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Synergism and Antagonism between Bacillus thuringiensis Vip3A and Cry1 Proteins in Heliothis virescens, Diatraea saccharalis and Spodoptera frugiperda
Second generation Bt crops (insect resistant crops carrying Bacillus thuringiensis genes) combine more than one gene that codes for insecticidal proteins in the same plant to provide better control of agricultural pests. Some of the new combinations involve co-expression of cry and vip genes. Becaus...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4183464/ https://www.ncbi.nlm.nih.gov/pubmed/25275646 http://dx.doi.org/10.1371/journal.pone.0107196 |
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author | Lemes, Ana Rita Nunes Davolos, Camila Chiaradia Legori, Paula Cristina Brunini Crialesi Fernandes, Odair Aparecido Ferré, Juan Lemos, Manoel Victor Franco Desiderio, Janete Apparecida |
author_facet | Lemes, Ana Rita Nunes Davolos, Camila Chiaradia Legori, Paula Cristina Brunini Crialesi Fernandes, Odair Aparecido Ferré, Juan Lemos, Manoel Victor Franco Desiderio, Janete Apparecida |
author_sort | Lemes, Ana Rita Nunes |
collection | PubMed |
description | Second generation Bt crops (insect resistant crops carrying Bacillus thuringiensis genes) combine more than one gene that codes for insecticidal proteins in the same plant to provide better control of agricultural pests. Some of the new combinations involve co-expression of cry and vip genes. Because Cry and Vip proteins have different midgut targets and possibly different mechanisms of toxicity, it is important to evaluate possible synergistic or antagonistic interactions between these two classes of toxins. Three members of the Cry1 class of proteins and three from the Vip3A class were tested against Heliothis virescens for possible interactions. At the level of LC(50), Cry1Ac was the most active protein, whereas the rest of proteins tested were similarly active. However, at the level of LC(90), Cry1Aa and Cry1Ca were the least active proteins, and Cry1Ac and Vip3A proteins were not significantly different. Under the experimental conditions used in this study, we found an antagonistic effect of Cry1Ca with the three Vip3A proteins. The interaction between Cry1Ca and Vip3Aa was also tested on two other species of Lepidoptera. Whereas antagonism was observed in Spodoptera frugiperda, synergism was found in Diatraea saccharalis. In all cases, the interaction between Vip3A and Cry1 proteins was more evident at the LC(90) level than at the LC(50) level. The fact that the same combination of proteins may result in a synergistic or an antagonistic interaction may be an indication that there are different types of interactions within the host, depending on the insect species tested. |
format | Online Article Text |
id | pubmed-4183464 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41834642014-10-07 Synergism and Antagonism between Bacillus thuringiensis Vip3A and Cry1 Proteins in Heliothis virescens, Diatraea saccharalis and Spodoptera frugiperda Lemes, Ana Rita Nunes Davolos, Camila Chiaradia Legori, Paula Cristina Brunini Crialesi Fernandes, Odair Aparecido Ferré, Juan Lemos, Manoel Victor Franco Desiderio, Janete Apparecida PLoS One Research Article Second generation Bt crops (insect resistant crops carrying Bacillus thuringiensis genes) combine more than one gene that codes for insecticidal proteins in the same plant to provide better control of agricultural pests. Some of the new combinations involve co-expression of cry and vip genes. Because Cry and Vip proteins have different midgut targets and possibly different mechanisms of toxicity, it is important to evaluate possible synergistic or antagonistic interactions between these two classes of toxins. Three members of the Cry1 class of proteins and three from the Vip3A class were tested against Heliothis virescens for possible interactions. At the level of LC(50), Cry1Ac was the most active protein, whereas the rest of proteins tested were similarly active. However, at the level of LC(90), Cry1Aa and Cry1Ca were the least active proteins, and Cry1Ac and Vip3A proteins were not significantly different. Under the experimental conditions used in this study, we found an antagonistic effect of Cry1Ca with the three Vip3A proteins. The interaction between Cry1Ca and Vip3Aa was also tested on two other species of Lepidoptera. Whereas antagonism was observed in Spodoptera frugiperda, synergism was found in Diatraea saccharalis. In all cases, the interaction between Vip3A and Cry1 proteins was more evident at the LC(90) level than at the LC(50) level. The fact that the same combination of proteins may result in a synergistic or an antagonistic interaction may be an indication that there are different types of interactions within the host, depending on the insect species tested. Public Library of Science 2014-10-02 /pmc/articles/PMC4183464/ /pubmed/25275646 http://dx.doi.org/10.1371/journal.pone.0107196 Text en © 2014 Lemes et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Lemes, Ana Rita Nunes Davolos, Camila Chiaradia Legori, Paula Cristina Brunini Crialesi Fernandes, Odair Aparecido Ferré, Juan Lemos, Manoel Victor Franco Desiderio, Janete Apparecida Synergism and Antagonism between Bacillus thuringiensis Vip3A and Cry1 Proteins in Heliothis virescens, Diatraea saccharalis and Spodoptera frugiperda |
title | Synergism and Antagonism between Bacillus thuringiensis Vip3A and Cry1 Proteins in Heliothis virescens, Diatraea saccharalis and Spodoptera frugiperda
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title_full | Synergism and Antagonism between Bacillus thuringiensis Vip3A and Cry1 Proteins in Heliothis virescens, Diatraea saccharalis and Spodoptera frugiperda
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title_fullStr | Synergism and Antagonism between Bacillus thuringiensis Vip3A and Cry1 Proteins in Heliothis virescens, Diatraea saccharalis and Spodoptera frugiperda
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title_full_unstemmed | Synergism and Antagonism between Bacillus thuringiensis Vip3A and Cry1 Proteins in Heliothis virescens, Diatraea saccharalis and Spodoptera frugiperda
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title_short | Synergism and Antagonism between Bacillus thuringiensis Vip3A and Cry1 Proteins in Heliothis virescens, Diatraea saccharalis and Spodoptera frugiperda
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title_sort | synergism and antagonism between bacillus thuringiensis vip3a and cry1 proteins in heliothis virescens, diatraea saccharalis and spodoptera frugiperda |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4183464/ https://www.ncbi.nlm.nih.gov/pubmed/25275646 http://dx.doi.org/10.1371/journal.pone.0107196 |
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