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Structure of a short-chain dehydrogenase/reductase (SDR) within a genomic island from a clinical strain of Acinetobacter baumannii

Over 15% of the genome of an Australian clinical isolate of Acinetobacter baumannii occurs within genomic islands. An uncharacterized protein encoded within one island feature common to this and other International Clone II strains has been studied by X-ray crystallography. The 2.4 Å resolution stru...

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Autores principales: Shah, Bhumika S., Tetu, Sasha G., Harrop, Stephen J., Paulsen, Ian T., Mabbutt, Bridget C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4188072/
https://www.ncbi.nlm.nih.gov/pubmed/25286932
http://dx.doi.org/10.1107/S2053230X14019785
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author Shah, Bhumika S.
Tetu, Sasha G.
Harrop, Stephen J.
Paulsen, Ian T.
Mabbutt, Bridget C.
author_facet Shah, Bhumika S.
Tetu, Sasha G.
Harrop, Stephen J.
Paulsen, Ian T.
Mabbutt, Bridget C.
author_sort Shah, Bhumika S.
collection PubMed
description Over 15% of the genome of an Australian clinical isolate of Acinetobacter baumannii occurs within genomic islands. An uncharacterized protein encoded within one island feature common to this and other International Clone II strains has been studied by X-ray crystallography. The 2.4 Å resolution structure of SDR-WM99c reveals it to be a new member of the classical short-chain dehydrogenase/reductase (SDR) superfamily. The enzyme contains a nucleotide-binding domain and, like many other SDRs, is tetrameric in form. The active site contains a catalytic tetrad (Asn117, Ser146, Tyr159 and Lys163) and water molecules occupying the presumed NADP cofactor-binding pocket. An adjacent cleft is capped by a relatively mobile helical subdomain, which is well positioned to control substrate access.
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spelling pubmed-41880722014-10-24 Structure of a short-chain dehydrogenase/reductase (SDR) within a genomic island from a clinical strain of Acinetobacter baumannii Shah, Bhumika S. Tetu, Sasha G. Harrop, Stephen J. Paulsen, Ian T. Mabbutt, Bridget C. Acta Crystallogr F Struct Biol Commun Structural Communications Over 15% of the genome of an Australian clinical isolate of Acinetobacter baumannii occurs within genomic islands. An uncharacterized protein encoded within one island feature common to this and other International Clone II strains has been studied by X-ray crystallography. The 2.4 Å resolution structure of SDR-WM99c reveals it to be a new member of the classical short-chain dehydrogenase/reductase (SDR) superfamily. The enzyme contains a nucleotide-binding domain and, like many other SDRs, is tetrameric in form. The active site contains a catalytic tetrad (Asn117, Ser146, Tyr159 and Lys163) and water molecules occupying the presumed NADP cofactor-binding pocket. An adjacent cleft is capped by a relatively mobile helical subdomain, which is well positioned to control substrate access. International Union of Crystallography 2014-09-25 /pmc/articles/PMC4188072/ /pubmed/25286932 http://dx.doi.org/10.1107/S2053230X14019785 Text en © Shah et al. 2014 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Structural Communications
Shah, Bhumika S.
Tetu, Sasha G.
Harrop, Stephen J.
Paulsen, Ian T.
Mabbutt, Bridget C.
Structure of a short-chain dehydrogenase/reductase (SDR) within a genomic island from a clinical strain of Acinetobacter baumannii
title Structure of a short-chain dehydrogenase/reductase (SDR) within a genomic island from a clinical strain of Acinetobacter baumannii
title_full Structure of a short-chain dehydrogenase/reductase (SDR) within a genomic island from a clinical strain of Acinetobacter baumannii
title_fullStr Structure of a short-chain dehydrogenase/reductase (SDR) within a genomic island from a clinical strain of Acinetobacter baumannii
title_full_unstemmed Structure of a short-chain dehydrogenase/reductase (SDR) within a genomic island from a clinical strain of Acinetobacter baumannii
title_short Structure of a short-chain dehydrogenase/reductase (SDR) within a genomic island from a clinical strain of Acinetobacter baumannii
title_sort structure of a short-chain dehydrogenase/reductase (sdr) within a genomic island from a clinical strain of acinetobacter baumannii
topic Structural Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4188072/
https://www.ncbi.nlm.nih.gov/pubmed/25286932
http://dx.doi.org/10.1107/S2053230X14019785
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