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Mammary stem cells have myoepithelial cell properties

Contractile myoepithelial cells dominate the basal layer of the mammary epithelium and are considered to be differentiated cells. However, we observe that up to 54% of single basal cells can form colonies when seeded into adherent culture in the presence of agents that disrupt acin-myosin interactio...

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Autores principales: Prater, Michael D., Petit, Valérie, Russell, I. Alasdair, Giraddi, Rajshekhar, Shehata, Mona, Menon, Suraj, Schulte, Reiner, Kalajzic, Ivo, Rath, Nicola, Olson, Michael F., Metzger, Daniel, Faraldo, Marisa M., Deugnier, Marie-Ange, Glukhova, Marina A., Stingl, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4183554/
https://www.ncbi.nlm.nih.gov/pubmed/25173976
http://dx.doi.org/10.1038/ncb3025
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author Prater, Michael D.
Petit, Valérie
Russell, I. Alasdair
Giraddi, Rajshekhar
Shehata, Mona
Menon, Suraj
Schulte, Reiner
Kalajzic, Ivo
Rath, Nicola
Olson, Michael F.
Metzger, Daniel
Faraldo, Marisa M.
Deugnier, Marie-Ange
Glukhova, Marina A.
Stingl, John
author_facet Prater, Michael D.
Petit, Valérie
Russell, I. Alasdair
Giraddi, Rajshekhar
Shehata, Mona
Menon, Suraj
Schulte, Reiner
Kalajzic, Ivo
Rath, Nicola
Olson, Michael F.
Metzger, Daniel
Faraldo, Marisa M.
Deugnier, Marie-Ange
Glukhova, Marina A.
Stingl, John
author_sort Prater, Michael D.
collection PubMed
description Contractile myoepithelial cells dominate the basal layer of the mammary epithelium and are considered to be differentiated cells. However, we observe that up to 54% of single basal cells can form colonies when seeded into adherent culture in the presence of agents that disrupt acin-myosin interactions, and on average, 65% of the single-cell-derived basal colonies can repopulate a mammary gland when transplanted in vivo. This indicates that a high proportion of basal myoepithelial cells can give rise to a mammary repopulating unit (MRU). We demonstrate that myoepithelial cells, flow-sorted using 2 independent myoepithelial-specific reporter strategies, have MRU capacity. Using an inducible lineage tracing approach we follow the progeny of α-smooth muscle actin-expressing myoepithelial cells and show that they function as long-lived lineage-restricted stem cells in the virgin state and during pregnancy.
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spelling pubmed-41835542015-04-01 Mammary stem cells have myoepithelial cell properties Prater, Michael D. Petit, Valérie Russell, I. Alasdair Giraddi, Rajshekhar Shehata, Mona Menon, Suraj Schulte, Reiner Kalajzic, Ivo Rath, Nicola Olson, Michael F. Metzger, Daniel Faraldo, Marisa M. Deugnier, Marie-Ange Glukhova, Marina A. Stingl, John Nat Cell Biol Article Contractile myoepithelial cells dominate the basal layer of the mammary epithelium and are considered to be differentiated cells. However, we observe that up to 54% of single basal cells can form colonies when seeded into adherent culture in the presence of agents that disrupt acin-myosin interactions, and on average, 65% of the single-cell-derived basal colonies can repopulate a mammary gland when transplanted in vivo. This indicates that a high proportion of basal myoepithelial cells can give rise to a mammary repopulating unit (MRU). We demonstrate that myoepithelial cells, flow-sorted using 2 independent myoepithelial-specific reporter strategies, have MRU capacity. Using an inducible lineage tracing approach we follow the progeny of α-smooth muscle actin-expressing myoepithelial cells and show that they function as long-lived lineage-restricted stem cells in the virgin state and during pregnancy. 2014-08-31 2014-10 /pmc/articles/PMC4183554/ /pubmed/25173976 http://dx.doi.org/10.1038/ncb3025 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Prater, Michael D.
Petit, Valérie
Russell, I. Alasdair
Giraddi, Rajshekhar
Shehata, Mona
Menon, Suraj
Schulte, Reiner
Kalajzic, Ivo
Rath, Nicola
Olson, Michael F.
Metzger, Daniel
Faraldo, Marisa M.
Deugnier, Marie-Ange
Glukhova, Marina A.
Stingl, John
Mammary stem cells have myoepithelial cell properties
title Mammary stem cells have myoepithelial cell properties
title_full Mammary stem cells have myoepithelial cell properties
title_fullStr Mammary stem cells have myoepithelial cell properties
title_full_unstemmed Mammary stem cells have myoepithelial cell properties
title_short Mammary stem cells have myoepithelial cell properties
title_sort mammary stem cells have myoepithelial cell properties
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4183554/
https://www.ncbi.nlm.nih.gov/pubmed/25173976
http://dx.doi.org/10.1038/ncb3025
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