Understanding the Role of Histidine in the GHSxG Acyltransferase Active Site Motif: Evidence for Histidine Stabilization of the Malonyl-Enzyme Intermediate

Acyltransferases determine which extender units are incorporated into polyketide and fatty acid products. The ping-pong acyltransferase mechanism utilizes a serine in a conserved GHSxG motif. However, the role of the conserved histidine in this motif is poorly understood. We observed that a histidin...

Descripción completa

Detalles Bibliográficos
Autores principales: Poust, Sean, Yoon, Isu, Adams, Paul D., Katz, Leonard, Petzold, Christopher J., Keasling, Jay D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4186864/
https://www.ncbi.nlm.nih.gov/pubmed/25286165
http://dx.doi.org/10.1371/journal.pone.0109421

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4186864/
https://www.ncbi.nlm.nih.gov/pubmed/25286165
http://dx.doi.org/10.1371/journal.pone.0109421

Ejemplares similares