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Effect of low-level laser therapy on the modulation of the mitochondrial activity of macrophages

BACKGROUND: Macrophages play a major role among the inflammatory cells that invade muscle tissue following an injury. Low-level laser therapy (LLLT) has long been used in clinical practice to accelerate the muscle repair process. However, little is known regarding its effect on macrophages. OBJECTIV...

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Autores principales: Souza, Nadhia H. C., Ferrari, Raquel A. M., Silva, Daniela F. T., Nunes, Fabio D., Bussadori, Sandra K., Fernandes, Kristianne P. S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Associação Brasileira de Pesquisa e Pós-Graduação em Fisioterapia 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4183262/
https://www.ncbi.nlm.nih.gov/pubmed/25076002
http://dx.doi.org/10.1590/bjpt-rbf.2014.0046
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author Souza, Nadhia H. C.
Ferrari, Raquel A. M.
Silva, Daniela F. T.
Nunes, Fabio D.
Bussadori, Sandra K.
Fernandes, Kristianne P. S.
author_facet Souza, Nadhia H. C.
Ferrari, Raquel A. M.
Silva, Daniela F. T.
Nunes, Fabio D.
Bussadori, Sandra K.
Fernandes, Kristianne P. S.
author_sort Souza, Nadhia H. C.
collection PubMed
description BACKGROUND: Macrophages play a major role among the inflammatory cells that invade muscle tissue following an injury. Low-level laser therapy (LLLT) has long been used in clinical practice to accelerate the muscle repair process. However, little is known regarding its effect on macrophages. OBJECTIVE: This study evaluated the effect of LLLT on the mitochondrial activity (MA) of macrophages. METHOD: J774 macrophages were treated with lipopolysaccharide (LPS) and interferon - gamma (IFN-γ) (activation) for 24 h to simulate an inflammatory process, then irradiated with LLLT using two sets of parameters (780 nm; 70 mW; 3 J/cm(2) and 660 nm; 15 mW; 7.5 J/cm(2)). Non-activated/non-irradiated cells composed the control group. MA was evaluated by the cell mitochondrial activity (MTT) assay (after 1, 3 and 5 days) in three independent experiments. The data were analyzed statistically. RESULTS: After 1 day of culture, activated and 780 nm irradiated macrophages showed lower MA than activated macrophages, but activated and 660 nm irradiated macrophages showed MA similar to activated cells. After 3 days, activated and irradiated (660 nm and 780 nm) macrophages showed greater MA than activated macrophages, and after 5 days, the activated and irradiated (660 nm and 780 nm) macrophages showed similar MA to the activated macrophages. CONCLUSIONS: These results show that 660 nm and 780 nm LLLT can modulate the cellular activation status of macrophages in inflammation, highlighting the importance of this resource and of the correct determination of its parameters in the repair process of skeletal muscle.
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spelling pubmed-41832622014-10-23 Effect of low-level laser therapy on the modulation of the mitochondrial activity of macrophages Souza, Nadhia H. C. Ferrari, Raquel A. M. Silva, Daniela F. T. Nunes, Fabio D. Bussadori, Sandra K. Fernandes, Kristianne P. S. Braz J Phys Ther Original Articles BACKGROUND: Macrophages play a major role among the inflammatory cells that invade muscle tissue following an injury. Low-level laser therapy (LLLT) has long been used in clinical practice to accelerate the muscle repair process. However, little is known regarding its effect on macrophages. OBJECTIVE: This study evaluated the effect of LLLT on the mitochondrial activity (MA) of macrophages. METHOD: J774 macrophages were treated with lipopolysaccharide (LPS) and interferon - gamma (IFN-γ) (activation) for 24 h to simulate an inflammatory process, then irradiated with LLLT using two sets of parameters (780 nm; 70 mW; 3 J/cm(2) and 660 nm; 15 mW; 7.5 J/cm(2)). Non-activated/non-irradiated cells composed the control group. MA was evaluated by the cell mitochondrial activity (MTT) assay (after 1, 3 and 5 days) in three independent experiments. The data were analyzed statistically. RESULTS: After 1 day of culture, activated and 780 nm irradiated macrophages showed lower MA than activated macrophages, but activated and 660 nm irradiated macrophages showed MA similar to activated cells. After 3 days, activated and irradiated (660 nm and 780 nm) macrophages showed greater MA than activated macrophages, and after 5 days, the activated and irradiated (660 nm and 780 nm) macrophages showed similar MA to the activated macrophages. CONCLUSIONS: These results show that 660 nm and 780 nm LLLT can modulate the cellular activation status of macrophages in inflammation, highlighting the importance of this resource and of the correct determination of its parameters in the repair process of skeletal muscle. Associação Brasileira de Pesquisa e Pós-Graduação em Fisioterapia 2014 /pmc/articles/PMC4183262/ /pubmed/25076002 http://dx.doi.org/10.1590/bjpt-rbf.2014.0046 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Souza, Nadhia H. C.
Ferrari, Raquel A. M.
Silva, Daniela F. T.
Nunes, Fabio D.
Bussadori, Sandra K.
Fernandes, Kristianne P. S.
Effect of low-level laser therapy on the modulation of the mitochondrial activity of macrophages
title Effect of low-level laser therapy on the modulation of the mitochondrial activity of macrophages
title_full Effect of low-level laser therapy on the modulation of the mitochondrial activity of macrophages
title_fullStr Effect of low-level laser therapy on the modulation of the mitochondrial activity of macrophages
title_full_unstemmed Effect of low-level laser therapy on the modulation of the mitochondrial activity of macrophages
title_short Effect of low-level laser therapy on the modulation of the mitochondrial activity of macrophages
title_sort effect of low-level laser therapy on the modulation of the mitochondrial activity of macrophages
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4183262/
https://www.ncbi.nlm.nih.gov/pubmed/25076002
http://dx.doi.org/10.1590/bjpt-rbf.2014.0046
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