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A Rapid and Efficient Method for Construction of an Infectious Clone of Tomato yellow leaf curl virus
Tomato yellow leaf curl virus (TYLCV), a member of the genus Begomovirus, is responsible for one of the most devastating viral diseases in tomato-growing countries and is becoming a serious problem in many subtropical and tropical countries. The climate in Korea is getting warmer and developing subt...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Korean Society of Plant Pathology
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4181116/ https://www.ncbi.nlm.nih.gov/pubmed/25289018 http://dx.doi.org/10.5423/PPJ.NT.03.2014.0025 |
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author | Bang, Bongjun Lee, Jongyun Kim, Sunyoung Park, Jungwook Nguyen, Thao Thi Seo, Young-Su |
author_facet | Bang, Bongjun Lee, Jongyun Kim, Sunyoung Park, Jungwook Nguyen, Thao Thi Seo, Young-Su |
author_sort | Bang, Bongjun |
collection | PubMed |
description | Tomato yellow leaf curl virus (TYLCV), a member of the genus Begomovirus, is responsible for one of the most devastating viral diseases in tomato-growing countries and is becoming a serious problem in many subtropical and tropical countries. The climate in Korea is getting warmer and developing subtropical features in response to global warming. These changes are being accompanied by TYLCV, which is now becoming a large problem in the Korean tomato industry. The most effective way to reduce damage caused by TYLCV is to breed resistant varieties of tomatoes. To accomplish this, it is necessary to establish a simple inoculation technique for the efficient evaluation of resistance to TYLCV. Here, we present the rolling circle amplification (RCA) method, which employs a bacteriophage using phi-29 DNA polymerase for construction of infectious TYLCV clones. The RCA method is simple, does not require sequence information for cloning, and is less expensive and time consuming than conventional PCR based-methods. Furthermore, RCA-based construction of an infectious clone can be very useful to other emerging and unknown geminiviruses in Korea. |
format | Online Article Text |
id | pubmed-4181116 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Korean Society of Plant Pathology |
record_format | MEDLINE/PubMed |
spelling | pubmed-41811162014-10-06 A Rapid and Efficient Method for Construction of an Infectious Clone of Tomato yellow leaf curl virus Bang, Bongjun Lee, Jongyun Kim, Sunyoung Park, Jungwook Nguyen, Thao Thi Seo, Young-Su Plant Pathol J Note Tomato yellow leaf curl virus (TYLCV), a member of the genus Begomovirus, is responsible for one of the most devastating viral diseases in tomato-growing countries and is becoming a serious problem in many subtropical and tropical countries. The climate in Korea is getting warmer and developing subtropical features in response to global warming. These changes are being accompanied by TYLCV, which is now becoming a large problem in the Korean tomato industry. The most effective way to reduce damage caused by TYLCV is to breed resistant varieties of tomatoes. To accomplish this, it is necessary to establish a simple inoculation technique for the efficient evaluation of resistance to TYLCV. Here, we present the rolling circle amplification (RCA) method, which employs a bacteriophage using phi-29 DNA polymerase for construction of infectious TYLCV clones. The RCA method is simple, does not require sequence information for cloning, and is less expensive and time consuming than conventional PCR based-methods. Furthermore, RCA-based construction of an infectious clone can be very useful to other emerging and unknown geminiviruses in Korea. Korean Society of Plant Pathology 2014-09 /pmc/articles/PMC4181116/ /pubmed/25289018 http://dx.doi.org/10.5423/PPJ.NT.03.2014.0025 Text en © The Korean Society of Plant Pathology This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Note Bang, Bongjun Lee, Jongyun Kim, Sunyoung Park, Jungwook Nguyen, Thao Thi Seo, Young-Su A Rapid and Efficient Method for Construction of an Infectious Clone of Tomato yellow leaf curl virus |
title | A Rapid and Efficient Method for Construction of an Infectious Clone of Tomato yellow leaf curl virus |
title_full | A Rapid and Efficient Method for Construction of an Infectious Clone of Tomato yellow leaf curl virus |
title_fullStr | A Rapid and Efficient Method for Construction of an Infectious Clone of Tomato yellow leaf curl virus |
title_full_unstemmed | A Rapid and Efficient Method for Construction of an Infectious Clone of Tomato yellow leaf curl virus |
title_short | A Rapid and Efficient Method for Construction of an Infectious Clone of Tomato yellow leaf curl virus |
title_sort | rapid and efficient method for construction of an infectious clone of tomato yellow leaf curl virus |
topic | Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4181116/ https://www.ncbi.nlm.nih.gov/pubmed/25289018 http://dx.doi.org/10.5423/PPJ.NT.03.2014.0025 |
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