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Sirtuin 1 Activity in Peripheral Blood Mononuclear Cells of Patients with Osteoporosis
BACKGROUND: Sirtuin 1 (SIRT1) is a class III histone deacetylase that may play a critical role in several biological functions, including lifespan, stress, and inflammation. Our main objective was to evaluate SIRT1 activity in peripheral blood mononuclear cells (PBMCs) in patients with osteoporosis...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4181306/ https://www.ncbi.nlm.nih.gov/pubmed/25228047 http://dx.doi.org/10.12659/MSMBR.891372 |
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author | Godfrin-Valnet, Marie Khan, Kashif A. Guillot, Xavier Prati, Clément Baud, Lucile Abbas, Wasim Toussirot, Eric Wendling, Daniel Herbein, Georges |
author_facet | Godfrin-Valnet, Marie Khan, Kashif A. Guillot, Xavier Prati, Clément Baud, Lucile Abbas, Wasim Toussirot, Eric Wendling, Daniel Herbein, Georges |
author_sort | Godfrin-Valnet, Marie |
collection | PubMed |
description | BACKGROUND: Sirtuin 1 (SIRT1) is a class III histone deacetylase that may play a critical role in several biological functions, including lifespan, stress, and inflammation. Our main objective was to evaluate SIRT1 activity in peripheral blood mononuclear cells (PBMCs) in patients with osteoporosis and to analyze the relationship between the SIRT 1 activity and markers of inflammation and bone remodelling. MATERIAL/METHODS: We performed a prospective monocentric study of patients with osteoporosis and measured the nuclear and cytoplasmic activities of SIRT1 in PBMCs. Levels of proinflammatory cytokines were assessed in culture supernatants of PBMCs isolated from the osteoporosis patients. The level of serum C-terminal cross-linking telopeptide of type I collagen (CTX), a marker of bone resorption, was measured in the serum of osteoporosis patients. RESULTS: Sixteen women with osteoporosis were included. A statistically significant correlation between the cytoplasmic and nuclear SIRT 1 activities was found in PBMCs of patients with osteoporosis. Although non-significant, we observed a negative trend between nuclear SIRT 1 activity and the rate of serum CTX and a positive trend between IL-6 and CTX levels in patients with osteoporosis. CONCLUSIONS: This study shows that the cytoplasmic and nuclear SIRT 1 activities are measurable in circulating PBMCs of patients with osteoporosis and that these 2 activities are correlated. The potential role of inflammation in bone resorption in patients with osteoporosis was also studied. |
format | Online Article Text |
id | pubmed-4181306 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-41813062014-10-02 Sirtuin 1 Activity in Peripheral Blood Mononuclear Cells of Patients with Osteoporosis Godfrin-Valnet, Marie Khan, Kashif A. Guillot, Xavier Prati, Clément Baud, Lucile Abbas, Wasim Toussirot, Eric Wendling, Daniel Herbein, Georges Med Sci Monit Basic Res Molecular Biology BACKGROUND: Sirtuin 1 (SIRT1) is a class III histone deacetylase that may play a critical role in several biological functions, including lifespan, stress, and inflammation. Our main objective was to evaluate SIRT1 activity in peripheral blood mononuclear cells (PBMCs) in patients with osteoporosis and to analyze the relationship between the SIRT 1 activity and markers of inflammation and bone remodelling. MATERIAL/METHODS: We performed a prospective monocentric study of patients with osteoporosis and measured the nuclear and cytoplasmic activities of SIRT1 in PBMCs. Levels of proinflammatory cytokines were assessed in culture supernatants of PBMCs isolated from the osteoporosis patients. The level of serum C-terminal cross-linking telopeptide of type I collagen (CTX), a marker of bone resorption, was measured in the serum of osteoporosis patients. RESULTS: Sixteen women with osteoporosis were included. A statistically significant correlation between the cytoplasmic and nuclear SIRT 1 activities was found in PBMCs of patients with osteoporosis. Although non-significant, we observed a negative trend between nuclear SIRT 1 activity and the rate of serum CTX and a positive trend between IL-6 and CTX levels in patients with osteoporosis. CONCLUSIONS: This study shows that the cytoplasmic and nuclear SIRT 1 activities are measurable in circulating PBMCs of patients with osteoporosis and that these 2 activities are correlated. The potential role of inflammation in bone resorption in patients with osteoporosis was also studied. International Scientific Literature, Inc. 2014-09-17 /pmc/articles/PMC4181306/ /pubmed/25228047 http://dx.doi.org/10.12659/MSMBR.891372 Text en © Med Sci Monit, 2014 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License |
spellingShingle | Molecular Biology Godfrin-Valnet, Marie Khan, Kashif A. Guillot, Xavier Prati, Clément Baud, Lucile Abbas, Wasim Toussirot, Eric Wendling, Daniel Herbein, Georges Sirtuin 1 Activity in Peripheral Blood Mononuclear Cells of Patients with Osteoporosis |
title | Sirtuin 1 Activity in Peripheral Blood Mononuclear Cells of Patients with Osteoporosis |
title_full | Sirtuin 1 Activity in Peripheral Blood Mononuclear Cells of Patients with Osteoporosis |
title_fullStr | Sirtuin 1 Activity in Peripheral Blood Mononuclear Cells of Patients with Osteoporosis |
title_full_unstemmed | Sirtuin 1 Activity in Peripheral Blood Mononuclear Cells of Patients with Osteoporosis |
title_short | Sirtuin 1 Activity in Peripheral Blood Mononuclear Cells of Patients with Osteoporosis |
title_sort | sirtuin 1 activity in peripheral blood mononuclear cells of patients with osteoporosis |
topic | Molecular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4181306/ https://www.ncbi.nlm.nih.gov/pubmed/25228047 http://dx.doi.org/10.12659/MSMBR.891372 |
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