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MicroRNA Detection in Cervical Exfoliated Cells as a Triage for Human Papillomavirus–Positive Women
BACKGROUND: Papanicolaou (Pap) triage, with high specificity, has been recommended for primary Human papillomavirus (HPV) testing but is flawed by poor sensitivity and cytologist dependence. We evaluated the potential role of microRNA (miRNA) detection in cervical exfoliated cells in HPV-positive wo...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4188123/ https://www.ncbi.nlm.nih.gov/pubmed/25190727 http://dx.doi.org/10.1093/jnci/dju241 |
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author | Tian, Qifang Li, Yang Wang, Fenfen Li, Ying Xu, Junfen Shen, Yuanming Ye, Feng Wang, Xinyu Cheng, Xiaodong Chen, Yaxia Wan, Xiaoyun Lu, Weiguo Xie, Xing |
author_facet | Tian, Qifang Li, Yang Wang, Fenfen Li, Ying Xu, Junfen Shen, Yuanming Ye, Feng Wang, Xinyu Cheng, Xiaodong Chen, Yaxia Wan, Xiaoyun Lu, Weiguo Xie, Xing |
author_sort | Tian, Qifang |
collection | PubMed |
description | BACKGROUND: Papanicolaou (Pap) triage, with high specificity, has been recommended for primary Human papillomavirus (HPV) testing but is flawed by poor sensitivity and cytologist dependence. We evaluated the potential role of microRNA (miRNA) detection in cervical exfoliated cells in HPV-positive women from a clinic-based population. METHODS: Primary HPV testing as well as Pap test were performed on all eligible women. Six miRNAs (miR-424/miR-375/miR-34a/miR-218/miR-92a/miR-93) were detected by RT-qPCR in cervical exfoliated cells. All HPV-positive women underwent colposcopy and further biopsy if indicated. Mann–Whitney U test, the receiver operating characteristic curve, logistic regression, and Pearson’s Chi-square were used to assess data. All tests of statistical significance were two-sided. RESULTS: A total of 1021 eligible HPV-positive women were enrolled. The expression of miR-424/miR-375/miR-34a/miR-218 in high-grade cervical intraepithelial neoplasia (CIN) and abnormal cytology was statistically significantly lower than that in low-grade CIN and normal cytology, respectively (all P < .05). Compared with the Pap test, both miR-424 and miR-375 detection achieved higher sensitivity (76.0% and 74.9% vs 63.8%, P < .05), higher negative predictive value (NPV) (85.7% and 85.4% vs 79.3%, P < .05), and comparable specificity while identifying CIN2 or worse (CIN2+). Similar results were achieved while identifying CIN3+. Multi-marker panels based on miR-424, miR-375, and miR-218 further improved the performance over any single miRNA test or Pap test. CONCLUSION: Single miR-424 or miR-375 detection and miR-424/miR-375/miR-218–based multimarker panels in cervical exfoliated cells show superior performance over Pap triage for high-grade CIN identification in a clinic-based population. Detection of miRNA may provide a new triage option for HPV-positive women. |
format | Online Article Text |
id | pubmed-4188123 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-41881232014-10-16 MicroRNA Detection in Cervical Exfoliated Cells as a Triage for Human Papillomavirus–Positive Women Tian, Qifang Li, Yang Wang, Fenfen Li, Ying Xu, Junfen Shen, Yuanming Ye, Feng Wang, Xinyu Cheng, Xiaodong Chen, Yaxia Wan, Xiaoyun Lu, Weiguo Xie, Xing J Natl Cancer Inst Article BACKGROUND: Papanicolaou (Pap) triage, with high specificity, has been recommended for primary Human papillomavirus (HPV) testing but is flawed by poor sensitivity and cytologist dependence. We evaluated the potential role of microRNA (miRNA) detection in cervical exfoliated cells in HPV-positive women from a clinic-based population. METHODS: Primary HPV testing as well as Pap test were performed on all eligible women. Six miRNAs (miR-424/miR-375/miR-34a/miR-218/miR-92a/miR-93) were detected by RT-qPCR in cervical exfoliated cells. All HPV-positive women underwent colposcopy and further biopsy if indicated. Mann–Whitney U test, the receiver operating characteristic curve, logistic regression, and Pearson’s Chi-square were used to assess data. All tests of statistical significance were two-sided. RESULTS: A total of 1021 eligible HPV-positive women were enrolled. The expression of miR-424/miR-375/miR-34a/miR-218 in high-grade cervical intraepithelial neoplasia (CIN) and abnormal cytology was statistically significantly lower than that in low-grade CIN and normal cytology, respectively (all P < .05). Compared with the Pap test, both miR-424 and miR-375 detection achieved higher sensitivity (76.0% and 74.9% vs 63.8%, P < .05), higher negative predictive value (NPV) (85.7% and 85.4% vs 79.3%, P < .05), and comparable specificity while identifying CIN2 or worse (CIN2+). Similar results were achieved while identifying CIN3+. Multi-marker panels based on miR-424, miR-375, and miR-218 further improved the performance over any single miRNA test or Pap test. CONCLUSION: Single miR-424 or miR-375 detection and miR-424/miR-375/miR-218–based multimarker panels in cervical exfoliated cells show superior performance over Pap triage for high-grade CIN identification in a clinic-based population. Detection of miRNA may provide a new triage option for HPV-positive women. Oxford University Press 2014-09-04 /pmc/articles/PMC4188123/ /pubmed/25190727 http://dx.doi.org/10.1093/jnci/dju241 Text en © The Author 2014. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Article Tian, Qifang Li, Yang Wang, Fenfen Li, Ying Xu, Junfen Shen, Yuanming Ye, Feng Wang, Xinyu Cheng, Xiaodong Chen, Yaxia Wan, Xiaoyun Lu, Weiguo Xie, Xing MicroRNA Detection in Cervical Exfoliated Cells as a Triage for Human Papillomavirus–Positive Women |
title | MicroRNA Detection in Cervical Exfoliated Cells as a Triage for Human Papillomavirus–Positive Women |
title_full | MicroRNA Detection in Cervical Exfoliated Cells as a Triage for Human Papillomavirus–Positive Women |
title_fullStr | MicroRNA Detection in Cervical Exfoliated Cells as a Triage for Human Papillomavirus–Positive Women |
title_full_unstemmed | MicroRNA Detection in Cervical Exfoliated Cells as a Triage for Human Papillomavirus–Positive Women |
title_short | MicroRNA Detection in Cervical Exfoliated Cells as a Triage for Human Papillomavirus–Positive Women |
title_sort | microrna detection in cervical exfoliated cells as a triage for human papillomavirus–positive women |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4188123/ https://www.ncbi.nlm.nih.gov/pubmed/25190727 http://dx.doi.org/10.1093/jnci/dju241 |
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