesting a link between tip localization of RabA1d and root hair elongation. Our results support a RabA1d role in events that require vigorous membrane trafficking. CONCLUSIONS: RabA1d is located in early endosomes/TGN and is involved in vesicle trafficking. RabA1d participates in both cell plate formation and root hair oscillatory tip growth. The specific GFP-RabA1d subcellular localization confirms a correlation between its specific spatio-temporal accumulation and local vesicle trafficking requirements during cell plate and root hair formation. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-014-0252-0) contains supplementary material, which is available to authorized users.
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spelling pubmed-41808572014-10-03 Trans-Golgi network localized small GTPase RabA1d is involved in cell plate formation and oscillatory root hair growth Berson, Tobias von Wangenheim, Daniel Takáč, Tomáš Šamajová, Olga Rosero, Amparo Ovečka, Miroslav Komis, George Stelzer, Ernst HK Šamaj, Jozef BMC Plant Biol Research Article BACKGROUND: Small Rab GTPases are important regulators of vesicular trafficking in plants. AtRabA1d, a member of the RabA1 subfamily of small GTPases, was previously found in the vesicle-rich apical dome of growing root hairs suggesting a role during tip growth; however, its specific intracellular localization and role in plants has not been well described. RESULTS: The transient expression of 35S::GFP:RabA1d construct in Allium porrum and Nicotiana benthamiana revealed vesicular structures, which were further corroborated in stable transformed Arabidopsis thaliana plants. GFP-RabA1d colocalized with the trans-Golgi network marker mCherry-VTI12 and with early FM4-64-labeled endosomal compartments. Late endosomes and endoplasmic reticulum labeled with FYVE-DsRed and ER-DsRed, respectively, were devoid of GFP-RabA1d. The accumulation of GFP-RabA1d in the core of brefeldin A (BFA)-induced-compartments and the quantitative upregulation of RabA1d protein levels after BFA treatment confirmed the association of RabA1d with early endosomes/TGN and its role in vesicle trafficking. Light-sheet microscopy revealed involvement of RabA1d in root development. In root cells, GFP-RabA1d followed cell plate expansion consistently with cytokinesis-related vesicular trafficking and membrane recycling. GFP-RabA1d accumulated in disc-like structures of nascent cell plates, which progressively evolved to marginal ring-like structures of the growing cell plates. During root hair growth and development, GFP-RabA1d was enriched at root hair bulges and at the apical dome of vigorously elongating root hairs. Importantly, GFP-RabA1d signal intensity exhibited an oscillatory behavior in-phase with tip growth. Progressively, this tip localization dissapeared in mature root hairs suggesting a link between tip localization of RabA1d and root hair elongation. Our results support a RabA1d role in events that require vigorous membrane trafficking. CONCLUSIONS: RabA1d is located in early endosomes/TGN and is involved in vesicle trafficking. RabA1d participates in both cell plate formation and root hair oscillatory tip growth. The specific GFP-RabA1d subcellular localization confirms a correlation between its specific spatio-temporal accumulation and local vesicle trafficking requirements during cell plate and root hair formation. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-014-0252-0) contains supplementary material, which is available to authorized users. BioMed Central 2014-09-27 /pmc/articles/PMC4180857/ /pubmed/25260869 http://dx.doi.org/10.1186/s12870-014-0252-0 Text en © Berson et al.; licensee BioMed Central Ltd. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Berson, Tobias
von Wangenheim, Daniel
Takáč, Tomáš
Šamajová, Olga
Rosero, Amparo
Ovečka, Miroslav
Komis, George
Stelzer, Ernst HK
Šamaj, Jozef
Trans-Golgi network localized small GTPase RabA1d is involved in cell plate formation and oscillatory root hair growth
title Trans-Golgi network localized small GTPase RabA1d is involved in cell plate formation and oscillatory root hair growth
title_full Trans-Golgi network localized small GTPase RabA1d is involved in cell plate formation and oscillatory root hair growth
title_fullStr Trans-Golgi network localized small GTPase RabA1d is involved in cell plate formation and oscillatory root hair growth
title_full_unstemmed Trans-Golgi network localized small GTPase RabA1d is involved in cell plate formation and oscillatory root hair growth
title_short Trans-Golgi network localized small GTPase RabA1d is involved in cell plate formation and oscillatory root hair growth
title_sort trans-golgi network localized small gtpase raba1d is involved in cell plate formation and oscillatory root hair growth
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4180857/
https://www.ncbi.nlm.nih.gov/pubmed/25260869
http://dx.doi.org/10.1186/s12870-014-0252-0
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