Evaluating the performance of a rapid antigen test for the detection of influenza virus in clinical specimens from children in Cameroon

The performance of SD Bioline rapid antigen test (RAT) was evaluated using real-time reverse transcription polymerase chain reaction (rRT-PCR) as gold standard. A total of 718 nasal swabs, including 102 rRT-PCR positive and 616 rRT-PCR negative swabs, were tested. RAT demonstrates a sensitivity of 2...

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Detalles Bibliográficos
Autores principales: Kenmoe, Sebastien, Tchendjou, Patrice, Moyo Tetang, Suzie, Mossus, Tatiana, Njankouo Ripa, Mohammed, Guillet, Marlène, Kfutwah, Anfumbom, Njouom, Richard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2014
Materias:
Short Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4186458/
https://www.ncbi.nlm.nih.gov/pubmed/24266902
http://dx.doi.org/10.1111/irv.12210
Descripción
Sumario:The performance of SD Bioline rapid antigen test (RAT) was evaluated using real-time reverse transcription polymerase chain reaction (rRT-PCR) as gold standard. A total of 718 nasal swabs, including 102 rRT-PCR positive and 616 rRT-PCR negative swabs, were tested. RAT demonstrates a sensitivity of 29·4% with a specificity of 100%. The positivity rate of RAT was highly associated with lower cycle threshold (Ct) values (P < 0·0001). The excellent specificity of the RAT allowed for the rapid identification of influenza cases. However, negative results should be verified by rRT-PCR test because of limitations observed in sensitivity.

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