Probing the Crucial Role of Leu31 and Thr33 of the Bacillus pumilus CBS Alkaline Protease in Substrate Recognition and Enzymatic Depilation of Animal Hide

The sapB gene, encoding Bacillus pumilus CBS protease, and seven mutated genes (sapB-L31I, sapB-T33S, sapB-N99Y, sapB-L31I/T33S, sapB-L31I/N99Y, sapB-T33S/N99Y, and sapB-L31I/T33S/N99Y) were overexpressed in protease-deficient Bacillus subtilis DB430 and purified to homogeneity. SAPB-N99Y and rSAPB...

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Detalles Bibliográficos
Autores principales: Zaraî Jaouadi, Nadia, Jaouadi, Bassem, Ben Hlima, Hajer, Rekik, Hatem, Belhoul, Mouna, Hmidi, Maher, Aicha, Houda Slimene Ben, Hila, Chiraz Gorgi, Toumi, Abdessatar, Aghajari, Nushin, Bejar, Samir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4181652/
https://www.ncbi.nlm.nih.gov/pubmed/25264614
http://dx.doi.org/10.1371/journal.pone.0108367

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