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Timing of high-intensity pulses for myocardial cavitation-enabled therapy
BACKGROUND: High-intensity ultrasound pulses intermittently triggered from an ECG signal can interact with circulating contrast agent microbubbles to produce myocardial cavitation microlesions of potential therapeutic value. In this study, the timing of therapy pulses relative to the ECG R wave was...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4183070/ https://www.ncbi.nlm.nih.gov/pubmed/25279221 http://dx.doi.org/10.1186/2050-5736-2-20 |
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author | Miller, Douglas L Dou, Chunyan Owens, Gabe E Kripfgans, Oliver D |
author_facet | Miller, Douglas L Dou, Chunyan Owens, Gabe E Kripfgans, Oliver D |
author_sort | Miller, Douglas L |
collection | PubMed |
description | BACKGROUND: High-intensity ultrasound pulses intermittently triggered from an ECG signal can interact with circulating contrast agent microbubbles to produce myocardial cavitation microlesions of potential therapeutic value. In this study, the timing of therapy pulses relative to the ECG R wave was investigated to identify the optimal time point for tissue reduction therapy with regard to both the physiological cardiac response and microlesion production. METHODS: Rats were anesthetized, prepared for ultrasound, placed in a heated water bath, and treated with 1.5 MHz focused ultrasound pulses targeted to the left ventricular myocardium with an 8 MHz imaging transducer. Initially, the rats were treated for 1 min at each of six different time points in the ECG while monitoring blood pressure responses to assess cardiac functional effects. Next, groups of rats were treated at three different time points: end diastole, end systole, and mid-diastole to assess the impact of timing on microlesion creation. These rats were pretreated with Evans blue injections and were allowed to recover for 1 day until hearts were harvested for scoring of injured cardiomyocytes. RESULTS: The initial results showed a wide range of cardiac premature complexes in the ECG, which corresponded with blood pressure pulses for ultrasound pulses triggered during diastole. However, the microlesion experiment did not reveal any statistically significant variations in cardiomyocyte injury. CONCLUSION: The end of systole (R + RR/3) was identified as an optimal trigger time point which produced identifiable ECG complexes and substantial cardiomyocyte injury but minimal cardiac functional disruption during treatment. |
format | Online Article Text |
id | pubmed-4183070 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-41830702014-10-03 Timing of high-intensity pulses for myocardial cavitation-enabled therapy Miller, Douglas L Dou, Chunyan Owens, Gabe E Kripfgans, Oliver D J Ther Ultrasound Research BACKGROUND: High-intensity ultrasound pulses intermittently triggered from an ECG signal can interact with circulating contrast agent microbubbles to produce myocardial cavitation microlesions of potential therapeutic value. In this study, the timing of therapy pulses relative to the ECG R wave was investigated to identify the optimal time point for tissue reduction therapy with regard to both the physiological cardiac response and microlesion production. METHODS: Rats were anesthetized, prepared for ultrasound, placed in a heated water bath, and treated with 1.5 MHz focused ultrasound pulses targeted to the left ventricular myocardium with an 8 MHz imaging transducer. Initially, the rats were treated for 1 min at each of six different time points in the ECG while monitoring blood pressure responses to assess cardiac functional effects. Next, groups of rats were treated at three different time points: end diastole, end systole, and mid-diastole to assess the impact of timing on microlesion creation. These rats were pretreated with Evans blue injections and were allowed to recover for 1 day until hearts were harvested for scoring of injured cardiomyocytes. RESULTS: The initial results showed a wide range of cardiac premature complexes in the ECG, which corresponded with blood pressure pulses for ultrasound pulses triggered during diastole. However, the microlesion experiment did not reveal any statistically significant variations in cardiomyocyte injury. CONCLUSION: The end of systole (R + RR/3) was identified as an optimal trigger time point which produced identifiable ECG complexes and substantial cardiomyocyte injury but minimal cardiac functional disruption during treatment. BioMed Central 2014-10-02 /pmc/articles/PMC4183070/ /pubmed/25279221 http://dx.doi.org/10.1186/2050-5736-2-20 Text en Copyright © 2014 Miller et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Miller, Douglas L Dou, Chunyan Owens, Gabe E Kripfgans, Oliver D Timing of high-intensity pulses for myocardial cavitation-enabled therapy |
title | Timing of high-intensity pulses for myocardial cavitation-enabled therapy |
title_full | Timing of high-intensity pulses for myocardial cavitation-enabled therapy |
title_fullStr | Timing of high-intensity pulses for myocardial cavitation-enabled therapy |
title_full_unstemmed | Timing of high-intensity pulses for myocardial cavitation-enabled therapy |
title_short | Timing of high-intensity pulses for myocardial cavitation-enabled therapy |
title_sort | timing of high-intensity pulses for myocardial cavitation-enabled therapy |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4183070/ https://www.ncbi.nlm.nih.gov/pubmed/25279221 http://dx.doi.org/10.1186/2050-5736-2-20 |
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