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Molecular Epidemiology of Japanese Encephalitis Virus in Mosquitoes in Taiwan during 2005–2012

Japanese encephalitis (JE) is a mosquito-borne zoonotic disease caused by the Japanese encephalitis virus (JEV). Pigs and water birds are the main amplifying and maintenance hosts of the virus. In this study, we conducted a JEV survey in mosquitoes captured in pig farms and water bird wetland habita...

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Autores principales: Su, Chien-Ling, Yang, Cheng-Fen, Teng, Hwa-Jen, Lu, Liang-Chen, Lin, Cheo, Tsai, Kun-Hsien, Chen, Yu-Yu, Chen, Li-Yu, Chang, Shu-Fen, Shu, Pei-Yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4183467/
https://www.ncbi.nlm.nih.gov/pubmed/25275652
http://dx.doi.org/10.1371/journal.pntd.0003122
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author Su, Chien-Ling
Yang, Cheng-Fen
Teng, Hwa-Jen
Lu, Liang-Chen
Lin, Cheo
Tsai, Kun-Hsien
Chen, Yu-Yu
Chen, Li-Yu
Chang, Shu-Fen
Shu, Pei-Yun
author_facet Su, Chien-Ling
Yang, Cheng-Fen
Teng, Hwa-Jen
Lu, Liang-Chen
Lin, Cheo
Tsai, Kun-Hsien
Chen, Yu-Yu
Chen, Li-Yu
Chang, Shu-Fen
Shu, Pei-Yun
author_sort Su, Chien-Ling
collection PubMed
description Japanese encephalitis (JE) is a mosquito-borne zoonotic disease caused by the Japanese encephalitis virus (JEV). Pigs and water birds are the main amplifying and maintenance hosts of the virus. In this study, we conducted a JEV survey in mosquitoes captured in pig farms and water bird wetland habitats in Taiwan during 2005 to 2012. A total of 102,633 mosquitoes were collected. Culex tritaeniorhynchus was the most common mosquito species found in the pig farms and wetlands. Among the 26 mosquito species collected, 11 tested positive for JEV by RT-PCR, including Cx. tritaeniorhynchus, Cx. annulus, Anopheles sinensis, Armigeres subalbatus, and Cx. fuscocephala. Among those testing positive, Cx. tritaeniorhynchus was the predominant vector species for the transmission of JEV genotypes I and III in Taiwan. The JEV infection rate was significantly higher in the mosquitoes from the pig farms than those from the wetlands. A phylogenetic analysis of the JEV envelope gene sequences isolated from the captured mosquitoes demonstrated that the predominant JEV genotype has shifted from genotype III to genotype I (GI), providing evidence for transmission cycle maintenance and multiple introductions of the GI strains in Taiwan during 2008 to 2012. This study demonstrates the intense JEV transmission activity in Taiwan, highlights the importance of JE vaccination for controlling the epidemic, and provides valuable information for the assessment of the vaccine's efficacy.
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spelling pubmed-41834672014-10-07 Molecular Epidemiology of Japanese Encephalitis Virus in Mosquitoes in Taiwan during 2005–2012 Su, Chien-Ling Yang, Cheng-Fen Teng, Hwa-Jen Lu, Liang-Chen Lin, Cheo Tsai, Kun-Hsien Chen, Yu-Yu Chen, Li-Yu Chang, Shu-Fen Shu, Pei-Yun PLoS Negl Trop Dis Research Article Japanese encephalitis (JE) is a mosquito-borne zoonotic disease caused by the Japanese encephalitis virus (JEV). Pigs and water birds are the main amplifying and maintenance hosts of the virus. In this study, we conducted a JEV survey in mosquitoes captured in pig farms and water bird wetland habitats in Taiwan during 2005 to 2012. A total of 102,633 mosquitoes were collected. Culex tritaeniorhynchus was the most common mosquito species found in the pig farms and wetlands. Among the 26 mosquito species collected, 11 tested positive for JEV by RT-PCR, including Cx. tritaeniorhynchus, Cx. annulus, Anopheles sinensis, Armigeres subalbatus, and Cx. fuscocephala. Among those testing positive, Cx. tritaeniorhynchus was the predominant vector species for the transmission of JEV genotypes I and III in Taiwan. The JEV infection rate was significantly higher in the mosquitoes from the pig farms than those from the wetlands. A phylogenetic analysis of the JEV envelope gene sequences isolated from the captured mosquitoes demonstrated that the predominant JEV genotype has shifted from genotype III to genotype I (GI), providing evidence for transmission cycle maintenance and multiple introductions of the GI strains in Taiwan during 2008 to 2012. This study demonstrates the intense JEV transmission activity in Taiwan, highlights the importance of JE vaccination for controlling the epidemic, and provides valuable information for the assessment of the vaccine's efficacy. Public Library of Science 2014-10-02 /pmc/articles/PMC4183467/ /pubmed/25275652 http://dx.doi.org/10.1371/journal.pntd.0003122 Text en © 2014 Su et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Su, Chien-Ling
Yang, Cheng-Fen
Teng, Hwa-Jen
Lu, Liang-Chen
Lin, Cheo
Tsai, Kun-Hsien
Chen, Yu-Yu
Chen, Li-Yu
Chang, Shu-Fen
Shu, Pei-Yun
Molecular Epidemiology of Japanese Encephalitis Virus in Mosquitoes in Taiwan during 2005–2012
title Molecular Epidemiology of Japanese Encephalitis Virus in Mosquitoes in Taiwan during 2005–2012
title_full Molecular Epidemiology of Japanese Encephalitis Virus in Mosquitoes in Taiwan during 2005–2012
title_fullStr Molecular Epidemiology of Japanese Encephalitis Virus in Mosquitoes in Taiwan during 2005–2012
title_full_unstemmed Molecular Epidemiology of Japanese Encephalitis Virus in Mosquitoes in Taiwan during 2005–2012
title_short Molecular Epidemiology of Japanese Encephalitis Virus in Mosquitoes in Taiwan during 2005–2012
title_sort molecular epidemiology of japanese encephalitis virus in mosquitoes in taiwan during 2005–2012
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4183467/
https://www.ncbi.nlm.nih.gov/pubmed/25275652
http://dx.doi.org/10.1371/journal.pntd.0003122
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