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Differentially Expressed Genes of Virulent and Nonvirulent Entamoeba histolytica Strains Identified by Suppression Subtractive Hybridization

Entamoeba histolytica is a parasite which presents capacity to degrade tissues and therefore has a pathogenic behavior. As this behavior is not shown by all strains, there have been several studies investigating molecular basis of the cytotoxicity process. Using the suppression subtractive hybridiza...

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Autores principales: Freitas, Michelle A. R., Alvarenga, Ângela C., Fernandes, Helen C., Gil, Frederico F., Melo, Maria N., Pesquero, Jorge L., Gomes, Maria A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4182305/
https://www.ncbi.nlm.nih.gov/pubmed/25313356
http://dx.doi.org/10.1155/2014/285607
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author Freitas, Michelle A. R.
Alvarenga, Ângela C.
Fernandes, Helen C.
Gil, Frederico F.
Melo, Maria N.
Pesquero, Jorge L.
Gomes, Maria A.
author_facet Freitas, Michelle A. R.
Alvarenga, Ângela C.
Fernandes, Helen C.
Gil, Frederico F.
Melo, Maria N.
Pesquero, Jorge L.
Gomes, Maria A.
author_sort Freitas, Michelle A. R.
collection PubMed
description Entamoeba histolytica is a parasite which presents capacity to degrade tissues and therefore has a pathogenic behavior. As this behavior is not shown by all strains, there have been several studies investigating molecular basis of the cytotoxicity process. Using the suppression subtractive hybridization (SSH) technique, differential gene expressions of two E. histolytica strains, one virulent (EGG) and one nonvirulent (452), have been analyzed with the purpose of isolating genes which may be involved with amoebic virulence. Nine cDNA fragments presenting high homology with E. histolytica previously sequenced genes were subtracted. Of these, four genes were confirmed by RT-PCR. Two coding for hypothetical proteins, one for a cysteine-rich protein, expressed only in the virulent strain, EGG and another one, coding for grainin 2 protein, exclusive from 452 strain. This study provided new insight into the proteins differences in the virulent and nonvirulent E. histolytica strains. We believe that further studies with these proteins may prove association of them with tissue damage, providing new perceptions to improve treatment or diagnosis of the invasive disease.
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spelling pubmed-41823052014-10-13 Differentially Expressed Genes of Virulent and Nonvirulent Entamoeba histolytica Strains Identified by Suppression Subtractive Hybridization Freitas, Michelle A. R. Alvarenga, Ângela C. Fernandes, Helen C. Gil, Frederico F. Melo, Maria N. Pesquero, Jorge L. Gomes, Maria A. Biomed Res Int Research Article Entamoeba histolytica is a parasite which presents capacity to degrade tissues and therefore has a pathogenic behavior. As this behavior is not shown by all strains, there have been several studies investigating molecular basis of the cytotoxicity process. Using the suppression subtractive hybridization (SSH) technique, differential gene expressions of two E. histolytica strains, one virulent (EGG) and one nonvirulent (452), have been analyzed with the purpose of isolating genes which may be involved with amoebic virulence. Nine cDNA fragments presenting high homology with E. histolytica previously sequenced genes were subtracted. Of these, four genes were confirmed by RT-PCR. Two coding for hypothetical proteins, one for a cysteine-rich protein, expressed only in the virulent strain, EGG and another one, coding for grainin 2 protein, exclusive from 452 strain. This study provided new insight into the proteins differences in the virulent and nonvirulent E. histolytica strains. We believe that further studies with these proteins may prove association of them with tissue damage, providing new perceptions to improve treatment or diagnosis of the invasive disease. Hindawi Publishing Corporation 2014 2014-09-17 /pmc/articles/PMC4182305/ /pubmed/25313356 http://dx.doi.org/10.1155/2014/285607 Text en Copyright © 2014 Michelle A. R. Freitas et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Freitas, Michelle A. R.
Alvarenga, Ângela C.
Fernandes, Helen C.
Gil, Frederico F.
Melo, Maria N.
Pesquero, Jorge L.
Gomes, Maria A.
Differentially Expressed Genes of Virulent and Nonvirulent Entamoeba histolytica Strains Identified by Suppression Subtractive Hybridization
title Differentially Expressed Genes of Virulent and Nonvirulent Entamoeba histolytica Strains Identified by Suppression Subtractive Hybridization
title_full Differentially Expressed Genes of Virulent and Nonvirulent Entamoeba histolytica Strains Identified by Suppression Subtractive Hybridization
title_fullStr Differentially Expressed Genes of Virulent and Nonvirulent Entamoeba histolytica Strains Identified by Suppression Subtractive Hybridization
title_full_unstemmed Differentially Expressed Genes of Virulent and Nonvirulent Entamoeba histolytica Strains Identified by Suppression Subtractive Hybridization
title_short Differentially Expressed Genes of Virulent and Nonvirulent Entamoeba histolytica Strains Identified by Suppression Subtractive Hybridization
title_sort differentially expressed genes of virulent and nonvirulent entamoeba histolytica strains identified by suppression subtractive hybridization
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4182305/
https://www.ncbi.nlm.nih.gov/pubmed/25313356
http://dx.doi.org/10.1155/2014/285607
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