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Biological Control of Meloidogyne hapla Using an Antagonistic Bacterium
We examined the efficacy of a bacterium for biocontrol of the root-knot nematode (RKN) Meloidogyne hapla in carrot (Daucus carota subsp. sativus) and tomato (Solanum lycopersicum). Among 542 bacterial isolates from various soils and plants, the highest nematode mortality was observed for treatments...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Korean Society of Plant Pathology
2014
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4181115/ https://www.ncbi.nlm.nih.gov/pubmed/25289015 http://dx.doi.org/10.5423/PPJ.OA.02.2014.0013 |
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author | Park, Jiyeong Seo, Yunhee Kim, Young Ho |
author_facet | Park, Jiyeong Seo, Yunhee Kim, Young Ho |
author_sort | Park, Jiyeong |
collection | PubMed |
description | We examined the efficacy of a bacterium for biocontrol of the root-knot nematode (RKN) Meloidogyne hapla in carrot (Daucus carota subsp. sativus) and tomato (Solanum lycopersicum). Among 542 bacterial isolates from various soils and plants, the highest nematode mortality was observed for treatments with isolate C1-7, which was identified as Bacillus cereus based on cultural and morphological characteristics, the Biolog program, and 16S rRNA sequencing analyses. The population density and the nematicidal activity of B. cereus C1-7 remained high until the end of culture in brain heart infusion broth, suggesting that it may have sustainable biocontrol potential. In pot experiments, the biocontrol efficacy of B. cereus C1-7 was high, showing complete inhibition of root gall or egg mass formation by RKN in carrot and tomato plants, and subsequently reducing RKN damage and suppressing nematode population growth, respectively. Light microscopy of RKN-infected carrot root tissues treated with C1-7 showed reduced formation of gall cells and fully developed giant cells, while extensive gall cells and fully mature giant cells with prominent cell wall ingrowths formed in the untreated control plants infected with RKNs. These histopathological characteristics may be the result of residual or systemic biocontrol activity of the bacterium, which may coincide with the biocontrol efficacies of nematodes in pots. These results suggest that B. cereus C1-7 can be used as a biocontrol agent for M. hapla. |
format | Online Article Text |
id | pubmed-4181115 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Korean Society of Plant Pathology |
record_format | MEDLINE/PubMed |
spelling | pubmed-41811152014-10-06 Biological Control of Meloidogyne hapla Using an Antagonistic Bacterium Park, Jiyeong Seo, Yunhee Kim, Young Ho Plant Pathol J Articles We examined the efficacy of a bacterium for biocontrol of the root-knot nematode (RKN) Meloidogyne hapla in carrot (Daucus carota subsp. sativus) and tomato (Solanum lycopersicum). Among 542 bacterial isolates from various soils and plants, the highest nematode mortality was observed for treatments with isolate C1-7, which was identified as Bacillus cereus based on cultural and morphological characteristics, the Biolog program, and 16S rRNA sequencing analyses. The population density and the nematicidal activity of B. cereus C1-7 remained high until the end of culture in brain heart infusion broth, suggesting that it may have sustainable biocontrol potential. In pot experiments, the biocontrol efficacy of B. cereus C1-7 was high, showing complete inhibition of root gall or egg mass formation by RKN in carrot and tomato plants, and subsequently reducing RKN damage and suppressing nematode population growth, respectively. Light microscopy of RKN-infected carrot root tissues treated with C1-7 showed reduced formation of gall cells and fully developed giant cells, while extensive gall cells and fully mature giant cells with prominent cell wall ingrowths formed in the untreated control plants infected with RKNs. These histopathological characteristics may be the result of residual or systemic biocontrol activity of the bacterium, which may coincide with the biocontrol efficacies of nematodes in pots. These results suggest that B. cereus C1-7 can be used as a biocontrol agent for M. hapla. Korean Society of Plant Pathology 2014-09 /pmc/articles/PMC4181115/ /pubmed/25289015 http://dx.doi.org/10.5423/PPJ.OA.02.2014.0013 Text en © The Korean Society of Plant Pathology This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Park, Jiyeong Seo, Yunhee Kim, Young Ho Biological Control of Meloidogyne hapla Using an Antagonistic Bacterium |
title | Biological Control of Meloidogyne hapla Using an Antagonistic Bacterium |
title_full | Biological Control of Meloidogyne hapla Using an Antagonistic Bacterium |
title_fullStr | Biological Control of Meloidogyne hapla Using an Antagonistic Bacterium |
title_full_unstemmed | Biological Control of Meloidogyne hapla Using an Antagonistic Bacterium |
title_short | Biological Control of Meloidogyne hapla Using an Antagonistic Bacterium |
title_sort | biological control of meloidogyne hapla using an antagonistic bacterium |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4181115/ https://www.ncbi.nlm.nih.gov/pubmed/25289015 http://dx.doi.org/10.5423/PPJ.OA.02.2014.0013 |
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