Peroxidase-Like Catalytic Activity of Ag(3)PO(4) Nanocrystals Prepared by a Colloidal Route

Nearly monodispersed Ag(3)PO(4) nanocrystals with size of 10 nm were prepared through a colloidal chemical route. It was proven that the synthesized Ag(3)PO(4) nanoparticles have intrinsic peroxidase-like catalytic activity. They can quickly catalyze oxidation of the peroxidase substrate 3, 3, 5, 5-tetramethylbenzidine (TMB) in the presence of H(2)O(2), producing a blue color. The catalysis reaction follows Michaelis-Menten kinetics. The calculated kinetic parameters indicate a high catalytic activity and the strong affinity of Ag(3)PO(4) nanocrystals to the substrate (TMB). These results suggest the potential applications of Ag(3)PO(4) nanocrystals in fields such as biotechnology, environmental chemistry, and medicine.