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Stable Isotopes in Fish Eye Lenses as Potential Recorders of Trophic and Geographic History

We evaluated eye lenses as potential recorders of stable isotope histories in fish because they consist of metabolically inert optical proteins that are deposited in successive, concentric circles (laminae) much like otolith circuli and tree rings. We conducted four different tests on lenses from re...

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Autores principales: Wallace, Amy A., Hollander, David J., Peebles, Ernst B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4184832/
https://www.ncbi.nlm.nih.gov/pubmed/25279946
http://dx.doi.org/10.1371/journal.pone.0108935
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author Wallace, Amy A.
Hollander, David J.
Peebles, Ernst B.
author_facet Wallace, Amy A.
Hollander, David J.
Peebles, Ernst B.
author_sort Wallace, Amy A.
collection PubMed
description We evaluated eye lenses as potential recorders of stable isotope histories in fish because they consist of metabolically inert optical proteins that are deposited in successive, concentric circles (laminae) much like otolith circuli and tree rings. We conducted four different tests on lenses from red snapper, red grouper, gag, and white grunt. The first test was a low-resolution screening of multiple individuals (4–5 radial groups of laminae per lens, all species except white grunt). Along the radial axis, all individuals exhibited substantial isotopic variability. Red snapper individuals separated into two groups based on δ (15)N and gag separated into two groups based on δ (13)C. Two gag with the greatest variation were chosen for high-resolution temporal analysis using individual laminae from their second eye lenses. The first-order patterns from the high-resolution analysis generally mimicked patterns from the low-resolution screening of grouped laminae, yet the high-resolution plots revealed early-life details that were not apparent in the low-resolution screenings. For the third test, left- versus right-eye variation was compared using high-resolution methods. White grunt left- and right-eye radial isotopic patterns were almost identical for both δ (13)C and δ (15)N, suggesting the variations observed among individual fish were not artifacts. The final test evaluated intra-laminar variation; multiple samples were analyzed from different parts of the same lamina. Seven laminae from three individuals of two species were analyzed in this manner; variations among laminae were found to be much higher than variations within laminae. However, nominal intra-laminar variations were comparable to nominal differences between left and right lenses, suggesting intra-laminar variation established measurement precision. Eye lens isotopes appear to be useful for reconstructing the isotopic histories of individual fish; these histories can be compared with spatially-derived isoscapes to reconstruct individual histories for site fidelity, movement and trophic position.
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spelling pubmed-41848322014-10-07 Stable Isotopes in Fish Eye Lenses as Potential Recorders of Trophic and Geographic History Wallace, Amy A. Hollander, David J. Peebles, Ernst B. PLoS One Research Article We evaluated eye lenses as potential recorders of stable isotope histories in fish because they consist of metabolically inert optical proteins that are deposited in successive, concentric circles (laminae) much like otolith circuli and tree rings. We conducted four different tests on lenses from red snapper, red grouper, gag, and white grunt. The first test was a low-resolution screening of multiple individuals (4–5 radial groups of laminae per lens, all species except white grunt). Along the radial axis, all individuals exhibited substantial isotopic variability. Red snapper individuals separated into two groups based on δ (15)N and gag separated into two groups based on δ (13)C. Two gag with the greatest variation were chosen for high-resolution temporal analysis using individual laminae from their second eye lenses. The first-order patterns from the high-resolution analysis generally mimicked patterns from the low-resolution screening of grouped laminae, yet the high-resolution plots revealed early-life details that were not apparent in the low-resolution screenings. For the third test, left- versus right-eye variation was compared using high-resolution methods. White grunt left- and right-eye radial isotopic patterns were almost identical for both δ (13)C and δ (15)N, suggesting the variations observed among individual fish were not artifacts. The final test evaluated intra-laminar variation; multiple samples were analyzed from different parts of the same lamina. Seven laminae from three individuals of two species were analyzed in this manner; variations among laminae were found to be much higher than variations within laminae. However, nominal intra-laminar variations were comparable to nominal differences between left and right lenses, suggesting intra-laminar variation established measurement precision. Eye lens isotopes appear to be useful for reconstructing the isotopic histories of individual fish; these histories can be compared with spatially-derived isoscapes to reconstruct individual histories for site fidelity, movement and trophic position. Public Library of Science 2014-10-03 /pmc/articles/PMC4184832/ /pubmed/25279946 http://dx.doi.org/10.1371/journal.pone.0108935 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Wallace, Amy A.
Hollander, David J.
Peebles, Ernst B.
Stable Isotopes in Fish Eye Lenses as Potential Recorders of Trophic and Geographic History
title Stable Isotopes in Fish Eye Lenses as Potential Recorders of Trophic and Geographic History
title_full Stable Isotopes in Fish Eye Lenses as Potential Recorders of Trophic and Geographic History
title_fullStr Stable Isotopes in Fish Eye Lenses as Potential Recorders of Trophic and Geographic History
title_full_unstemmed Stable Isotopes in Fish Eye Lenses as Potential Recorders of Trophic and Geographic History
title_short Stable Isotopes in Fish Eye Lenses as Potential Recorders of Trophic and Geographic History
title_sort stable isotopes in fish eye lenses as potential recorders of trophic and geographic history
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4184832/
https://www.ncbi.nlm.nih.gov/pubmed/25279946
http://dx.doi.org/10.1371/journal.pone.0108935
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