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Establishment of an animal model of vascular dementia

The aim of this study was to establish a mouse model of vascular dementia (VaD) in order to overcome the shortcomings of rat models of VaD, which include high production costs, difficult surgery, surgical trauma and high mortality. In this study, repeated ischemia-reperfusion of the total bilateral...

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Autor principal: WANG, HAO
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4186350/
https://www.ncbi.nlm.nih.gov/pubmed/25289066
http://dx.doi.org/10.3892/etm.2014.1926
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author WANG, HAO
author_facet WANG, HAO
author_sort WANG, HAO
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description The aim of this study was to establish a mouse model of vascular dementia (VaD) in order to overcome the shortcomings of rat models of VaD, which include high production costs, difficult surgery, surgical trauma and high mortality. In this study, repeated ischemia-reperfusion of the total bilateral carotid artery in mice, combined with a reduction of blood pressure, was used to establish an animal model of VaD. A total of 40 Kunming mice (clean grade) were randomly divided into a sham group and a model group. Behavioral tests were performed for each group following the surgery, and the morphology of the hippocampus was analyzed. The results of the step-down avoidance test, water maze test and microscopy examinations confirmed that following surgery, learning and memory dysfunction was significantly increased in the model group. The results of the morphological observations showed that the number of hippocampal CA1 neurons was significantly decreased in the model group compared with that in the sham surgery group (P<0.01). In the present study, a mouse model of VaD was successfully established, which was simple, effective and reliable, and may be used in the future to investigate VaD.
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spelling pubmed-41863502014-10-06 Establishment of an animal model of vascular dementia WANG, HAO Exp Ther Med Articles The aim of this study was to establish a mouse model of vascular dementia (VaD) in order to overcome the shortcomings of rat models of VaD, which include high production costs, difficult surgery, surgical trauma and high mortality. In this study, repeated ischemia-reperfusion of the total bilateral carotid artery in mice, combined with a reduction of blood pressure, was used to establish an animal model of VaD. A total of 40 Kunming mice (clean grade) were randomly divided into a sham group and a model group. Behavioral tests were performed for each group following the surgery, and the morphology of the hippocampus was analyzed. The results of the step-down avoidance test, water maze test and microscopy examinations confirmed that following surgery, learning and memory dysfunction was significantly increased in the model group. The results of the morphological observations showed that the number of hippocampal CA1 neurons was significantly decreased in the model group compared with that in the sham surgery group (P<0.01). In the present study, a mouse model of VaD was successfully established, which was simple, effective and reliable, and may be used in the future to investigate VaD. D.A. Spandidos 2014-11 2014-08-21 /pmc/articles/PMC4186350/ /pubmed/25289066 http://dx.doi.org/10.3892/etm.2014.1926 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
WANG, HAO
Establishment of an animal model of vascular dementia
title Establishment of an animal model of vascular dementia
title_full Establishment of an animal model of vascular dementia
title_fullStr Establishment of an animal model of vascular dementia
title_full_unstemmed Establishment of an animal model of vascular dementia
title_short Establishment of an animal model of vascular dementia
title_sort establishment of an animal model of vascular dementia
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4186350/
https://www.ncbi.nlm.nih.gov/pubmed/25289066
http://dx.doi.org/10.3892/etm.2014.1926
work_keys_str_mv AT wanghao establishmentofananimalmodelofvasculardementia