Microsatellite multiplex assay for the analysis of Atlantic sturgeon populations

We have developed a multiplex assay covering 16 microsatellite loci, amplified in four polymerase chain reaction (PCR) assays, and loaded on the ABI DNA Analyzer in two separate panels. The assay was tested on 603 individuals originating from wild populations and hatchery stocks of Atlantic sturgeon...

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Autores principales: Panagiotopoulou, H., Popovic, D., Zalewska, K., Weglenski, P., Stankovic, A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2014
Materias:
Animal Genetics • Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4185099/
https://www.ncbi.nlm.nih.gov/pubmed/24793446
http://dx.doi.org/10.1007/s13353-014-0216-y
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author Panagiotopoulou, H.
Popovic, D.
Zalewska, K.
Weglenski, P.
Stankovic, A.
author_facet Panagiotopoulou, H.
Popovic, D.
Zalewska, K.
Weglenski, P.
Stankovic, A.
author_sort Panagiotopoulou, H.
collection PubMed
description We have developed a multiplex assay covering 16 microsatellite loci, amplified in four polymerase chain reaction (PCR) assays, and loaded on the ABI DNA Analyzer in two separate panels. The assay was tested on 603 individuals originating from wild populations and hatchery stocks of Atlantic sturgeon. The assay was also tested on 12 individuals of European sturgeon and appeared to be almost equally useful. The multiplex assay designed in this study can be successfully applied in studies requiring high genetic resolution, such as relatedness analysis, selective breeding programs, and stock identification of Atlantic sturgeon. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s13353-014-0216-y) contains supplementary material, which is available to authorized users.
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spelling pubmed-41850992014-10-08 Microsatellite multiplex assay for the analysis of Atlantic sturgeon populations Panagiotopoulou, H. Popovic, D. Zalewska, K. Weglenski, P. Stankovic, A. J Appl Genet Animal Genetics • Short Communication We have developed a multiplex assay covering 16 microsatellite loci, amplified in four polymerase chain reaction (PCR) assays, and loaded on the ABI DNA Analyzer in two separate panels. The assay was tested on 603 individuals originating from wild populations and hatchery stocks of Atlantic sturgeon. The assay was also tested on 12 individuals of European sturgeon and appeared to be almost equally useful. The multiplex assay designed in this study can be successfully applied in studies requiring high genetic resolution, such as relatedness analysis, selective breeding programs, and stock identification of Atlantic sturgeon. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s13353-014-0216-y) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2014-05-04 2014 /pmc/articles/PMC4185099/ /pubmed/24793446 http://dx.doi.org/10.1007/s13353-014-0216-y Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Animal Genetics • Short Communication
Panagiotopoulou, H.
Popovic, D.
Zalewska, K.
Weglenski, P.
Stankovic, A.
Microsatellite multiplex assay for the analysis of Atlantic sturgeon populations
title Microsatellite multiplex assay for the analysis of Atlantic sturgeon populations
title_full Microsatellite multiplex assay for the analysis of Atlantic sturgeon populations
title_fullStr Microsatellite multiplex assay for the analysis of Atlantic sturgeon populations
title_full_unstemmed Microsatellite multiplex assay for the analysis of Atlantic sturgeon populations
title_short Microsatellite multiplex assay for the analysis of Atlantic sturgeon populations
title_sort microsatellite multiplex assay for the analysis of atlantic sturgeon populations
topic Animal Genetics • Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4185099/
https://www.ncbi.nlm.nih.gov/pubmed/24793446
http://dx.doi.org/10.1007/s13353-014-0216-y
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AT weglenskip microsatellitemultiplexassayfortheanalysisofatlanticsturgeonpopulations
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