Cargando…
Mycobacterium bovis in Burkina Faso: Epidemiologic and Genetic Links between Human and Cattle Isolates
BACKGROUND: In sub-Saharan Africa, bovine tuberculosis (bTB) is a potential hazard for animals and humans health. The goal of this study was to improve our understanding of bTB epidemiology in Burkina Faso and especially Mycobacterium bovis transmission within and between the bovine and human popula...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4183478/ https://www.ncbi.nlm.nih.gov/pubmed/25275305 http://dx.doi.org/10.1371/journal.pntd.0003142 |
_version_ | 1782337694614945792 |
---|---|
author | Sanou, Adama Tarnagda, Zekiba Kanyala, Estelle Zingué, Dezemon Nouctara, Moumini Ganamé, Zakaria Combary, Adjima Hien, Hervé Dembele, Mathurin Kabore, Antoinette Meda, Nicolas Van de Perre, Philippe Neveu, Dorine Bañuls, Anne Laure Godreuil, Sylvain |
author_facet | Sanou, Adama Tarnagda, Zekiba Kanyala, Estelle Zingué, Dezemon Nouctara, Moumini Ganamé, Zakaria Combary, Adjima Hien, Hervé Dembele, Mathurin Kabore, Antoinette Meda, Nicolas Van de Perre, Philippe Neveu, Dorine Bañuls, Anne Laure Godreuil, Sylvain |
author_sort | Sanou, Adama |
collection | PubMed |
description | BACKGROUND: In sub-Saharan Africa, bovine tuberculosis (bTB) is a potential hazard for animals and humans health. The goal of this study was to improve our understanding of bTB epidemiology in Burkina Faso and especially Mycobacterium bovis transmission within and between the bovine and human populations. METHODOLOGY/PRINCIPAL FINDINGS: Twenty six M. bovis strains were isolated from 101 cattle carcasses with suspected bTB lesions during routine meat inspections at the Bobo Dioulasso and Ouagadougou slaughterhouses. In addition, 7 M. bovis strains were isolated from 576 patients with pulmonary tuberculosis. Spoligotyping, RDAf1 deletion and MIRU-VNTR typing were used for strains genotyping. The isolation of M. bovis strains was confirmed by spoligotyping and 12 spoligotype signatures were detected. Together, the spoligotyping and MIRU-VNTR data allowed grouping the 33 M. bovis isolates in seven clusters including isolates exclusively from cattle (5) or humans (1) or from both (1). Moreover, these data (genetic analyses and phenetic tree) showed that the M. bovis isolates belonged to the African 1 (Af1) clonal complex (81.8%) and the putative African 5 (Af5) clonal complex (18.2%), in agreement with the results of RDAf1 deletion typing. CONCLUSIONS/SIGNIFICANCE: This is the first detailed molecular characterization of M. bovis strains from humans and cattle in Burkina Faso. The distribution of the two Af1 and putative Af5 clonal complexes is comparable to what has been reported in neighbouring countries. Furthermore, the strain genetic profiles suggest that M. bovis circulates across the borders and that the Burkina Faso strains originate from different countries, but have a country-specific evolution. The genetic characterization suggests that, currently, M. bovis transmission occurs mainly between cattle, occasionally between cattle and humans and potentially between humans. This study emphasizes the bTB risk in cattle but also in humans and the difficulty to set up proper disease control strategies in Burkina Faso. |
format | Online Article Text |
id | pubmed-4183478 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41834782014-10-07 Mycobacterium bovis in Burkina Faso: Epidemiologic and Genetic Links between Human and Cattle Isolates Sanou, Adama Tarnagda, Zekiba Kanyala, Estelle Zingué, Dezemon Nouctara, Moumini Ganamé, Zakaria Combary, Adjima Hien, Hervé Dembele, Mathurin Kabore, Antoinette Meda, Nicolas Van de Perre, Philippe Neveu, Dorine Bañuls, Anne Laure Godreuil, Sylvain PLoS Negl Trop Dis Research Article BACKGROUND: In sub-Saharan Africa, bovine tuberculosis (bTB) is a potential hazard for animals and humans health. The goal of this study was to improve our understanding of bTB epidemiology in Burkina Faso and especially Mycobacterium bovis transmission within and between the bovine and human populations. METHODOLOGY/PRINCIPAL FINDINGS: Twenty six M. bovis strains were isolated from 101 cattle carcasses with suspected bTB lesions during routine meat inspections at the Bobo Dioulasso and Ouagadougou slaughterhouses. In addition, 7 M. bovis strains were isolated from 576 patients with pulmonary tuberculosis. Spoligotyping, RDAf1 deletion and MIRU-VNTR typing were used for strains genotyping. The isolation of M. bovis strains was confirmed by spoligotyping and 12 spoligotype signatures were detected. Together, the spoligotyping and MIRU-VNTR data allowed grouping the 33 M. bovis isolates in seven clusters including isolates exclusively from cattle (5) or humans (1) or from both (1). Moreover, these data (genetic analyses and phenetic tree) showed that the M. bovis isolates belonged to the African 1 (Af1) clonal complex (81.8%) and the putative African 5 (Af5) clonal complex (18.2%), in agreement with the results of RDAf1 deletion typing. CONCLUSIONS/SIGNIFICANCE: This is the first detailed molecular characterization of M. bovis strains from humans and cattle in Burkina Faso. The distribution of the two Af1 and putative Af5 clonal complexes is comparable to what has been reported in neighbouring countries. Furthermore, the strain genetic profiles suggest that M. bovis circulates across the borders and that the Burkina Faso strains originate from different countries, but have a country-specific evolution. The genetic characterization suggests that, currently, M. bovis transmission occurs mainly between cattle, occasionally between cattle and humans and potentially between humans. This study emphasizes the bTB risk in cattle but also in humans and the difficulty to set up proper disease control strategies in Burkina Faso. Public Library of Science 2014-10-02 /pmc/articles/PMC4183478/ /pubmed/25275305 http://dx.doi.org/10.1371/journal.pntd.0003142 Text en © 2014 Sanou et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Sanou, Adama Tarnagda, Zekiba Kanyala, Estelle Zingué, Dezemon Nouctara, Moumini Ganamé, Zakaria Combary, Adjima Hien, Hervé Dembele, Mathurin Kabore, Antoinette Meda, Nicolas Van de Perre, Philippe Neveu, Dorine Bañuls, Anne Laure Godreuil, Sylvain Mycobacterium bovis in Burkina Faso: Epidemiologic and Genetic Links between Human and Cattle Isolates |
title |
Mycobacterium bovis in Burkina Faso: Epidemiologic and Genetic Links between Human and Cattle Isolates |
title_full |
Mycobacterium bovis in Burkina Faso: Epidemiologic and Genetic Links between Human and Cattle Isolates |
title_fullStr |
Mycobacterium bovis in Burkina Faso: Epidemiologic and Genetic Links between Human and Cattle Isolates |
title_full_unstemmed |
Mycobacterium bovis in Burkina Faso: Epidemiologic and Genetic Links between Human and Cattle Isolates |
title_short |
Mycobacterium bovis in Burkina Faso: Epidemiologic and Genetic Links between Human and Cattle Isolates |
title_sort | mycobacterium bovis in burkina faso: epidemiologic and genetic links between human and cattle isolates |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4183478/ https://www.ncbi.nlm.nih.gov/pubmed/25275305 http://dx.doi.org/10.1371/journal.pntd.0003142 |
work_keys_str_mv | AT sanouadama mycobacteriumbovisinburkinafasoepidemiologicandgeneticlinksbetweenhumanandcattleisolates AT tarnagdazekiba mycobacteriumbovisinburkinafasoepidemiologicandgeneticlinksbetweenhumanandcattleisolates AT kanyalaestelle mycobacteriumbovisinburkinafasoepidemiologicandgeneticlinksbetweenhumanandcattleisolates AT zinguedezemon mycobacteriumbovisinburkinafasoepidemiologicandgeneticlinksbetweenhumanandcattleisolates AT nouctaramoumini mycobacteriumbovisinburkinafasoepidemiologicandgeneticlinksbetweenhumanandcattleisolates AT ganamezakaria mycobacteriumbovisinburkinafasoepidemiologicandgeneticlinksbetweenhumanandcattleisolates AT combaryadjima mycobacteriumbovisinburkinafasoepidemiologicandgeneticlinksbetweenhumanandcattleisolates AT hienherve mycobacteriumbovisinburkinafasoepidemiologicandgeneticlinksbetweenhumanandcattleisolates AT dembelemathurin mycobacteriumbovisinburkinafasoepidemiologicandgeneticlinksbetweenhumanandcattleisolates AT kaboreantoinette mycobacteriumbovisinburkinafasoepidemiologicandgeneticlinksbetweenhumanandcattleisolates AT medanicolas mycobacteriumbovisinburkinafasoepidemiologicandgeneticlinksbetweenhumanandcattleisolates AT vandeperrephilippe mycobacteriumbovisinburkinafasoepidemiologicandgeneticlinksbetweenhumanandcattleisolates AT neveudorine mycobacteriumbovisinburkinafasoepidemiologicandgeneticlinksbetweenhumanandcattleisolates AT banulsannelaure mycobacteriumbovisinburkinafasoepidemiologicandgeneticlinksbetweenhumanandcattleisolates AT godreuilsylvain mycobacteriumbovisinburkinafasoepidemiologicandgeneticlinksbetweenhumanandcattleisolates |