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A Transcriptome for the Study of Early Processes of Retinal Regeneration in the Adult Newt, Cynops pyrrhogaster

Retinal regeneration in the adult newt is a useful system to uncover essential mechanisms underlying the regeneration of body parts of this animal as well as to find clues to treat retinal disorders such as proliferative vitreoretinopathy. Here, to facilitate the study of early processes of retinal...

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Autores principales: Nakamura, Kenta, Islam, Md. Rafiqul, Takayanagi, Miyako, Yasumuro, Hirofumi, Inami, Wataru, Kunahong, Ailidana, Casco-Robles, Roman M., Toyama, Fubito, Chiba, Chikafumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4188609/
https://www.ncbi.nlm.nih.gov/pubmed/25290450
http://dx.doi.org/10.1371/journal.pone.0109831
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author Nakamura, Kenta
Islam, Md. Rafiqul
Takayanagi, Miyako
Yasumuro, Hirofumi
Inami, Wataru
Kunahong, Ailidana
Casco-Robles, Roman M.
Toyama, Fubito
Chiba, Chikafumi
author_facet Nakamura, Kenta
Islam, Md. Rafiqul
Takayanagi, Miyako
Yasumuro, Hirofumi
Inami, Wataru
Kunahong, Ailidana
Casco-Robles, Roman M.
Toyama, Fubito
Chiba, Chikafumi
author_sort Nakamura, Kenta
collection PubMed
description Retinal regeneration in the adult newt is a useful system to uncover essential mechanisms underlying the regeneration of body parts of this animal as well as to find clues to treat retinal disorders such as proliferative vitreoretinopathy. Here, to facilitate the study of early processes of retinal regeneration, we provide a de novo assembly transcriptome and inferred proteome of the Japanese fire bellied newt (Cynops pyrrhogaster), which was obtained from eyeball samples of day 0–14 after surgical removal of the lens and neural retina. This transcriptome (237,120 in silico transcripts) contains most information of cDNAs/ESTs which has been reported in newts (C. pyrrhogaster, Pleurodeles waltl and Notophthalmus viridescence) thus far. On the other hand, de novo assembly transcriptomes reported lately for N. viridescence only covered 16–31% of this transcriptome, suggesting that most constituents of this transcriptome are specific to the regenerating eye tissues of C. pyrrhogaster. A total of 87,102 in silico transcripts of this transcriptome were functionally annotated. Coding sequence prediction in combination with functional annotation revealed that 76,968 in silico transcripts encode protein/peptides recorded in public databases so far, whereas 17,316 might be unique. qPCR and Sanger sequencing demonstrated that this transcriptome contains much information pertaining to genes that are regulated in association with cell reprogramming, cell-cycle re-entry/proliferation, and tissue patterning in an early phase of retinal regeneration. This data also provides important insight for further investigations addressing cellular mechanisms and molecular networks underlying retinal regeneration as well as differences between retinal regeneration and disorders. This transcriptome can be applied to ensuing comprehensive gene screening steps, providing candidate genes, regardless of whether annotated or unique, to uncover essential mechanisms underlying early processes of retinal regeneration.
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spelling pubmed-41886092014-10-10 A Transcriptome for the Study of Early Processes of Retinal Regeneration in the Adult Newt, Cynops pyrrhogaster Nakamura, Kenta Islam, Md. Rafiqul Takayanagi, Miyako Yasumuro, Hirofumi Inami, Wataru Kunahong, Ailidana Casco-Robles, Roman M. Toyama, Fubito Chiba, Chikafumi PLoS One Research Article Retinal regeneration in the adult newt is a useful system to uncover essential mechanisms underlying the regeneration of body parts of this animal as well as to find clues to treat retinal disorders such as proliferative vitreoretinopathy. Here, to facilitate the study of early processes of retinal regeneration, we provide a de novo assembly transcriptome and inferred proteome of the Japanese fire bellied newt (Cynops pyrrhogaster), which was obtained from eyeball samples of day 0–14 after surgical removal of the lens and neural retina. This transcriptome (237,120 in silico transcripts) contains most information of cDNAs/ESTs which has been reported in newts (C. pyrrhogaster, Pleurodeles waltl and Notophthalmus viridescence) thus far. On the other hand, de novo assembly transcriptomes reported lately for N. viridescence only covered 16–31% of this transcriptome, suggesting that most constituents of this transcriptome are specific to the regenerating eye tissues of C. pyrrhogaster. A total of 87,102 in silico transcripts of this transcriptome were functionally annotated. Coding sequence prediction in combination with functional annotation revealed that 76,968 in silico transcripts encode protein/peptides recorded in public databases so far, whereas 17,316 might be unique. qPCR and Sanger sequencing demonstrated that this transcriptome contains much information pertaining to genes that are regulated in association with cell reprogramming, cell-cycle re-entry/proliferation, and tissue patterning in an early phase of retinal regeneration. This data also provides important insight for further investigations addressing cellular mechanisms and molecular networks underlying retinal regeneration as well as differences between retinal regeneration and disorders. This transcriptome can be applied to ensuing comprehensive gene screening steps, providing candidate genes, regardless of whether annotated or unique, to uncover essential mechanisms underlying early processes of retinal regeneration. Public Library of Science 2014-10-07 /pmc/articles/PMC4188609/ /pubmed/25290450 http://dx.doi.org/10.1371/journal.pone.0109831 Text en © 2014 Nakamura et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Nakamura, Kenta
Islam, Md. Rafiqul
Takayanagi, Miyako
Yasumuro, Hirofumi
Inami, Wataru
Kunahong, Ailidana
Casco-Robles, Roman M.
Toyama, Fubito
Chiba, Chikafumi
A Transcriptome for the Study of Early Processes of Retinal Regeneration in the Adult Newt, Cynops pyrrhogaster
title A Transcriptome for the Study of Early Processes of Retinal Regeneration in the Adult Newt, Cynops pyrrhogaster
title_full A Transcriptome for the Study of Early Processes of Retinal Regeneration in the Adult Newt, Cynops pyrrhogaster
title_fullStr A Transcriptome for the Study of Early Processes of Retinal Regeneration in the Adult Newt, Cynops pyrrhogaster
title_full_unstemmed A Transcriptome for the Study of Early Processes of Retinal Regeneration in the Adult Newt, Cynops pyrrhogaster
title_short A Transcriptome for the Study of Early Processes of Retinal Regeneration in the Adult Newt, Cynops pyrrhogaster
title_sort transcriptome for the study of early processes of retinal regeneration in the adult newt, cynops pyrrhogaster
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4188609/
https://www.ncbi.nlm.nih.gov/pubmed/25290450
http://dx.doi.org/10.1371/journal.pone.0109831
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