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The Mycotoxin Deoxynivalenol Predisposes for the Development of Clostridium perfringens-Induced Necrotic Enteritis in Broiler Chickens
Both mycotoxin contamination of feed and Clostridium perfringens-induced necrotic enteritis have an increasing global economic impact on poultry production. Especially the Fusarium mycotoxin deoxynivalenol (DON) is a common feed contaminant. This study aimed at examining the predisposing effect of D...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4182565/ https://www.ncbi.nlm.nih.gov/pubmed/25268498 http://dx.doi.org/10.1371/journal.pone.0108775 |
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author | Antonissen, Gunther Van Immerseel, Filip Pasmans, Frank Ducatelle, Richard Haesebrouck, Freddy Timbermont, Leen Verlinden, Marc Janssens, Geert Paul Jules Eeckhaut, Venessa Eeckhout, Mia De Saeger, Sarah Hessenberger, Sabine Martel, An Croubels, Siska |
author_facet | Antonissen, Gunther Van Immerseel, Filip Pasmans, Frank Ducatelle, Richard Haesebrouck, Freddy Timbermont, Leen Verlinden, Marc Janssens, Geert Paul Jules Eeckhaut, Venessa Eeckhout, Mia De Saeger, Sarah Hessenberger, Sabine Martel, An Croubels, Siska |
author_sort | Antonissen, Gunther |
collection | PubMed |
description | Both mycotoxin contamination of feed and Clostridium perfringens-induced necrotic enteritis have an increasing global economic impact on poultry production. Especially the Fusarium mycotoxin deoxynivalenol (DON) is a common feed contaminant. This study aimed at examining the predisposing effect of DON on the development of necrotic enteritis in broiler chickens. An experimental Clostridium perfringens infection study revealed that DON, at a contamination level of 3,000 to 4,000 µg/kg feed, increased the percentage of birds with subclinical necrotic enteritis from 20±2.6% to 47±3.0% (P<0.001). DON significantly reduced the transepithelial electrical resistance in duodenal segments (P<0.001) and decreased duodenal villus height (P = 0.014) indicating intestinal barrier disruption and intestinal epithelial damage, respectively. This may lead to an increased permeability of the intestinal epithelium and decreased absorption of dietary proteins. Protein analysis of duodenal content indeed showed that DON contamination resulted in a significant increase in total protein concentration (P = 0.023). Furthermore, DON had no effect on in vitro growth, alpha toxin production and netB toxin transcription of Clostridium perfringens. In conclusion, feed contamination with DON at concentrations below the European maximum guidance level of 5,000 µg/kg feed, is a predisposing factor for the development of necrotic enteritis in broilers. These results are associated with a negative effect of DON on the intestinal barrier function and increased intestinal protein availability, which may stimulate growth and toxin production of Clostridium perfringens. |
format | Online Article Text |
id | pubmed-4182565 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41825652014-10-07 The Mycotoxin Deoxynivalenol Predisposes for the Development of Clostridium perfringens-Induced Necrotic Enteritis in Broiler Chickens Antonissen, Gunther Van Immerseel, Filip Pasmans, Frank Ducatelle, Richard Haesebrouck, Freddy Timbermont, Leen Verlinden, Marc Janssens, Geert Paul Jules Eeckhaut, Venessa Eeckhout, Mia De Saeger, Sarah Hessenberger, Sabine Martel, An Croubels, Siska PLoS One Research Article Both mycotoxin contamination of feed and Clostridium perfringens-induced necrotic enteritis have an increasing global economic impact on poultry production. Especially the Fusarium mycotoxin deoxynivalenol (DON) is a common feed contaminant. This study aimed at examining the predisposing effect of DON on the development of necrotic enteritis in broiler chickens. An experimental Clostridium perfringens infection study revealed that DON, at a contamination level of 3,000 to 4,000 µg/kg feed, increased the percentage of birds with subclinical necrotic enteritis from 20±2.6% to 47±3.0% (P<0.001). DON significantly reduced the transepithelial electrical resistance in duodenal segments (P<0.001) and decreased duodenal villus height (P = 0.014) indicating intestinal barrier disruption and intestinal epithelial damage, respectively. This may lead to an increased permeability of the intestinal epithelium and decreased absorption of dietary proteins. Protein analysis of duodenal content indeed showed that DON contamination resulted in a significant increase in total protein concentration (P = 0.023). Furthermore, DON had no effect on in vitro growth, alpha toxin production and netB toxin transcription of Clostridium perfringens. In conclusion, feed contamination with DON at concentrations below the European maximum guidance level of 5,000 µg/kg feed, is a predisposing factor for the development of necrotic enteritis in broilers. These results are associated with a negative effect of DON on the intestinal barrier function and increased intestinal protein availability, which may stimulate growth and toxin production of Clostridium perfringens. Public Library of Science 2014-09-30 /pmc/articles/PMC4182565/ /pubmed/25268498 http://dx.doi.org/10.1371/journal.pone.0108775 Text en © 2014 Antonissen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Antonissen, Gunther Van Immerseel, Filip Pasmans, Frank Ducatelle, Richard Haesebrouck, Freddy Timbermont, Leen Verlinden, Marc Janssens, Geert Paul Jules Eeckhaut, Venessa Eeckhout, Mia De Saeger, Sarah Hessenberger, Sabine Martel, An Croubels, Siska The Mycotoxin Deoxynivalenol Predisposes for the Development of Clostridium perfringens-Induced Necrotic Enteritis in Broiler Chickens |
title | The Mycotoxin Deoxynivalenol Predisposes for the Development of Clostridium perfringens-Induced Necrotic Enteritis in Broiler Chickens |
title_full | The Mycotoxin Deoxynivalenol Predisposes for the Development of Clostridium perfringens-Induced Necrotic Enteritis in Broiler Chickens |
title_fullStr | The Mycotoxin Deoxynivalenol Predisposes for the Development of Clostridium perfringens-Induced Necrotic Enteritis in Broiler Chickens |
title_full_unstemmed | The Mycotoxin Deoxynivalenol Predisposes for the Development of Clostridium perfringens-Induced Necrotic Enteritis in Broiler Chickens |
title_short | The Mycotoxin Deoxynivalenol Predisposes for the Development of Clostridium perfringens-Induced Necrotic Enteritis in Broiler Chickens |
title_sort | mycotoxin deoxynivalenol predisposes for the development of clostridium perfringens-induced necrotic enteritis in broiler chickens |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4182565/ https://www.ncbi.nlm.nih.gov/pubmed/25268498 http://dx.doi.org/10.1371/journal.pone.0108775 |
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