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Effect of β-lactam antibiotics on plant regeneration in carrot protoplast cultures
Protoplasts of three carrot cultivars were isolated from in vitro-grown plantlets by overnight incubation in an enzyme mixture composed of 1% (w/v) cellulase Onozuka R-10 and 0.1% (w/v) pectolyase Y-23. After cell immobilization in modified thin alginate layers, three types of β-lactam antibiotics (...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Springer US
2014
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4182649/ https://www.ncbi.nlm.nih.gov/pubmed/25298730 http://dx.doi.org/10.1007/s11627-014-9626-0 |