Rapid identification of Stenotrophomonas maltophilia by peptide nucleic acid fluorescence in situ hybridization

The objective of this study was to develop a novel peptide nucleic acid (PNA) probe for Stenotrophomonas maltophilia identification by fluorescence in situ hybridization (FISH). The probe was evaluated using 33 human and veterinary clinical S. maltophilia isolates and 45 reference strains representi...

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Autores principales: Hansen, N, Rasmussen, A K I, Fiandaca, M J, Kragh, K N, Bjarnsholt, T, Høiby, N, Stender, H, Guardabassi, L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2014
Materias:
New Microbes in Humans – New Resistant Microbes in Humans
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4184662/
https://www.ncbi.nlm.nih.gov/pubmed/25356348
http://dx.doi.org/10.1002/nmi2.38
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author Hansen, N
Rasmussen, A K I
Fiandaca, M J
Kragh, K N
Bjarnsholt, T
Høiby, N
Stender, H
Guardabassi, L
author_facet Hansen, N
Rasmussen, A K I
Fiandaca, M J
Kragh, K N
Bjarnsholt, T
Høiby, N
Stender, H
Guardabassi, L
author_sort Hansen, N
collection PubMed
description The objective of this study was to develop a novel peptide nucleic acid (PNA) probe for Stenotrophomonas maltophilia identification by fluorescence in situ hybridization (FISH). The probe was evaluated using 33 human and veterinary clinical S. maltophilia isolates and 45 reference strains representing common bacterial species in the respiratory tract. The probe displayed 100% sensitivity and 100% specificity on pure cultures and allowed detection in sputum from cystic fibrosis patients. The detection limit was 10(4) CFU/mL in spiked tracheal aspirate and bronchoalveolar lavage from healthy horses. Altogether the study shows that this species-specific PNA FISH probe facilitates rapid detection of S. maltophilia in biological specimens.
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spelling pubmed-41846622014-10-29 Rapid identification of Stenotrophomonas maltophilia by peptide nucleic acid fluorescence in situ hybridization Hansen, N Rasmussen, A K I Fiandaca, M J Kragh, K N Bjarnsholt, T Høiby, N Stender, H Guardabassi, L New Microbes New Infect New Microbes in Humans – New Resistant Microbes in Humans The objective of this study was to develop a novel peptide nucleic acid (PNA) probe for Stenotrophomonas maltophilia identification by fluorescence in situ hybridization (FISH). The probe was evaluated using 33 human and veterinary clinical S. maltophilia isolates and 45 reference strains representing common bacterial species in the respiratory tract. The probe displayed 100% sensitivity and 100% specificity on pure cultures and allowed detection in sputum from cystic fibrosis patients. The detection limit was 10(4) CFU/mL in spiked tracheal aspirate and bronchoalveolar lavage from healthy horses. Altogether the study shows that this species-specific PNA FISH probe facilitates rapid detection of S. maltophilia in biological specimens. BlackWell Publishing Ltd 2014-05 2014-03-25 /pmc/articles/PMC4184662/ /pubmed/25356348 http://dx.doi.org/10.1002/nmi2.38 Text en © 2014 The Authors. New Microbes and New Infections published by John Wiley & Sons Ltd on behalf of the European Society of Clinical Microbiology and Infectious Disease. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle New Microbes in Humans – New Resistant Microbes in Humans
Hansen, N
Rasmussen, A K I
Fiandaca, M J
Kragh, K N
Bjarnsholt, T
Høiby, N
Stender, H
Guardabassi, L
Rapid identification of Stenotrophomonas maltophilia by peptide nucleic acid fluorescence in situ hybridization
title Rapid identification of Stenotrophomonas maltophilia by peptide nucleic acid fluorescence in situ hybridization
title_full Rapid identification of Stenotrophomonas maltophilia by peptide nucleic acid fluorescence in situ hybridization
title_fullStr Rapid identification of Stenotrophomonas maltophilia by peptide nucleic acid fluorescence in situ hybridization
title_full_unstemmed Rapid identification of Stenotrophomonas maltophilia by peptide nucleic acid fluorescence in situ hybridization
title_short Rapid identification of Stenotrophomonas maltophilia by peptide nucleic acid fluorescence in situ hybridization
title_sort rapid identification of stenotrophomonas maltophilia by peptide nucleic acid fluorescence in situ hybridization
topic New Microbes in Humans – New Resistant Microbes in Humans
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4184662/
https://www.ncbi.nlm.nih.gov/pubmed/25356348
http://dx.doi.org/10.1002/nmi2.38
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