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Total extract of Korean red ginseng facilitates human bone marrow hematopoietic colony formation in vitro
BACKGROUND: The number of CD34+ cells in a peripheral blood stem cell collection is the key factor in predicting successful treatment of hematologic malignancies. Korean Red Ginseng (KRG) (Panax ginseng C.A. Meyer) is the most popular medicinal herb in Korea. The objective of this study was to deter...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society of Hematology; Korean Society of Blood and Marrow Transplantation; Korean Society of Pediatric Hematology-Oncology; Korean Society on Thrombosis and Hemostasis
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4188783/ https://www.ncbi.nlm.nih.gov/pubmed/25325037 http://dx.doi.org/10.5045/br.2014.49.3.177 |
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author | Kim, Sang-Gyung Lee, A-Jin Bae, Sung Hwa Kim, Seong-Mo Lee, Ji-Hye Kim, Min Ji Jang, Hae-Bong |
author_facet | Kim, Sang-Gyung Lee, A-Jin Bae, Sung Hwa Kim, Seong-Mo Lee, Ji-Hye Kim, Min Ji Jang, Hae-Bong |
author_sort | Kim, Sang-Gyung |
collection | PubMed |
description | BACKGROUND: The number of CD34+ cells in a peripheral blood stem cell collection is the key factor in predicting successful treatment of hematologic malignancies. Korean Red Ginseng (KRG) (Panax ginseng C.A. Meyer) is the most popular medicinal herb in Korea. The objective of this study was to determine the effect of KRG on hematopoietic colony formation. METHODS: Bone marrow (BM) samples were obtained from 8 human donors after acquiring informed consent. BM mononuclear cells (MNCs) were isolated, and CD34+ cells were sorted using magnetic beads. The sorted CD34+ cells were incubated with or without total extract of KRG (50 µg/mL, 100 µg/mL) or Ginsenoside Rg1 (100 µg/mL), and the hematopoietic colony assay was performed using methylcellulose semisolid medium. The CD34+ cell counts were measured by a single platform assay using flow cytometry. RESULTS: The numbers of human BM-MNCs and CD34+ cells obtained after purification were variable among donors (5.6×10(7) and 1.3-48×10(7) and 8.9×10(4) and 1.8-80×10(4), respectively). The cells expanded 1,944 times after incubation for 12 d. Total extract of KRG added to the hematopoietic stem cell (HSC)-specific medium increased CD34+ cell counts 3.6 times compared to 2.6 times when using HSC medium alone. Total numbers of hematopoietic colonies in KRG medium were more than those observed in conventional medium, especially that of erythroid colonies such as burst forming unit-erythroid. CONCLUSION: Total extract of KRG facilitated CD34+ cell expansion and hematopoietic colony formation, especially of the erythroid lineage. |
format | Online Article Text |
id | pubmed-4188783 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Korean Society of Hematology; Korean Society of Blood and Marrow Transplantation; Korean Society of Pediatric Hematology-Oncology; Korean Society on Thrombosis and Hemostasis |
record_format | MEDLINE/PubMed |
spelling | pubmed-41887832014-10-16 Total extract of Korean red ginseng facilitates human bone marrow hematopoietic colony formation in vitro Kim, Sang-Gyung Lee, A-Jin Bae, Sung Hwa Kim, Seong-Mo Lee, Ji-Hye Kim, Min Ji Jang, Hae-Bong Blood Res Original Article BACKGROUND: The number of CD34+ cells in a peripheral blood stem cell collection is the key factor in predicting successful treatment of hematologic malignancies. Korean Red Ginseng (KRG) (Panax ginseng C.A. Meyer) is the most popular medicinal herb in Korea. The objective of this study was to determine the effect of KRG on hematopoietic colony formation. METHODS: Bone marrow (BM) samples were obtained from 8 human donors after acquiring informed consent. BM mononuclear cells (MNCs) were isolated, and CD34+ cells were sorted using magnetic beads. The sorted CD34+ cells were incubated with or without total extract of KRG (50 µg/mL, 100 µg/mL) or Ginsenoside Rg1 (100 µg/mL), and the hematopoietic colony assay was performed using methylcellulose semisolid medium. The CD34+ cell counts were measured by a single platform assay using flow cytometry. RESULTS: The numbers of human BM-MNCs and CD34+ cells obtained after purification were variable among donors (5.6×10(7) and 1.3-48×10(7) and 8.9×10(4) and 1.8-80×10(4), respectively). The cells expanded 1,944 times after incubation for 12 d. Total extract of KRG added to the hematopoietic stem cell (HSC)-specific medium increased CD34+ cell counts 3.6 times compared to 2.6 times when using HSC medium alone. Total numbers of hematopoietic colonies in KRG medium were more than those observed in conventional medium, especially that of erythroid colonies such as burst forming unit-erythroid. CONCLUSION: Total extract of KRG facilitated CD34+ cell expansion and hematopoietic colony formation, especially of the erythroid lineage. Korean Society of Hematology; Korean Society of Blood and Marrow Transplantation; Korean Society of Pediatric Hematology-Oncology; Korean Society on Thrombosis and Hemostasis 2014-09 2014-09-25 /pmc/articles/PMC4188783/ /pubmed/25325037 http://dx.doi.org/10.5045/br.2014.49.3.177 Text en © 2014 Korean Society of Hematology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kim, Sang-Gyung Lee, A-Jin Bae, Sung Hwa Kim, Seong-Mo Lee, Ji-Hye Kim, Min Ji Jang, Hae-Bong Total extract of Korean red ginseng facilitates human bone marrow hematopoietic colony formation in vitro |
title | Total extract of Korean red ginseng facilitates human bone marrow hematopoietic colony formation in vitro |
title_full | Total extract of Korean red ginseng facilitates human bone marrow hematopoietic colony formation in vitro |
title_fullStr | Total extract of Korean red ginseng facilitates human bone marrow hematopoietic colony formation in vitro |
title_full_unstemmed | Total extract of Korean red ginseng facilitates human bone marrow hematopoietic colony formation in vitro |
title_short | Total extract of Korean red ginseng facilitates human bone marrow hematopoietic colony formation in vitro |
title_sort | total extract of korean red ginseng facilitates human bone marrow hematopoietic colony formation in vitro |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4188783/ https://www.ncbi.nlm.nih.gov/pubmed/25325037 http://dx.doi.org/10.5045/br.2014.49.3.177 |
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