Chimeric NP Non Coding Regions between Type A and C Influenza Viruses Reveal Their Role in Translation Regulation

Exchange of the non coding regions of the NP segment between type A and C influenza viruses was used to demonstrate the importance not only of the proximal panhandle, but also of the initial distal panhandle strength in type specificity. Both elements were found to be compulsory to rescue infectious...

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Autores principales: Crescenzo-Chaigne, Bernadette, Barbezange, Cyril, Frigard, Vianney, Poulain, Damien, van der Werf, Sylvie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4182659/
https://www.ncbi.nlm.nih.gov/pubmed/25268971
http://dx.doi.org/10.1371/journal.pone.0109046
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author Crescenzo-Chaigne, Bernadette
Barbezange, Cyril
Frigard, Vianney
Poulain, Damien
van der Werf, Sylvie
author_facet Crescenzo-Chaigne, Bernadette
Barbezange, Cyril
Frigard, Vianney
Poulain, Damien
van der Werf, Sylvie
author_sort Crescenzo-Chaigne, Bernadette
collection PubMed
description Exchange of the non coding regions of the NP segment between type A and C influenza viruses was used to demonstrate the importance not only of the proximal panhandle, but also of the initial distal panhandle strength in type specificity. Both elements were found to be compulsory to rescue infectious virus by reverse genetics systems. Interestingly, in type A influenza virus infectious context, the length of the NP segment 5′ NC region once transcribed into mRNA was found to impact its translation, and the level of produced NP protein consequently affected the level of viral genome replication.
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spelling pubmed-41826592014-10-07 Chimeric NP Non Coding Regions between Type A and C Influenza Viruses Reveal Their Role in Translation Regulation Crescenzo-Chaigne, Bernadette Barbezange, Cyril Frigard, Vianney Poulain, Damien van der Werf, Sylvie PLoS One Research Article Exchange of the non coding regions of the NP segment between type A and C influenza viruses was used to demonstrate the importance not only of the proximal panhandle, but also of the initial distal panhandle strength in type specificity. Both elements were found to be compulsory to rescue infectious virus by reverse genetics systems. Interestingly, in type A influenza virus infectious context, the length of the NP segment 5′ NC region once transcribed into mRNA was found to impact its translation, and the level of produced NP protein consequently affected the level of viral genome replication. Public Library of Science 2014-09-30 /pmc/articles/PMC4182659/ /pubmed/25268971 http://dx.doi.org/10.1371/journal.pone.0109046 Text en © 2014 Crescenzo-Chaigne et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Crescenzo-Chaigne, Bernadette
Barbezange, Cyril
Frigard, Vianney
Poulain, Damien
van der Werf, Sylvie
Chimeric NP Non Coding Regions between Type A and C Influenza Viruses Reveal Their Role in Translation Regulation
title Chimeric NP Non Coding Regions between Type A and C Influenza Viruses Reveal Their Role in Translation Regulation
title_full Chimeric NP Non Coding Regions between Type A and C Influenza Viruses Reveal Their Role in Translation Regulation
title_fullStr Chimeric NP Non Coding Regions between Type A and C Influenza Viruses Reveal Their Role in Translation Regulation
title_full_unstemmed Chimeric NP Non Coding Regions between Type A and C Influenza Viruses Reveal Their Role in Translation Regulation
title_short Chimeric NP Non Coding Regions between Type A and C Influenza Viruses Reveal Their Role in Translation Regulation
title_sort chimeric np non coding regions between type a and c influenza viruses reveal their role in translation regulation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4182659/
https://www.ncbi.nlm.nih.gov/pubmed/25268971
http://dx.doi.org/10.1371/journal.pone.0109046
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