Cargando…
Pentachlorophenol Degradation by Janibacter sp., a New Actinobacterium Isolated from Saline Sediment of Arid Land
Many pentachlorophenol- (PCP-) contaminated environments are characterized by low or elevated temperatures, acidic or alkaline pH, and high salt concentrations. PCP-degrading microorganisms, adapted to grow and prosper in these environments, play an important role in the biological treatment of poll...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4182692/ https://www.ncbi.nlm.nih.gov/pubmed/25313357 http://dx.doi.org/10.1155/2014/296472 |
_version_ | 1782337586383028224 |
---|---|
author | Khessairi, Amel Fhoula, Imene Jaouani, Atef Turki, Yousra Cherif, Ameur Boudabous, Abdellatif Hassen, Abdennaceur Ouzari, Hadda |
author_facet | Khessairi, Amel Fhoula, Imene Jaouani, Atef Turki, Yousra Cherif, Ameur Boudabous, Abdellatif Hassen, Abdennaceur Ouzari, Hadda |
author_sort | Khessairi, Amel |
collection | PubMed |
description | Many pentachlorophenol- (PCP-) contaminated environments are characterized by low or elevated temperatures, acidic or alkaline pH, and high salt concentrations. PCP-degrading microorganisms, adapted to grow and prosper in these environments, play an important role in the biological treatment of polluted extreme habitats. A PCP-degrading bacterium was isolated and characterized from arid and saline soil in southern Tunisia and was enriched in mineral salts medium supplemented with PCP as source of carbon and energy. Based on 16S rRNA coding gene sequence analysis, the strain FAS23 was identified as Janibacter sp. As revealed by high performance liquid chromatography (HPLC) analysis, FAS23 strain was found to be efficient for PCP removal in the presence of 1% of glucose. The conditions of growth and PCP removal by FAS23 strain were found to be optimal in neutral pH and at a temperature of 30°C. Moreover, this strain was found to be halotolerant at a range of 1–10% of NaCl and able to degrade PCP at a concentration up to 300 mg/L, while the addition of nonionic surfactant (Tween 80) enhanced the PCP removal capacity. |
format | Online Article Text |
id | pubmed-4182692 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-41826922014-10-13 Pentachlorophenol Degradation by Janibacter sp., a New Actinobacterium Isolated from Saline Sediment of Arid Land Khessairi, Amel Fhoula, Imene Jaouani, Atef Turki, Yousra Cherif, Ameur Boudabous, Abdellatif Hassen, Abdennaceur Ouzari, Hadda Biomed Res Int Research Article Many pentachlorophenol- (PCP-) contaminated environments are characterized by low or elevated temperatures, acidic or alkaline pH, and high salt concentrations. PCP-degrading microorganisms, adapted to grow and prosper in these environments, play an important role in the biological treatment of polluted extreme habitats. A PCP-degrading bacterium was isolated and characterized from arid and saline soil in southern Tunisia and was enriched in mineral salts medium supplemented with PCP as source of carbon and energy. Based on 16S rRNA coding gene sequence analysis, the strain FAS23 was identified as Janibacter sp. As revealed by high performance liquid chromatography (HPLC) analysis, FAS23 strain was found to be efficient for PCP removal in the presence of 1% of glucose. The conditions of growth and PCP removal by FAS23 strain were found to be optimal in neutral pH and at a temperature of 30°C. Moreover, this strain was found to be halotolerant at a range of 1–10% of NaCl and able to degrade PCP at a concentration up to 300 mg/L, while the addition of nonionic surfactant (Tween 80) enhanced the PCP removal capacity. Hindawi Publishing Corporation 2014 2014-09-17 /pmc/articles/PMC4182692/ /pubmed/25313357 http://dx.doi.org/10.1155/2014/296472 Text en Copyright © 2014 Amel Khessairi et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Khessairi, Amel Fhoula, Imene Jaouani, Atef Turki, Yousra Cherif, Ameur Boudabous, Abdellatif Hassen, Abdennaceur Ouzari, Hadda Pentachlorophenol Degradation by Janibacter sp., a New Actinobacterium Isolated from Saline Sediment of Arid Land |
title | Pentachlorophenol Degradation by Janibacter sp., a New Actinobacterium Isolated from Saline Sediment of Arid Land |
title_full | Pentachlorophenol Degradation by Janibacter sp., a New Actinobacterium Isolated from Saline Sediment of Arid Land |
title_fullStr | Pentachlorophenol Degradation by Janibacter sp., a New Actinobacterium Isolated from Saline Sediment of Arid Land |
title_full_unstemmed | Pentachlorophenol Degradation by Janibacter sp., a New Actinobacterium Isolated from Saline Sediment of Arid Land |
title_short | Pentachlorophenol Degradation by Janibacter sp., a New Actinobacterium Isolated from Saline Sediment of Arid Land |
title_sort | pentachlorophenol degradation by janibacter sp., a new actinobacterium isolated from saline sediment of arid land |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4182692/ https://www.ncbi.nlm.nih.gov/pubmed/25313357 http://dx.doi.org/10.1155/2014/296472 |
work_keys_str_mv | AT khessairiamel pentachlorophenoldegradationbyjanibacterspanewactinobacteriumisolatedfromsalinesedimentofaridland AT fhoulaimene pentachlorophenoldegradationbyjanibacterspanewactinobacteriumisolatedfromsalinesedimentofaridland AT jaouaniatef pentachlorophenoldegradationbyjanibacterspanewactinobacteriumisolatedfromsalinesedimentofaridland AT turkiyousra pentachlorophenoldegradationbyjanibacterspanewactinobacteriumisolatedfromsalinesedimentofaridland AT cherifameur pentachlorophenoldegradationbyjanibacterspanewactinobacteriumisolatedfromsalinesedimentofaridland AT boudabousabdellatif pentachlorophenoldegradationbyjanibacterspanewactinobacteriumisolatedfromsalinesedimentofaridland AT hassenabdennaceur pentachlorophenoldegradationbyjanibacterspanewactinobacteriumisolatedfromsalinesedimentofaridland AT ouzarihadda pentachlorophenoldegradationbyjanibacterspanewactinobacteriumisolatedfromsalinesedimentofaridland |