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Hyperoxygenation Attenuated a Murine Model of Atopic Dermatitis through Raising Skin Level of ROS
Atopic dermatitis (AD) is a chronic inflammatory skin disease resulting from excessive stimulation of immune cells. Traditionally, reactive oxygen species (ROS) have been implicated in the progression of inflammatory diseases, but several opposing observations suggest the protective role of ROS in i...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4183587/ https://www.ncbi.nlm.nih.gov/pubmed/25275529 http://dx.doi.org/10.1371/journal.pone.0109297 |
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author | Kim, Hyung-Ran Kim, Jung-Hwan Choi, Eun-Jeong Lee, Yeo Kyong Kie, Jeong-Hae Jang, Myoung Ho Seoh, Ju-Young |
author_facet | Kim, Hyung-Ran Kim, Jung-Hwan Choi, Eun-Jeong Lee, Yeo Kyong Kie, Jeong-Hae Jang, Myoung Ho Seoh, Ju-Young |
author_sort | Kim, Hyung-Ran |
collection | PubMed |
description | Atopic dermatitis (AD) is a chronic inflammatory skin disease resulting from excessive stimulation of immune cells. Traditionally, reactive oxygen species (ROS) have been implicated in the progression of inflammatory diseases, but several opposing observations suggest the protective role of ROS in inflammatory disease. Recently, we demonstrated ROS prevented imiquimod-induced psoriatic dermatitis through enhancing regulatory T cell function. Thus, we hypothesized AD might also be attenuated in elevated levels of ROS through tissue hyperoxygenation, such as by hyperbaric oxygen therapy (HBOT) or applying an oxygen-carrying chemical, perfluorodecalin (PFD). Elevated levels of ROS in the skin have been demonstrated directly by staining with dihydroethidum as well as indirectly by immunohistochemistry (IHC) for indoleamine 2,3-dioxygenase (IDO). A murine model of AD was developed by repeated application of a chemical irritant (1% 2,4-dinitrochlorobenzene) and house dust mite (Dermatophagoide farinae) extract on one ear of BALB/c mice. The results showed treatment with HBOT or PFD significantly attenuated AD, comparably with 0.1% prednicarbate without any signs of side effects, such as telangiectasia. The expressions of interleukin-17A and interferon-γ were also decreased in the AD lesions by treatment with HBOT or PFD. Enhanced expression of IDO and reduced level of hypoxia-inducible factor-1α, in association with increased frequency of FoxP3(+) regulatory T cells in the AD lesions, might be involved in the underlying mechanism of oxygen therapy. Taken together, it was suggested that tissue hyperoxygenation, by HBOT or treatment with PFD, might attenuate AD through enhancing skin ROS level. |
format | Online Article Text |
id | pubmed-4183587 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41835872014-10-07 Hyperoxygenation Attenuated a Murine Model of Atopic Dermatitis through Raising Skin Level of ROS Kim, Hyung-Ran Kim, Jung-Hwan Choi, Eun-Jeong Lee, Yeo Kyong Kie, Jeong-Hae Jang, Myoung Ho Seoh, Ju-Young PLoS One Research Article Atopic dermatitis (AD) is a chronic inflammatory skin disease resulting from excessive stimulation of immune cells. Traditionally, reactive oxygen species (ROS) have been implicated in the progression of inflammatory diseases, but several opposing observations suggest the protective role of ROS in inflammatory disease. Recently, we demonstrated ROS prevented imiquimod-induced psoriatic dermatitis through enhancing regulatory T cell function. Thus, we hypothesized AD might also be attenuated in elevated levels of ROS through tissue hyperoxygenation, such as by hyperbaric oxygen therapy (HBOT) or applying an oxygen-carrying chemical, perfluorodecalin (PFD). Elevated levels of ROS in the skin have been demonstrated directly by staining with dihydroethidum as well as indirectly by immunohistochemistry (IHC) for indoleamine 2,3-dioxygenase (IDO). A murine model of AD was developed by repeated application of a chemical irritant (1% 2,4-dinitrochlorobenzene) and house dust mite (Dermatophagoide farinae) extract on one ear of BALB/c mice. The results showed treatment with HBOT or PFD significantly attenuated AD, comparably with 0.1% prednicarbate without any signs of side effects, such as telangiectasia. The expressions of interleukin-17A and interferon-γ were also decreased in the AD lesions by treatment with HBOT or PFD. Enhanced expression of IDO and reduced level of hypoxia-inducible factor-1α, in association with increased frequency of FoxP3(+) regulatory T cells in the AD lesions, might be involved in the underlying mechanism of oxygen therapy. Taken together, it was suggested that tissue hyperoxygenation, by HBOT or treatment with PFD, might attenuate AD through enhancing skin ROS level. Public Library of Science 2014-10-02 /pmc/articles/PMC4183587/ /pubmed/25275529 http://dx.doi.org/10.1371/journal.pone.0109297 Text en © 2014 Kim et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Kim, Hyung-Ran Kim, Jung-Hwan Choi, Eun-Jeong Lee, Yeo Kyong Kie, Jeong-Hae Jang, Myoung Ho Seoh, Ju-Young Hyperoxygenation Attenuated a Murine Model of Atopic Dermatitis through Raising Skin Level of ROS |
title | Hyperoxygenation Attenuated a Murine Model of Atopic Dermatitis through Raising Skin Level of ROS |
title_full | Hyperoxygenation Attenuated a Murine Model of Atopic Dermatitis through Raising Skin Level of ROS |
title_fullStr | Hyperoxygenation Attenuated a Murine Model of Atopic Dermatitis through Raising Skin Level of ROS |
title_full_unstemmed | Hyperoxygenation Attenuated a Murine Model of Atopic Dermatitis through Raising Skin Level of ROS |
title_short | Hyperoxygenation Attenuated a Murine Model of Atopic Dermatitis through Raising Skin Level of ROS |
title_sort | hyperoxygenation attenuated a murine model of atopic dermatitis through raising skin level of ros |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4183587/ https://www.ncbi.nlm.nih.gov/pubmed/25275529 http://dx.doi.org/10.1371/journal.pone.0109297 |
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