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Protein Apparent Dielectric Constant and Its Temperature Dependence from Remote Chemical Shift Effects
[Image: see text] A NMR protocol is introduced that permits accurate measurement of minute, remote chemical shift perturbations (CSPs), caused by a mutation-induced change in the electric field. Using protein GB3 as a model system, (1)H(N) CSPs in K19A and K19E mutants can be fitted to small changes...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Chemical
Society
2014
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4183759/ https://www.ncbi.nlm.nih.gov/pubmed/25192058 http://dx.doi.org/10.1021/ja505852b |