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Cineradiographic Analysis of Mouse Postural Response to Alteration of Gravity and Jerk (Gravity Deceleration Rate)

The ability to maintain the body relative to the external environment is important for adaptation to altered gravity. However, the physiological limits for adaptation or the disruption of body orientation are not known. In this study, we analyzed postural changes in mice upon exposure to various low...

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Autores principales: Hasegawa, Katsuya, de Campos, Priscila S., Zeredo, Jorge L., Kumei, Yasuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4187164/
https://www.ncbi.nlm.nih.gov/pubmed/25370191
http://dx.doi.org/10.3390/life4020174
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author Hasegawa, Katsuya
de Campos, Priscila S.
Zeredo, Jorge L.
Kumei, Yasuhiro
author_facet Hasegawa, Katsuya
de Campos, Priscila S.
Zeredo, Jorge L.
Kumei, Yasuhiro
author_sort Hasegawa, Katsuya
collection PubMed
description The ability to maintain the body relative to the external environment is important for adaptation to altered gravity. However, the physiological limits for adaptation or the disruption of body orientation are not known. In this study, we analyzed postural changes in mice upon exposure to various low gravities. Male C57BL6/J mice (n = 6) were exposed to various gravity-deceleration conditions by customized parabolic flight-maneuvers targeting the partial-gravity levels of 0.60, 0.30, 0.15 and μ g (<0.001 g). Video recordings of postural responses were analyzed frame-by-frame by high-definition cineradiography and with exact instantaneous values of gravity and jerk. As a result, the coordinated extension of the neck, spine and hindlimbs was observed during the initial phase of gravity deceleration. Joint angles widened to 120%–200% of the reference g level, and the magnitude of the thoracic-curvature stretching was correlated with gravity and jerk, i.e., the gravity deceleration rate. A certain range of jerk facilitated mouse skeletal stretching efficiently, and a jerk of −0.3~−0.4 j (g/s) induced the maximum extension of the thoracic-curvature. The postural response of animals to low gravity may undergo differential regulation by gravity and jerk.
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spelling pubmed-41871642014-10-27 Cineradiographic Analysis of Mouse Postural Response to Alteration of Gravity and Jerk (Gravity Deceleration Rate) Hasegawa, Katsuya de Campos, Priscila S. Zeredo, Jorge L. Kumei, Yasuhiro Life (Basel) Article The ability to maintain the body relative to the external environment is important for adaptation to altered gravity. However, the physiological limits for adaptation or the disruption of body orientation are not known. In this study, we analyzed postural changes in mice upon exposure to various low gravities. Male C57BL6/J mice (n = 6) were exposed to various gravity-deceleration conditions by customized parabolic flight-maneuvers targeting the partial-gravity levels of 0.60, 0.30, 0.15 and μ g (<0.001 g). Video recordings of postural responses were analyzed frame-by-frame by high-definition cineradiography and with exact instantaneous values of gravity and jerk. As a result, the coordinated extension of the neck, spine and hindlimbs was observed during the initial phase of gravity deceleration. Joint angles widened to 120%–200% of the reference g level, and the magnitude of the thoracic-curvature stretching was correlated with gravity and jerk, i.e., the gravity deceleration rate. A certain range of jerk facilitated mouse skeletal stretching efficiently, and a jerk of −0.3~−0.4 j (g/s) induced the maximum extension of the thoracic-curvature. The postural response of animals to low gravity may undergo differential regulation by gravity and jerk. MDPI 2014-04-24 /pmc/articles/PMC4187164/ /pubmed/25370191 http://dx.doi.org/10.3390/life4020174 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Hasegawa, Katsuya
de Campos, Priscila S.
Zeredo, Jorge L.
Kumei, Yasuhiro
Cineradiographic Analysis of Mouse Postural Response to Alteration of Gravity and Jerk (Gravity Deceleration Rate)
title Cineradiographic Analysis of Mouse Postural Response to Alteration of Gravity and Jerk (Gravity Deceleration Rate)
title_full Cineradiographic Analysis of Mouse Postural Response to Alteration of Gravity and Jerk (Gravity Deceleration Rate)
title_fullStr Cineradiographic Analysis of Mouse Postural Response to Alteration of Gravity and Jerk (Gravity Deceleration Rate)
title_full_unstemmed Cineradiographic Analysis of Mouse Postural Response to Alteration of Gravity and Jerk (Gravity Deceleration Rate)
title_short Cineradiographic Analysis of Mouse Postural Response to Alteration of Gravity and Jerk (Gravity Deceleration Rate)
title_sort cineradiographic analysis of mouse postural response to alteration of gravity and jerk (gravity deceleration rate)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4187164/
https://www.ncbi.nlm.nih.gov/pubmed/25370191
http://dx.doi.org/10.3390/life4020174
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