Overexpression of the Survivin gene in SGC7901 cell resistance to cisplatin

The aim of the present study was to investigate the mechanism of SGC7901 cell resistance to cisplatin (CDDP). SGC7901/CDDP cells were established by the long-term continuous exposure of SGC7901 cells to CDDP in stepwise concentration increments. The morphologies of the SGC7901/CDDP and SGC7901 cells...

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Autores principales: DONG, HANZHANG, LIU, GAOGAO, JIANG, BIAO, GUO, JIUBING, TAO, GUOQUAN, YIU, WEI, ZHOU, JINGSONG, LI, GUOXIN
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2014
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4186609/
https://www.ncbi.nlm.nih.gov/pubmed/25295077
http://dx.doi.org/10.3892/ol.2014.2463
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author DONG, HANZHANG
LIU, GAOGAO
JIANG, BIAO
GUO, JIUBING
TAO, GUOQUAN
YIU, WEI
ZHOU, JINGSONG
LI, GUOXIN
author_facet DONG, HANZHANG
LIU, GAOGAO
JIANG, BIAO
GUO, JIUBING
TAO, GUOQUAN
YIU, WEI
ZHOU, JINGSONG
LI, GUOXIN
author_sort DONG, HANZHANG
collection PubMed
description The aim of the present study was to investigate the mechanism of SGC7901 cell resistance to cisplatin (CDDP). SGC7901/CDDP cells were established by the long-term continuous exposure of SGC7901 cells to CDDP in stepwise concentration increments. The morphologies of the SGC7901/CDDP and SGC7901 cells were observed by microscopy. The expression levels of Survivin mRNA and protein in the SGC7901/CDDP and SGC7901 cells were examined by reverse transcription polymerase chain reaction and western blotting respectively. The results revealed morphological differences between the SGC7901 and SGC7901/CDDP cells. The expression levels of Survivin mRNA and protein were significantly higher in the SGC7901/CDDP cells than in the SGC7901 cells. Therefore, high expression levels of the Survivin gene may explain SGC7901 cell resistance to CDDP.
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spelling pubmed-41866092014-10-07 Overexpression of the Survivin gene in SGC7901 cell resistance to cisplatin DONG, HANZHANG LIU, GAOGAO JIANG, BIAO GUO, JIUBING TAO, GUOQUAN YIU, WEI ZHOU, JINGSONG LI, GUOXIN Oncol Lett Articles The aim of the present study was to investigate the mechanism of SGC7901 cell resistance to cisplatin (CDDP). SGC7901/CDDP cells were established by the long-term continuous exposure of SGC7901 cells to CDDP in stepwise concentration increments. The morphologies of the SGC7901/CDDP and SGC7901 cells were observed by microscopy. The expression levels of Survivin mRNA and protein in the SGC7901/CDDP and SGC7901 cells were examined by reverse transcription polymerase chain reaction and western blotting respectively. The results revealed morphological differences between the SGC7901 and SGC7901/CDDP cells. The expression levels of Survivin mRNA and protein were significantly higher in the SGC7901/CDDP cells than in the SGC7901 cells. Therefore, high expression levels of the Survivin gene may explain SGC7901 cell resistance to CDDP. D.A. Spandidos 2014-11 2014-08-20 /pmc/articles/PMC4186609/ /pubmed/25295077 http://dx.doi.org/10.3892/ol.2014.2463 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
DONG, HANZHANG
LIU, GAOGAO
JIANG, BIAO
GUO, JIUBING
TAO, GUOQUAN
YIU, WEI
ZHOU, JINGSONG
LI, GUOXIN
Overexpression of the Survivin gene in SGC7901 cell resistance to cisplatin
title Overexpression of the Survivin gene in SGC7901 cell resistance to cisplatin
title_full Overexpression of the Survivin gene in SGC7901 cell resistance to cisplatin
title_fullStr Overexpression of the Survivin gene in SGC7901 cell resistance to cisplatin
title_full_unstemmed Overexpression of the Survivin gene in SGC7901 cell resistance to cisplatin
title_short Overexpression of the Survivin gene in SGC7901 cell resistance to cisplatin
title_sort overexpression of the survivin gene in sgc7901 cell resistance to cisplatin
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4186609/
https://www.ncbi.nlm.nih.gov/pubmed/25295077
http://dx.doi.org/10.3892/ol.2014.2463
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