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Identification and Molecular Characterization of MYB Transcription Factor Superfamily in C(4) Model Plant Foxtail Millet (Setaria italica L.)

MYB proteins represent one of the largest transcription factor families in plants, playing important roles in diverse developmental and stress-responsive processes. Considering its significance, several genome-wide analyses have been conducted in almost all land plants except foxtail millet. Foxtail...

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Autores principales: Muthamilarasan, Mehanathan, Khandelwal, Rohit, Yadav, Chandra Bhan, Bonthala, Venkata Suresh, Khan, Yusuf, Prasad, Manoj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4184890/
https://www.ncbi.nlm.nih.gov/pubmed/25279462
http://dx.doi.org/10.1371/journal.pone.0109920
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author Muthamilarasan, Mehanathan
Khandelwal, Rohit
Yadav, Chandra Bhan
Bonthala, Venkata Suresh
Khan, Yusuf
Prasad, Manoj
author_facet Muthamilarasan, Mehanathan
Khandelwal, Rohit
Yadav, Chandra Bhan
Bonthala, Venkata Suresh
Khan, Yusuf
Prasad, Manoj
author_sort Muthamilarasan, Mehanathan
collection PubMed
description MYB proteins represent one of the largest transcription factor families in plants, playing important roles in diverse developmental and stress-responsive processes. Considering its significance, several genome-wide analyses have been conducted in almost all land plants except foxtail millet. Foxtail millet (Setaria italica L.) is a model crop for investigating systems biology of millets and bioenergy grasses. Further, the crop is also known for its potential abiotic stress-tolerance. In this context, a comprehensive genome-wide survey was conducted and 209 MYB protein-encoding genes were identified in foxtail millet. All 209 S. italica MYB (SiMYB) genes were physically mapped onto nine chromosomes of foxtail millet. Gene duplication study showed that segmental- and tandem-duplication have occurred in genome resulting in expansion of this gene family. The protein domain investigation classified SiMYB proteins into three classes according to number of MYB repeats present. The phylogenetic analysis categorized SiMYBs into ten groups (I - X). SiMYB-based comparative mapping revealed a maximum orthology between foxtail millet and sorghum, followed by maize, rice and Brachypodium. Heat map analysis showed tissue-specific expression pattern of predominant SiMYB genes. Expression profiling of candidate MYB genes against abiotic stresses and hormone treatments using qRT-PCR revealed specific and/or overlapping expression patterns of SiMYBs. Taken together, the present study provides a foundation for evolutionary and functional characterization of MYB TFs in foxtail millet to dissect their functions in response to environmental stimuli.
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spelling pubmed-41848902014-10-07 Identification and Molecular Characterization of MYB Transcription Factor Superfamily in C(4) Model Plant Foxtail Millet (Setaria italica L.) Muthamilarasan, Mehanathan Khandelwal, Rohit Yadav, Chandra Bhan Bonthala, Venkata Suresh Khan, Yusuf Prasad, Manoj PLoS One Research Article MYB proteins represent one of the largest transcription factor families in plants, playing important roles in diverse developmental and stress-responsive processes. Considering its significance, several genome-wide analyses have been conducted in almost all land plants except foxtail millet. Foxtail millet (Setaria italica L.) is a model crop for investigating systems biology of millets and bioenergy grasses. Further, the crop is also known for its potential abiotic stress-tolerance. In this context, a comprehensive genome-wide survey was conducted and 209 MYB protein-encoding genes were identified in foxtail millet. All 209 S. italica MYB (SiMYB) genes were physically mapped onto nine chromosomes of foxtail millet. Gene duplication study showed that segmental- and tandem-duplication have occurred in genome resulting in expansion of this gene family. The protein domain investigation classified SiMYB proteins into three classes according to number of MYB repeats present. The phylogenetic analysis categorized SiMYBs into ten groups (I - X). SiMYB-based comparative mapping revealed a maximum orthology between foxtail millet and sorghum, followed by maize, rice and Brachypodium. Heat map analysis showed tissue-specific expression pattern of predominant SiMYB genes. Expression profiling of candidate MYB genes against abiotic stresses and hormone treatments using qRT-PCR revealed specific and/or overlapping expression patterns of SiMYBs. Taken together, the present study provides a foundation for evolutionary and functional characterization of MYB TFs in foxtail millet to dissect their functions in response to environmental stimuli. Public Library of Science 2014-10-03 /pmc/articles/PMC4184890/ /pubmed/25279462 http://dx.doi.org/10.1371/journal.pone.0109920 Text en © 2014 Muthamilarasan et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Muthamilarasan, Mehanathan
Khandelwal, Rohit
Yadav, Chandra Bhan
Bonthala, Venkata Suresh
Khan, Yusuf
Prasad, Manoj
Identification and Molecular Characterization of MYB Transcription Factor Superfamily in C(4) Model Plant Foxtail Millet (Setaria italica L.)
title Identification and Molecular Characterization of MYB Transcription Factor Superfamily in C(4) Model Plant Foxtail Millet (Setaria italica L.)
title_full Identification and Molecular Characterization of MYB Transcription Factor Superfamily in C(4) Model Plant Foxtail Millet (Setaria italica L.)
title_fullStr Identification and Molecular Characterization of MYB Transcription Factor Superfamily in C(4) Model Plant Foxtail Millet (Setaria italica L.)
title_full_unstemmed Identification and Molecular Characterization of MYB Transcription Factor Superfamily in C(4) Model Plant Foxtail Millet (Setaria italica L.)
title_short Identification and Molecular Characterization of MYB Transcription Factor Superfamily in C(4) Model Plant Foxtail Millet (Setaria italica L.)
title_sort identification and molecular characterization of myb transcription factor superfamily in c(4) model plant foxtail millet (setaria italica l.)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4184890/
https://www.ncbi.nlm.nih.gov/pubmed/25279462
http://dx.doi.org/10.1371/journal.pone.0109920
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