Evaluating the performance of a rapid antigen test for the detection of influenza virus in clinical specimens from children in Cameroon

The performance of SD Bioline rapid antigen test (RAT) was evaluated using real-time reverse transcription polymerase chain reaction (rRT-PCR) as gold standard. A total of 718 nasal swabs, including 102 rRT-PCR positive and 616 rRT-PCR negative swabs, were tested. RAT demonstrates a sensitivity of 2...

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Autores principales: Kenmoe, Sebastien, Tchendjou, Patrice, Moyo Tetang, Suzie, Mossus, Tatiana, Njankouo Ripa, Mohammed, Guillet, Marlène, Kfutwah, Anfumbom, Njouom, Richard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2014
Materias:
Short Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4186458/
https://www.ncbi.nlm.nih.gov/pubmed/24266902
http://dx.doi.org/10.1111/irv.12210
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author Kenmoe, Sebastien
Tchendjou, Patrice
Moyo Tetang, Suzie
Mossus, Tatiana
Njankouo Ripa, Mohammed
Guillet, Marlène
Kfutwah, Anfumbom
Njouom, Richard
author_facet Kenmoe, Sebastien
Tchendjou, Patrice
Moyo Tetang, Suzie
Mossus, Tatiana
Njankouo Ripa, Mohammed
Guillet, Marlène
Kfutwah, Anfumbom
Njouom, Richard
author_sort Kenmoe, Sebastien
collection PubMed
description The performance of SD Bioline rapid antigen test (RAT) was evaluated using real-time reverse transcription polymerase chain reaction (rRT-PCR) as gold standard. A total of 718 nasal swabs, including 102 rRT-PCR positive and 616 rRT-PCR negative swabs, were tested. RAT demonstrates a sensitivity of 29·4% with a specificity of 100%. The positivity rate of RAT was highly associated with lower cycle threshold (Ct) values (P < 0·0001). The excellent specificity of the RAT allowed for the rapid identification of influenza cases. However, negative results should be verified by rRT-PCR test because of limitations observed in sensitivity.
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spelling pubmed-41864582014-10-29 Evaluating the performance of a rapid antigen test for the detection of influenza virus in clinical specimens from children in Cameroon Kenmoe, Sebastien Tchendjou, Patrice Moyo Tetang, Suzie Mossus, Tatiana Njankouo Ripa, Mohammed Guillet, Marlène Kfutwah, Anfumbom Njouom, Richard Influenza Other Respir Viruses Short Articles The performance of SD Bioline rapid antigen test (RAT) was evaluated using real-time reverse transcription polymerase chain reaction (rRT-PCR) as gold standard. A total of 718 nasal swabs, including 102 rRT-PCR positive and 616 rRT-PCR negative swabs, were tested. RAT demonstrates a sensitivity of 29·4% with a specificity of 100%. The positivity rate of RAT was highly associated with lower cycle threshold (Ct) values (P < 0·0001). The excellent specificity of the RAT allowed for the rapid identification of influenza cases. However, negative results should be verified by rRT-PCR test because of limitations observed in sensitivity. Blackwell Publishing Ltd 2014-03 2013-11-24 /pmc/articles/PMC4186458/ /pubmed/24266902 http://dx.doi.org/10.1111/irv.12210 Text en © 2013 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Articles
Kenmoe, Sebastien
Tchendjou, Patrice
Moyo Tetang, Suzie
Mossus, Tatiana
Njankouo Ripa, Mohammed
Guillet, Marlène
Kfutwah, Anfumbom
Njouom, Richard
Evaluating the performance of a rapid antigen test for the detection of influenza virus in clinical specimens from children in Cameroon
title Evaluating the performance of a rapid antigen test for the detection of influenza virus in clinical specimens from children in Cameroon
title_full Evaluating the performance of a rapid antigen test for the detection of influenza virus in clinical specimens from children in Cameroon
title_fullStr Evaluating the performance of a rapid antigen test for the detection of influenza virus in clinical specimens from children in Cameroon
title_full_unstemmed Evaluating the performance of a rapid antigen test for the detection of influenza virus in clinical specimens from children in Cameroon
title_short Evaluating the performance of a rapid antigen test for the detection of influenza virus in clinical specimens from children in Cameroon
title_sort evaluating the performance of a rapid antigen test for the detection of influenza virus in clinical specimens from children in cameroon
topic Short Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4186458/
https://www.ncbi.nlm.nih.gov/pubmed/24266902
http://dx.doi.org/10.1111/irv.12210
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