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Inhibition of biofilm formation and lipase in Candida albicans by culture filtrate of Staphylococcus epidermidis in vitro

BACKGROUND: Candida spp. are fourth most common cause of bloodstream infection in developed countries and emerging agents of fungemia in developing countries, with considerable attributable mortality. Candidemia is associated with the formation of complex, structured microbial communities called bio...

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Autores principales: Bhattacharyya, Sayan, Gupta, Prashant, Banerjee, Gopa, Jain, Amita, Singh, Mastan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4181128/
https://www.ncbi.nlm.nih.gov/pubmed/25298939
http://dx.doi.org/10.4103/2229-516X.140721
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author Bhattacharyya, Sayan
Gupta, Prashant
Banerjee, Gopa
Jain, Amita
Singh, Mastan
author_facet Bhattacharyya, Sayan
Gupta, Prashant
Banerjee, Gopa
Jain, Amita
Singh, Mastan
author_sort Bhattacharyya, Sayan
collection PubMed
description BACKGROUND: Candida spp. are fourth most common cause of bloodstream infection in developed countries and emerging agents of fungemia in developing countries, with considerable attributable mortality. Candidemia is associated with the formation of complex, structured microbial communities called biofilms. Biofilm formation makes treatment difficult due to improper drug penetration and factors like high cost and adverse effects of antifungal drugs available. Hence, low-cost alternatives are urgently required to treat device-associated invasive candidiasis. OBJECTIVES: To study the effect of culture filtrate of Staphylococcus epidermidis on biofilm formation and lipase expression of Candida albicans in vitro. MATERIALS AND METHODS: Yeast cells isolated from clinical samples were suspended to a turbidity of 10(6) in (a) Yeast extract-peptone-dextrose (YPD) broth and (b) culture filtrate, and 100 μl of each were dispensed in separate wells of microtiter plate. After repeated washing and reloading with respective liquid media, readings were taken spectrophotometrically. To check for lipase inhibition, yeasts were incubated overnight in YPD and filtrate and subcultured on media containing Tween-80 and CaCl(2). Positive lipase activity was denoted by haziness around colonies. RESULTS: Mean reading of C. albicans in YPD broth was 0.579 while the same when yeasts were suspended in S. epidermidis culture filtrate was 0.281 (P < 0.05 by Z-test of significance). Lipase of C. albicans was inhibited by culture filtrate. Filtrate was found to be nontoxic to human cell line. CONCLUSIONS: Culture filtrate of S. epidermidis can hence pave the way for development of new strategies to inhibit biofilm formation in device-associated candidemia.
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spelling pubmed-41811282014-10-08 Inhibition of biofilm formation and lipase in Candida albicans by culture filtrate of Staphylococcus epidermidis in vitro Bhattacharyya, Sayan Gupta, Prashant Banerjee, Gopa Jain, Amita Singh, Mastan Int J Appl Basic Med Res Original Article BACKGROUND: Candida spp. are fourth most common cause of bloodstream infection in developed countries and emerging agents of fungemia in developing countries, with considerable attributable mortality. Candidemia is associated with the formation of complex, structured microbial communities called biofilms. Biofilm formation makes treatment difficult due to improper drug penetration and factors like high cost and adverse effects of antifungal drugs available. Hence, low-cost alternatives are urgently required to treat device-associated invasive candidiasis. OBJECTIVES: To study the effect of culture filtrate of Staphylococcus epidermidis on biofilm formation and lipase expression of Candida albicans in vitro. MATERIALS AND METHODS: Yeast cells isolated from clinical samples were suspended to a turbidity of 10(6) in (a) Yeast extract-peptone-dextrose (YPD) broth and (b) culture filtrate, and 100 μl of each were dispensed in separate wells of microtiter plate. After repeated washing and reloading with respective liquid media, readings were taken spectrophotometrically. To check for lipase inhibition, yeasts were incubated overnight in YPD and filtrate and subcultured on media containing Tween-80 and CaCl(2). Positive lipase activity was denoted by haziness around colonies. RESULTS: Mean reading of C. albicans in YPD broth was 0.579 while the same when yeasts were suspended in S. epidermidis culture filtrate was 0.281 (P < 0.05 by Z-test of significance). Lipase of C. albicans was inhibited by culture filtrate. Filtrate was found to be nontoxic to human cell line. CONCLUSIONS: Culture filtrate of S. epidermidis can hence pave the way for development of new strategies to inhibit biofilm formation in device-associated candidemia. Medknow Publications & Media Pvt Ltd 2014-09 /pmc/articles/PMC4181128/ /pubmed/25298939 http://dx.doi.org/10.4103/2229-516X.140721 Text en Copyright: © International Journal of Applied and Basic Medical Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Bhattacharyya, Sayan
Gupta, Prashant
Banerjee, Gopa
Jain, Amita
Singh, Mastan
Inhibition of biofilm formation and lipase in Candida albicans by culture filtrate of Staphylococcus epidermidis in vitro
title Inhibition of biofilm formation and lipase in Candida albicans by culture filtrate of Staphylococcus epidermidis in vitro
title_full Inhibition of biofilm formation and lipase in Candida albicans by culture filtrate of Staphylococcus epidermidis in vitro
title_fullStr Inhibition of biofilm formation and lipase in Candida albicans by culture filtrate of Staphylococcus epidermidis in vitro
title_full_unstemmed Inhibition of biofilm formation and lipase in Candida albicans by culture filtrate of Staphylococcus epidermidis in vitro
title_short Inhibition of biofilm formation and lipase in Candida albicans by culture filtrate of Staphylococcus epidermidis in vitro
title_sort inhibition of biofilm formation and lipase in candida albicans by culture filtrate of staphylococcus epidermidis in vitro
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4181128/
https://www.ncbi.nlm.nih.gov/pubmed/25298939
http://dx.doi.org/10.4103/2229-516X.140721
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